In this paper, we study the singularly perturbed Laguerre unitary ensemblewith V t (x) = x + t/x, x ∈ (0, +∞) and t > 0. Due to the effect of t/x for varying t, the eigenvalue correlation kernel has a new limit instead of the usual Bessel kernel at the hard edge 0. This limiting kernel involves ψ-functions associated with a special solution to a new thirdorder nonlinear differential equation, which is then shown equivalent to a particular Painlevé III equation. The transition of this limiting kernel to the Bessel and Airy kernels is also studied when the parameter t changes in a finite interval (0, d]. Our approach is based on Deift-Zhou nonlinear steepest descent method for Riemann-Hilbert problems.