Global changes in metabolic pathways in endocarp of ‘Dayagan’ hybrid citrus fruit during segment drying revealed by widely targeted metabolomics and transcriptomics analysis

Drying enhances the antioxidant activity of Allium mongolicum Regel through the phenylpropane and AA-MA pathway as shown by metabolomics

“…A broadly targeted metabolomics study to reveal the transformation mechanism of active compounds in Cistanche during the drying process revealed that the biosynthesis of key metabolites, such as phenylalanine and flavonoids, was significantly enhanced during the drying process, and the production of amino acids and unsaturated fatty acids increased in Cistanche, suggesting that there is a reciprocal transformation relationship between the primary and secondary metabolites of Cistanche ( Ai, Zhang, Li, Sun, & Liu, 2021 ). Similarly, Hou et al (2023) used metabolomics and transcriptomics analyses to reveal the metabolic pathways of endocarp during segmental drying of ‘Dayagan’ hybrid citrus, and the changes in the endocarp were correlated with the loss of water during drying. Metabolomics results confirmed that differential metabolites were enriched in metabolic pathways related to amino acids and their derivatives, lipids and flavonoids synthesis, and up-regulated genes confirmed that the phenylpropane pathway was activated during drying to participate in the accumulation of flavonoids and other active constituents.…”