Global Chromosome Topology and the Two-Component Systems in Concerted Manner Regulate Transcription in
            <i>Streptomyces</i>

Gongerowska-Jac, Martyna; Szafran, Marcin Jan; Mikołajczyk, Jakub; Szymczak, Justyna; Bartyńska, Magdalena; Gierlikowska, Anna; Biały, Sylwia; Elliot, Marie A.; Jakimowicz, Dagmara

doi:10.1128/msystems.01142-21

Cited by 3 publications

(4 citation statements)

References 82 publications

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“…Our current procedure of using relaxed DNA may overlook gene expression that requires a specific state of topology. Indeed, elevated chromosome supercoiling caused by topoisomerase I depletion changes global gene expression in S. coelicolor A3(2) 40 . In certain cases, it may be important or sometimes necessary to use undigested genomic DNA and add topoisomerase or gyrase to the in vitro transcription reaction.…”

Section: Discussionmentioning

confidence: 99%

RIViT-seq enables systematic identification of regulons of transcriptional machineries

Otani

Mouncey

2022

Nat Commun

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Transcriptional regulation is a critical process to ensure expression of genes necessary for growth and survival in diverse environments. Transcription is mediated by multiple transcription factors including activators, repressors and sigma factors. Accurate computational prediction of the regulon of target genes for transcription factors is difficult and experimental identification is laborious and not scalable. Here, we demonstrate regulon identification by in vitro transcription-sequencing (RIViT-seq) that enables systematic identification of regulons of transcription factors by combining an in vitro transcription assay and RNA-sequencing. Using this technology, target genes of 11 sigma factors were identified in Streptomyces coelicolor A3(2). The RIViT-seq data expands the transcriptional regulatory network in this bacterium, discovering regulatory cascades and crosstalk between sigma factors. Implementation of RIViT-seq with other transcription factors and in other organisms will improve our understanding of transcriptional regulatory networks across biology.

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Section: Discussionmentioning

confidence: 99%

RIViT-seq enables systematic identification of regulons of transcriptional machineries

Otani

Mouncey

2022

Nat Commun

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“…In this organism, TopA is essential, and the decrease in its levels produces an increase in the supercoiling of DNA and the alteration of the expression of many genes (among them, the supercoiling hypersensitive clusters (SHCs) stand out), giving rise to a significant reduction in growth and blocked sporulation. The SatKR TCS was discovered because mutations in this system were able to suppress the blocking of sporulation associated with the high supercoiling of DNA by reduced levels of TopA [ 159 ].…”

Section: Two-component Systems Of Streptomyces Coelicolormentioning

confidence: 99%

“…When SatR levels are low, e.g., due to the absence of SatK, this RR inhibits supercooling-dependent SHC activation. However, when SatR levels are high through SatK activation, SHC transcription is induced independently of supercooling, resulting in blocked sporulation and growth [ 159 ].…”

Section: Two-component Systems Of Streptomyces Coelicolormentioning

confidence: 99%

“…This system was described in parallel with the SatKR TCS (previously discussed), as it is part of the supercoiling hypersensitive cluster (SHC) and is involved in the inhibition of sporulation [ 159 ]. It has been observed that the removal of SitKR can restore blocked sporulation due to DNA supercoiling conditions [ 159 ].…”

Section: Two-component Systems Of Streptomyces Coelicolormentioning

confidence: 99%

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Two-Component Systems of Streptomyces coelicolor: An Intricate Network to Be Unraveled

Nieta

Santamaría

Díaz

2022

IJMS

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Bacteria of the Streptomyces genus constitute an authentic biotech gold mine thanks to their ability to produce a myriad of compounds and enzymes of great interest at various clinical, agricultural, and industrial levels. Understanding the physiology of these organisms and revealing their regulatory mechanisms is essential for their manipulation and application. Two-component systems (TCSs) constitute the predominant signal transduction mechanism in prokaryotes, and can detect a multitude of external and internal stimuli and trigger the appropriate cellular responses for adapting to diverse environmental conditions. These global regulatory systems usually coordinate various biological processes for the maintenance of homeostasis and proper cell function. Here, we review the multiple TCSs described and characterized in Streptomyces coelicolor, one of the most studied and important model species within this bacterial group. TCSs are involved in all cellular processes; hence, unravelling the complex regulatory network they form is essential for their potential biotechnological application.

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An overview on the two-component systems of Streptomyces coelicolor

Jin

Hui

Lü

et al. 2023

World J Microbiol Biotechnol

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Global Chromosome Topology and the Two-Component Systems in Concerted Manner Regulate Transcription in Streptomyces

Cited by 3 publications

References 82 publications

RIViT-seq enables systematic identification of regulons of transcriptional machineries

RIViT-seq enables systematic identification of regulons of transcriptional machineries

Two-Component Systems of Streptomyces coelicolor: An Intricate Network to Be Unraveled

An overview on the two-component systems of Streptomyces coelicolor

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