Global DNA hypomethylation in breast carcinoma

Soares, Jorge Barbosa; Pinto, António E.; Cunha, Celso; André, Saudade; Barão, Isabel; Sousa, Joana; Cravo, Marília

doi:10.1002/(sici)1097-0142(19990101)85:1<112::aid-cncr16>3.3.co;2-k

Cited by 20 publications

(5 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results are consistent with the observations in the colon and stomach cancers, in which LINE-1 hypomethylation was present at an earlier stage of carcinogenesis [20,21]; however, the results differ from the observations that LINE-1 hypomethylation is considered a poor prognostic marker involved in the tumor progression and aggressive tumor behavior in HCC [27,37,38] and other type of cancers [42][43][44]. This difference may be in part due to the different type of markers of methylation, methodology, and criteria used in assessing hypomethylation of LINE-1.…”

Section: Discussionsupporting

confidence: 83%

Hypomethylation of Long Interspersed Nuclear Element-1 is Involved in the Early Tumorigenesis of Hepatocellular Carcinoma

Piao¹,

Wang²,

Zhang³

et al. 2014

J Interdiscipl Histopathol

View full text Add to dashboard Cite

Objective: Hypomethylation of long interspersed nucleotide elements 1 (LINE-1) promoter has been reported in many cancer types including pancreatic endocrine tumors, colon cancers, and stomach carcinomas. Hypomethylation of LINE-1 is also frequently observed in hepatocellular carcinoma (HCC). However, it still unknown how LINE-1 is involved in the hepatocarcinogenesis. Methods: The level of LINE-1 promoter methylation in 28 HCC was detected by a methylation specific polymerase chain reaction (MSP) at CpG site seven of LINE-1 promoter region (gene bank: X58075). A fractional allelic loss (FAL) of these tumors was evaluated by a combination of loss of heterozygosity with microsatellite markers D4S1545, D4S2920, D8S264, D8S1752, D16S498, D16S514, and p53. The associations between the level of LINE-1 hypomethylation status and the clinico-pathological parameters were finally observed. The clinicopathological parameters were included hepatitis B virus (HBV) status, cirrhosis, tumor size, tumor differentiation and FAL. Results: High level of hypomethylation of LINE-1 promoter was found both in the advanced tumors (>3 cm), (7/18, 39%) and in the early tumors (<3 cm), (6/10, 60%). Moreover, the high level of deoxyribonucleic acid hypomethylation at the LINE-1 was found in both poorly differentiated tumors (8/13, 61.5%) and well-differentiated tumors (5/15, 33.3%). The mean value of FAL in 28 HCCs was 0.535. All cases were divided according to FAL score: Low FAL (FAL < 0.535) and high FAL (FAL > 0.535). There were 41% (7/17) tumors with a high level of hypomethylation in the low FAL group and 54.5% (6/11) tumors with a high level of hypomethylation in the high FAL group. No associations between the level of hypomethylation of LINE-1 and HBV infection, age, sex, and cirrhosis were found. Conclusions: These results are strongly suggested that the hypomethylation of LINE-1 plays a role in the hepatocarcinogenesis; moreover, the hypomethylation of LINE-1 occurs not only in the progression of HCC, but also in the early stage of HCC tumorigenesis.

show abstract

Section: Discussionsupporting

confidence: 83%

Hypomethylation of Long Interspersed Nuclear Element-1 is Involved in the Early Tumorigenesis of Hepatocellular Carcinoma

Piao¹,

Wang²,

Zhang³

et al. 2014

J Interdiscipl Histopathol

View full text Add to dashboard Cite

show abstract

“…Results from liquid chromatography–electrospray ionization–tandem mass spectrometry (LC-ESI-MS/MS) show that the contents of 5-methyl-2′-deoxycytidine (5-mdC) in CTCs of breast cancer patients are lower than that in blood cells of healthy controls with matched ages and genders ( P = 0.002) (Figure d and Table S3). This is consistent with our previous result and matches well with literature showing that global DNA methylation declined in breast cancer tissues. − Taken together, it is clear that retrieved CTCs from degradable anti-EpCAM/HZnPNS can be successfully used for downstream molecular analysis, which will benefit personalized cancer therapy.…”

Section: Resultssupporting

confidence: 92%

Degradable Zinc-Phosphate-Based Hierarchical Nanosubstrates for Capture and Release of Circulating Tumor Cells

Guo

Xie

et al. 2016

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Circulating tumor cells (CTCs) play a significant role in cancer diagnosis and personalized therapy, and it is still a significant challenge to efficiently capture and gently release CTCs from clinical samples for downstream manipulation and molecular analysis. Many CTC devices incorporating various nanostructures have been developed for CTC isolation with sufficient capture efficiency, however, fabricating such nanostructured substrates often requires elaborate design and complicated procedures. Here we fabricate a degradable zinc-phosphate-based hierarchical nanosubstrate (HZnPNS), and we demonstrate its excellent CTC-capture performance along with effective cell-release capability for downstream molecular analysis. This transparent hierarchical architecture prepared by a low-temperature hydrothermal method, enables substantially enhanced capture efficiency and convenient imaging. Biocompatible sodium citrate could rapidly dissolve the architecture at room temperature, allowing that 88 ± 4% of captured cells are gently released with a high viability of 92 ± 1%. Furthermore, antiepithelial cell adhesion molecule antibody functionalized HZnPNS (anti-EpCAM/HZnPNS) was successfully applied to isolate CTCs from whole blood samples of cancer patients, as well as release CTCs for global DNA methylation analysis, indicating it will serve as a simple and reliable alternative platform for CTC detection.

show abstract

“…Methylation of both LINE-1 and ALU sequences was significantly higher in serum from normal controls than serum from breast cancer patients (p-values < 0.05 using Mann Whitney test), consistent with previous observations in similar studies investigating hypomethylation of repetitive elements in breast cancer. [21][22][23] Overall, LINE-1 methylation scores were higher than those for ALU regardless of normal or cancer samples, indicative of higher methylation density in LINE-1 sequences which is consistent with other studies investigating hypomethylation in a range of human cancers.…”

Section: This Journal Is ª the Royal Society Of Chemistry 2011supporting

confidence: 89%

Bisulfite-free analysis of 5MeC-binding proteins and locus-specific methylation density using a microparticle-based flow cytometry assay

Corrie

Sova

Feng

et al. 2011

Analyst

View full text Add to dashboard Cite

Global DNA hypomethylation in breast carcinoma

Cited by 20 publications

References 20 publications

Hypomethylation of Long Interspersed Nuclear Element-1 is Involved in the Early Tumorigenesis of Hepatocellular Carcinoma

Hypomethylation of Long Interspersed Nuclear Element-1 is Involved in the Early Tumorigenesis of Hepatocellular Carcinoma

Degradable Zinc-Phosphate-Based Hierarchical Nanosubstrates for Capture and Release of Circulating Tumor Cells

Bisulfite-free analysis of 5MeC-binding proteins and locus-specific methylation density using a microparticle-based flow cytometry assay

Contact Info

Product

Resources

About