Global DNA Methylation as a Potential Underlying Mechanism of Congenital Disease Development

Abstract

During the last decade, quantitative measurement of the methylation status in white blood cells (WBCs) has been used as a potential biomarker in a variety of diseases. Long interspersed nucleotide element-1 (LINE-1) has been used widely as a surrogate marker of global DNA methylation. Altered maternal DNA methylation is suggested to be an underlying mechanism in the trisomy 21 and the development of birth defects, including congenital heart defects (CHDs). The molecular mechanisms that underlie the epigenetic regulation of gene transcription are independent of DNA sequence, but they do depend on environmental stimuli, which are especially important in fetal development in utero environment. Folic acid deficiency and genetic variations of folate pathway genes, such as the methylenetetrahydrofolate reductase gene (MTHFR), are foremost among these maternal risk factors. Also, there are exogenous risk factors (cigarette smoking, alcohol intake, medication use, periconceptional maternal supplementation, body mass index, and dietary habits) with impact on maternal LINE-1 methylation. MTHFR C677T genotype/ diet and other environmental factors as significant predictors of LINE-1 DNA methylation in regard to congenital diseases will be discussed in the chapter.

Association of zinc level with DNA methylation and its consequences: A systematic review

“…The degree of global DNA methylation can be linked to functional implications, such as increased mutational events, genomic instability, or changed gene expression. Global hypomethylation within repetitive regions throughout the genome can lead to gene overexpression and transposable element activation, both of which can contribute to illness [ 52 ].…”

Methylation levels of LEP and POMC genes after intervention with dietary folate and hazelnut oil in the lipid profile of overweight women

“…Most scholars have reported associations between POMC gene variants and obesity phenotypes related to metabolic syndrome. However, recent studies do not make completely clear the mechanisms by which POMC methylation and lipid pro le changes occur [ 22,49,50] and the role of dietary fat intake in DNA methylation pro le changes [51][52][53].…”