Global Evolution of Pathogenic Bacteria With Extensive Use of Fluoroquinolone Agents

Füzi, M; Baño, Jesús Rodríguez; Tóth, Ákos

doi:10.3389/fmicb.2020.00271

Cited by 43 publications

(42 citation statements)

References 229 publications

(305 reference statements)

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“…Fluoroquinolone resistance has been indicated as a major facilitating factor in propagating the clonal expansion of major STs (e.g., ST15), particularly via low-fitness cost fluoroquinolone resistance-yielding point mutations in chromosomal genes (Tóth et al, 2014;Fuzi et al, 2017). It has been observed that strains of minor STs incapable of developing energetically favorable fluoroquinolone resistance mutations have a tendency to forego acquisition of more fitness costly resistance mechanisms, and remain fluoroquinolone susceptibility, but are on the other hand more capable of acquiring an extensive load of virulence genes (Fuzi et al, 2020). These observations are consistent with the results of the current study.…”

Section: Resultsmentioning

confidence: 99%

Hypervirulence Markers Among Non-ST11 Strains of Carbapenem- and Multidrug-Resistant Klebsiella pneumoniae Isolated From Patients With Bloodstream Infections

et al. 2020

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Multidrug-resistant Klebsiella pneumoniae and hypervirulent K. pneumoniae (hvKP) have traditionally been considered two individual populations; however, strains displaying both phenotypes have emerged during the recent decade. Understanding the genotypic and phenotypic basis of the convergence could be of clinical importance. In this study, we aimed to evaluate the pathogenicity associated with different combinations of genotypes (i.e., sequence types, virulence factors, and capsular serotypes) and phenotypes (i.e., hypermucoviscosity and drug susceptibility) in K. pneumoniae. A total of 11 K. pneumoniae isolates causing bloodstream infections were included in the study, and they were assigned to seven STs (ST23, ST15, ST268, ST660, ST86, ST65, and ST1660) and carried various K-loci (KL1, KL2, KL16, KL20, and KL24). Hypermucoviscosity was observed for six isolates. bla KPC−2 was detected in six carbapenem-resistant isolates, and the remaining ones were either multidrugresistant or resistant to two types of antibiotics. Aerobactin-and yersiniabactin-encoding genes were detected in all isolates. Although rmpA2 was detected in all isolates, most contained frameshift mutations (82%). Genes encoding salmochelin, RmpA, and PEG344 were detected in seven isolates. Colibactin-encoding genes were carried by six isolates. Discrepancies among measured virulence in Galleria mellonella and the serum-killing assay, and genotypes and phenotypes were detected. The results illustrate the complexity and difficulty with the current knowledge of hypervirulence to predict the phenotype by using genetic and phenotypic markers. Additionally, the emergence of carbapenem resistance in two isolates of KPC-2-producing hvKP of different sequence types emphasizes the urgency with which reliable clinical diagnostics for hvKP is needed.

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Section: Resultsmentioning

confidence: 99%

Hypervirulence Markers Among Non-ST11 Strains of Carbapenem- and Multidrug-Resistant Klebsiella pneumoniae Isolated From Patients With Bloodstream Infections

et al. 2020

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“…Non‐susceptibility to enrofloxacin might be one of the characteristics which confer to these isolates a selective advantage compared to the other isolates (Fuzi et al . 2020). Data on quinolone use in pigs in Canada are scarce, indeed, in the Canadian Integrated Program for Antimicrobial Resistance surveillance (CIPARS) report, fluoroquinolone use is surprisingly reported as inexistent, although these data concern only grower‐finisher pigs and do not provide information on use in farrowing barns or nurseries (Government of Canada 2016).…”

Section: Discussionmentioning

confidence: 99%

A new multidrug‐resistant enterotoxigenic Escherichia coli pulsed‐field gel electrophoresis cluster associated with enrofloxacin non‐susceptibility in diseased pigs

Lagarde

Vanier

Desmarais

et al. 2020

J. Appl. Microbiol.

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Aims To describe the temporal trends in Escherichia coli pathotypes and antimicrobial resistance detected in isolates from diseased‐pig cases submitted to the EcL from 2008 to 2016, in Quebec, Canada, and to investigate the presence of spatiotemporal and phylogenetic clusters. Methods and Results Detection of 12 genes coding for virulence factors in pathogenic E. coli in pigs by PCR and antimicrobial resistance standard disc diffusion assay were performed. Demographic and clinical data were entered in the Animal Pathogenic and Zoonotic E. coli (APZEC) database. ETEC:F4 was the most prevalent pathovirotype among the 3773 cases submitted. The LT:STb:F4 virotype was predominant until 2014, then was overtaken by the LT:STb:STa:F4 virotype. More than 90% of the ETEC:F4 isolates were multidrug resistant. A spatiotemporal cluster of LT:STb:STa:F4 isolates non‐susceptible to enrofloxacin was detected between 4/2015 and 9/2016. Pulsed‐field gel electrophoresis analysis of 137 ETEC:F4 isolates revealed the presence of a cluster composed mainly of LT:STb:STa:F4 isolates non‐susceptible to enrofloxacin. Conclusions The APZEC database was useful to highlight temporal trends in E. coli pathotypes. A high‐risk ETEC:F4 clone might disseminate in the pig population in Quebec since 2015. Significance and Impact of the Study Surveillance is crucial to identify new clones and develop control strategies.

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“…We noticed that among the 14 isolates with ParC S88P substitution, nine were clustered in the ESC R clade (clade A), suggesting that this substitution may be associated with ESC R . Intriguingly, it was reported that various MDR bacteria such as methicillin-resistant Staphylococcus aureus (MRSA), extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae , and ESBL-producing Escherichia coli were demonstrated to have been selected by favorable fitness balance associated with high-level resistance to fluoroquinolones, principally attained by the mutations of some serine residues in gyrA and parC/grlA ( Fuzi et al, 2017 , 2020 ). The association of the ParC S88P substitution and that of other QRDR serine replacements with fitness gain, the promotion of particular clades, and the acquisition of the MDR phenotype warrant further investigation.…”

Section: Discussionmentioning

confidence: 99%

Shanghai Neisseria gonorrhoeae Isolates Exhibit Resistance to Extended-Spectrum Cephalosporins and Clonal Distribution

et al. 2020

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The emergence of Neisseria gonorrhoeae strains with resistance (R) to extended-spectrum cephalosporins (ESCs R ) represents a public health threat of untreatable gonococcal infections. This study was designed to determine the prevalence and molecular mechanisms of ESC R of Shanghai N. gonorrhoeae isolates. A total of 366 N. gonorrhoeae isolates were collected in 2017 in Shanghai. Susceptibility to ceftriaxone (CRO), cefixime (CFM), azithromycin (AZM), ciprofloxacin (CIP), spectinomycin, penicillin, and tetracycline was determined using the agar dilution method. A subset of 124 isolates was subjected to phylogenetic analysis for nine antimicrobial resistance-associated genes, i.e., penA , porB , ponA , mtrR , 23S rRNA, gyrA , parC , 16S rRNA, and rpsE . Approximately 20.0% of the isolates exhibited CFM R [minimum inhibitory concentration (MIC) >0.125 mg/L], and 5.5% were CRO R (MIC > 0.125 mg/L). In total, 72.7% of ESC R isolates were clonal and associated with mosaic penA 10 and 60 alleles. Non-mosaic penA 18 allele and substitutions of PenA A501T, G542S, and PorB1b G213S/Y were observed in non-clonal ESC R . Approximately 6.8% of the isolates showed AZM MIC above the epidemiological cutoff (ECOFF, 1 mg/L), were associated with 23S rRNA A2059G mutation, and did not exhibit clonal distribution. Almost all isolates were CIP R (resistance to ciprofloxacin) and associated with GyrA-91/92 and ParC-85/86/87/88/89/91 alterations. Isolates with ParC S88P substitution were clustered into the ESC R clade. The Shanghai isolates exhibited a high level of ESC R and distinct resistant patterns.

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Global Evolution of Pathogenic Bacteria With Extensive Use of Fluoroquinolone Agents

Cited by 43 publications

References 229 publications

Hypervirulence Markers Among Non-ST11 Strains of Carbapenem- and Multidrug-Resistant Klebsiella pneumoniae Isolated From Patients With Bloodstream Infections

Hypervirulence Markers Among Non-ST11 Strains of Carbapenem- and Multidrug-Resistant Klebsiella pneumoniae Isolated From Patients With Bloodstream Infections

A new multidrug‐resistant enterotoxigenic Escherichia coli pulsed‐field gel electrophoresis cluster associated with enrofloxacin non‐susceptibility in diseased pigs

Shanghai Neisseria gonorrhoeae Isolates Exhibit Resistance to Extended-Spectrum Cephalosporins and Clonal Distribution

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