Global Gene Analysis of <i>Erigeron canadensis-</i>Treated TNF-α-, IL-4- and IL-1β-Stimulated A549 Human Epithelial Cells

Abstract

Background/Aims: This study was conducted to evaluate the anti-inflammatory mechanisms of Erigeron canadensis (EC) on the tumor necrosis factor-α (TNF-α)-, interleukin (IL)-4- and IL-1β-induced stimulation of A549 cells. Methods: In the present study, the anti-inflammatory effects of EC on TNF-α-, IL-4- and IL-1β-induced A549 cells were determined by analyzing eotaxin secretion using ELISA. In addition, the effects of ECon gene expression profiles in stimulated A549 cells were evaluated by microarray analysis. Results: Oligonucleotide microarray analysis revealed that inflammatory-related genes such as NOS1, NOS2A, IL-1β, IL-8 and CSF2 and cell adhesion-related genes such as SELE, MMP3, VCAM1, ICAM1, ITGA7 and ITGB2 were downregulated in EC-treated A549 cells that had been pretreated with TNF-α, IL-4 and IL-1β. In addition, significant decreases in Eotaxin, ICAM, VCAM and IL-8 gene expression were observed in EC-treated A549 cells. Conclusions: EC has an anti-inflammatory effect in stimulated A549 cells. Microarray-based genomic survey is a high-throughput approach that is suitable for the evaluation of gene expression in cell lines that have been treated with EC.

Lactobacillus frumentiimproves antioxidant capacityvianitric oxide synthase 1 in intestinal epithelial cells

“…We further validated the protein expressions, mRNA expressions, and enzyme activities of NOS1 in IPEC-J2 cells, and jejunal epithelial cells isolated from early-weaned piglets and germ-free mice. Although NOS1 is first identified in neuronal tissue, it also expresses in a variety of other cell types and tissues (46)(47)(48)(49). More and more studies have demonstrated that NOS1 is expressed in non-neuronal tissues, particularly epithelial cells (47)(48)(49).…”

“…Although NOS1 is first identified in neuronal tissue, it also expresses in a variety of other cell types and tissues (46)(47)(48)(49). More and more studies have demonstrated that NOS1 is expressed in non-neuronal tissues, particularly epithelial cells (47)(48)(49). Jarry et al (47) have demonstrated that NOS1 is strongly expressed in human kidney epithelial cells.…”

Pyranopyran-1,8-dione, an Active Compound from Vitices Fructus, Attenuates Cigarette-Smoke Induced Lung Inflammation in Mice

“…People, however, still long for cheaper and more effective alternative approaches to treat inflammation. Medicinal herbs have been used for treating inflammation related diseases, and they are often used to develop new medications [ 3 , 4 , 5 , 6 ]. It has been shown in a previous study that Fruit of Vitex trifolia subsp.…”

“…It has been shown in a previous study that Fruit of Vitex trifolia subsp. litoralis Steenis, Viticis Fructus (VF), has anti-inflammatory properties, which occur via inhibition of the inflammatory cytokines and the mitogen-activated protein kinase (MAPK)-signaling pathway [ 5 ]. VF is widely used in China and Korea for the treatment of pain, cold, headache, migraine, sore eyes, myalgia, and gastrointestinal infections such as bacterial dysentery and diarrhea [ 7 , 8 , 9 ].…”

Isolation and antifungal screening of endophytic fungi from Erigeron canadensis

“…Erigeron canadensis Linn., called horseweed widely distributed in China, is one of the herbaceous Chinese medicines used to treat intestinal diseases (Park et al, 2013;Wu et al, 2015). Modern pharmacological study showed that E. canadensis has strong bioactivties, including anti-inflammation (Sohn et al, 2009;Sung et al, 2014), anticoagulant and antiplatelet activity (Pawlacyk et al, 2011), inhibition of melanogenesis (Hong et al, 2008), cytotoxicity (Choi et al, 2008) and antifungal activity (Curini et al, 2003). Up to now, however, there is no report on biology and chemistry of endophytic microbes from this medicinal plant.…”