Global gene expression analysis during bovine oocyte in vitro maturation

Fair, Trudee; Carter, F.; Park, S.; Evans, A.C.O.; Lonergan, P.

doi:10.1016/j.theriogenology.2007.04.018

Cited by 80 publications

(79 citation statements)

References 30 publications

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“…4B). This study gave similar results to a previously published Affymetrix bovine chip analysis which identified 821 transcripts that were differentially expressed between in vivo and in vitro matured bovine oocytes (28).…”

Section: Comparison Of the Transcriptomic Profile In Vivo And In Vitrsupporting

confidence: 88%

Genome-wide expression profiling reveals distinct clusters of transcriptional regulation during bovine preimplantation development in vivo

Kues

Sudheer

Herrmann³

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

155

113

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Bovine embryos can be generated by in vitro fertilization or somatic nuclear transfer; however, these differ from their in vivo counterparts in many aspects and exhibit a higher proportion of developmental abnormalities. Here, we determined for the first time the transcriptomes of bovine metaphase II oocytes and all stages of preimplantation embryos developing in vivo up to the blastocyst using the Affymetrix GeneChip Bovine Genome Array which examines approximately 23,000 transcripts. The data show that bovine oocytes and embryos transcribed a significantly higher number of genes than somatic cells. Several hundred genes were transcribed well before the 8-cell stage, at which the major activation of the bovine genome expression occurs. Importantly, stage-specific expression patterns in 2-cell, 4-cell, and 8-cell stages, and in morulae and blastocysts, were detected, indicating dynamic changes in the embryonic transcriptome and in groups of transiently active genes. Pathway analysis revealed >120 biochemical pathways that are operative in early preimplantation bovine development. Significant differences were observed between the mRNA expression profiles of in vivo and in vitro matured oocytes, highlighting the need to include in vivo derived oocytes/embryos in studies evaluating assisted reproductive techniques. This study provides the first comprehensive analysis of gene expression and transcriptome dynamics of in vivo developing bovine embryos and will serve as a basis for improving assisted reproductive technology.

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Section: Comparison Of the Transcriptomic Profile In Vivo And In Vitrsupporting

confidence: 88%

Genome-wide expression profiling reveals distinct clusters of transcriptional regulation during bovine preimplantation development in vivo

Kues

Sudheer

Herrmann³

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

155

113

View full text Add to dashboard Cite

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“…On the basis of the GO annotation (of the identified known ESTs), it was determined that ,94% of transcripts detected in our library were involved in crucial molecular events associated with oocyte and preimplantation embryonic development in different mammalian species such as bovine (Dalbie's-Tran and Mermillod 2003;Fair et al, 2007;Ghanem et al, 2007;Salilew-Wondim et al, 2007), ovine (Baillet et al, 2008), human (Assou et al, 2006) and mice (Su et al, 2007). Identified genes were also classified as oocyte-specific genes involved in folliculogenesis, oogenesis, fertilization, zygotic genome activation and preimplantation embryo development (Table 3).…”

Section: Discussionmentioning

confidence: 99%

Identification of some unknown transcripts from SSH cDNA library of buffalo follicular oocytes

Rajput

Kumar

Roy

et al. 2013

Animal

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A buffalo oocyte-specific subtracted cDNA library was constructed to identify exclusively or preferentially oocyte-expressed genes. The library represented an enriched population of transcripts obtained from oocytes of diverse ovarian follicular origin and at different stages of in vitro maturation. A total of 1173 high-quality sequences of oocyte-specific genes were clustered into 645 unique sequences, out of which 65.76% were represented as singlets and 34.26% as contig expressed sequence tags (ESTs; clusters). Analysis of sequences revealed that 498 of these sequences were identified as a known sequence in mammalian species including buffalo, 103 as uncharacterized ESTs and 44 unknown sequences including 1 novel EST, so far not reported in any species. Gene ontology annotation classified these sequences into functional categories of cellular events and biological processes associated with oocyte competence. Expression status of the isolated unknown ESTs confirmed that many of these are expressed in oocytes exclusively and in others preferentially, some in excess of 80-fold greater in comparison with a variety of somatic tissues. The isolated novel EST was detected to be expressed exclusively in oocytes and testicular cells only. To our knowledge, this is the first report giving a detailed transcriptome account of oocyte-expressed genes in buffalo. This study will provide important information on the physiological control of oocyte development, as well as many questions yet to be addressed on the reproductive process of buffalo.

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“…There is differential expression of transcripts between in in vitro matured and immature bovine oocytes, which underlines the tight temporal control of protein synthesis required for oocyte maturation in preparation for subsequent fertilization and early embryonic development (Fair et al, 2007).…”

Section: Roles Of Mrnas In Ovarian Function and Oocyte Competencementioning

confidence: 99%

In search of the transcriptional blueprints of a competent oocyte

et al. 2017

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The oocyte undergoes a remarkably long and elaborated journey within the follicle before becoming fully equipped to sustain embryonic development. Its ability to support early embryonic development relies largely on the maternal transcripts accumulated during its growth and maturation. However, it is still not clear what transcriptome blueprint composes a competent oocyte. A number of extensive studies provided a detailed characterization of the mRNA molecules that are gradually accumulated in the oocyte cytoplasm. The detail of our knowledge has gradually increased through the years also thanks to the development and improvement of the analytical techniques. From realtime PCR analysis of single transcripts, to the whole transcriptome approach of gene arrays and new genereation sequencing, scientists accumulated an exponentially growing amount of new information. More recently, the discovery of non-coding RNAs revealed a new layer of complexity in the mechanisms that modulate gene expression at the mRNA level, in folliculogenesis and oogenesis. In particular, data are emerging on the potential role of microRNAs in controlling ovarian function, oocyte maturation and the oocyte-somatic cell cross talk. This review will try to summarize the vast amount of data currently available on the mRNAs and microRNAs associated with the ovarian function and to find their biological significance.

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Global gene expression analysis during bovine oocyte in vitro maturation

Cited by 80 publications

References 30 publications

Genome-wide expression profiling reveals distinct clusters of transcriptional regulation during bovine preimplantation development in vivo

Genome-wide expression profiling reveals distinct clusters of transcriptional regulation during bovine preimplantation development in vivo

Identification of some unknown transcripts from SSH cDNA library of buffalo follicular oocytes

In search of the transcriptional blueprints of a competent oocyte

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