Global Genomic Characterization of Salmonella enterica Serovar Telelkebir

Abstract

Non-typhoidal Salmonella (NTS) is a common cause for self-limiting gastroenteritis, representing a public health concern globally. NTS is one of the leading causes of foodborne illnesses in China; however, the invasive infection caused by NTS is largely underappreciated. Here, we reported an NTS invasive infection caused by an infrequently reported serovar Telelkebir (13,23:d:e,n,z15) strain FJ001 in China, which carries antimicrobial-resistant genes [fosA7 and aac(6′)-Iaa] and typhoid-toxin genes (cdtB, pltA, and pltB). By conducting the whole genomic sequencing, we also investigated the relatedness of this strain with an additional 120 global contextual Salmonella enterica serovar Telelkebir (S. Telelkebir) isolates, and assessed the antimicrobial-resistant determinants and key virulence factors using the available genomic dataset. Notably, all 121 (100%) of the S. Telelkebir strains possessed the typhoid toxin genes cdtB, pltA, and pltB, and 58.67% (71/121) of S. Telelkebir harbored antimicrobial-resistant gene fosaA7. The study by core genome multilocus sequence typing (cgMLST) and core single-nucleotide polymorphism (SNP)-based phylogenomic analysis demonstrated that the S. Telelkebir isolates from different sources and locations clustered together. This suggests that regular international travels might increase the likelihood of rapid and extensive transmissions of potentially pathogenic bacteria. For the first time, our study revealed the antimicrobial resistance, virulence patterns, and genetic diversity of the serovar S. Telelkebir isolate in humans and similar isolates over the world. The present study also suggests that genomic investigation can facilitate surveillance and could offer added knowledge of a previously unknown threat with the unique combination of virulent and antimicrobial-resistant determinants.

Antimicrobial Resistance Among Pathogens Causing Bloodstream Infections: A Multicenter Surveillance Report Over 20 Years (1998–2017)

“…More and more studies showed that surveillance genomic data could accelerate understanding of virulence and antimicrobial resistance. 29,30 Antibiotic susceptibility tests showed that the resistance rates of E. coli and K. pneumoniae to third-generation cefotaxime were significantly higher than those to ceftazidime, which is consistent with the 30-year data reported from CHINET in China. 3 Wang et al showed that CTX-M was the most important ESBL type in China and that cefotaxime resistance might be a sign of ESBL-producing bacterial strains.…”

“…More and more studies showed that surveillance genomic data could accelerate understanding of virulence and antimicrobial resistance. 29 , 30 …”

Genomic characterization of invasive typhoidal and non-typhoidal Salmonella in southwestern Nigeria

“…To the best our knowledge, this serovar has been reported just once on the African continent, in Mali (ENA accession number SRR8987873). However, it is more commonly reported in parts of Europe, China and USA (31). The expansion of atypical Salmonella serovars in invasive infections is associated with a high health burden (19,29).…”

“…S . Telelkebir has been reported a few times in Africa (as seen in Enterobase, [48]), and are more commonly reported in parts of Europe, China and USA [49]. The expansion of atypical Salmonella serovars in invasive infections is associated with a high health burden [17,36].…”

Antimicrobial Resistance Profiles and Genetic Typing of Salmonella Serovars from Chicken Embryos in China

“…Regarding Salmonella isolates, recent studies have demonstrated their resistance to many critical antimicrobial drugs, including polymyxin, β-lactams, and fluoroquinolones [15][16][17][18][19][20][21][22]. Indeed, these antimicrobial resistant strains can eventually be transmitted to humans through the food chain, causing severe infectious diseases [13,[23][24][25][26][27].…”