“…Recently, an evolutionarily conserved short-chain lysine acylation modification dubbed lysine 2-hydroxyisobutylation (K hib ) has been reported, which introduces a steric bulk with a mass shift of +86.03 Da ( Supplementary Figure 1A ) and neutralize the positive charge of lysine ( Dai et al, 2014 ; Xiao et al, 2015 ). It involves various biological functions including biosynthesis of amino acids, starch biosynthesis, carbon metabolism, glycolysis / gluconeogenesis and transcription ( Dai et al, 2014 ; Huang et al, 2017 , 2018a ; Li et al, 2017 ; Meng et al, 2017 ; Yu et al, 2017 ; Wu et al, 2018 ; Yin et al, 2019 ). For instance, the decrease of this modification on K281 of glycolytic enzyme ENO1 reduces its catalytic activity ( Huang et al, 2018b ).…”