Abstract. The net loss of soil organic carbon (SOC) from terrestrial ecosystems
is a likely consequence of global warming and may affect key soil
functions. The strongest changes in temperature are expected to occur at high
northern latitudes, with forest and tundra as prevailing land cover types.
However, specific soil responses to warming in different ecosystems are currently
understudied. In this study, we used a natural geothermal soil warming gradient (0–17.5 ∘C warming intensity) in an Icelandic spruce forest on Andosol to
assess changes in the SOC content between 0 and 10 cm (topsoil) and between 20 and 30 cm (subsoil)
after 10 years of soil warming. Five different SOC fractions were isolated,
and their redistribution and the amount of stable aggregates were
assessed to link SOC to changes in the soil structure. The results were compared to an
adjacent, previously investigated warmed grassland. Soil warming
depleted the SOC content in the forest soil by −2.7 g kg−1 ∘C−1 (−3.6 % ∘C−1) in the topsoil and −1.6 g kg−1 ∘C−1 (−4.5 % ∘C−1) in the
subsoil. The distribution of SOC in different fractions was significantly
altered, with particulate organic matter and SOC in sand and stable
aggregates being relatively depleted and SOC attached to silt and clay being
relatively enriched in warmed soils. The major reason for this shift was
aggregate breakdown: the topsoil aggregate mass proportion was reduced from
60.7±2.2 % in the unwarmed reference to 28.9±4.6 % in
the most warmed soil. Across both depths, the loss of one unit of SOC caused a
depletion of 4.5 units of aggregated soil, which strongly affected the bulk density
(an R2 value of 0.91 and p<0.001 when correlated with SOC, and an
R2 value of 0.51 and p<0.001 when correlated with soil mass in
stable aggregates). The proportion of water-extractable carbon increased
with decreasing aggregation, which might indicate an indirect protective
effect of aggregates larger than 63 µm on SOC. Topsoil changes in the
total SOC content and fraction distribution were more pronounced in the
forest than in the adjacent warmed grassland soils, due to higher and more
labile initial SOC. However, no ecosystem effect was observed on the warming response of the
subsoil SOC content and fraction distribution. Thus, whole profile
differences across ecosystems might be small. Changes in the soil structure upon warming should be studied more deeply and taken into consideration when interpreting or modelling biotic responses to warming.