Global Metabolic Profiling of Baculovirus Infection in Silkworm Hemolymph Shows the Importance of Amino-Acid Metabolism

Feng, Mei; Fei, Shigang; Xia, Junming; Zhang, Mengmeng; Wu, Hongyun; Swevers, Luc; Sun, Jingchen

doi:10.3390/v13050841

Cited by 16 publications

(8 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, it can be assumed that baculovirus infection has a major impact on the physiology of the cells and that the transcriptome analysis may not provide a clear picture of the characteristics of pro-hemocytes in healthy silkworms. Another transcriptome analysis revealed large changes in infected hemocytes regarding amino-acid, carbohydrate, nucleic acid and lipid metabolism, conform to the expectation that infected hemocytes are transformed into virion production factories (35). Because of this limitation, the analysis is focused on the 13 clusters that encompass the differentiated cell types of the silkworm hemocyte population (granulocytes, oenocytoids, plasmatocytes and spherulocytes).…”

Section: In Silico Analysis Of Predicted Marker Genes For Each Clustermentioning

confidence: 82%

Hemocyte Clusters Defined by scRNA-Seq in Bombyx mori: In Silico Analysis of Predicted Marker Genes and Implications for Potential Functional Roles

2022

Self Cite

View full text Add to dashboard Cite

Within the hemolymph, insect hemocytes constitute a heterogeneous population of macrophage-like cells that play important roles in innate immunity, homeostasis and development. Classification of hemocytes in different subtypes by size, morphology and biochemical or immunological markers has been difficult and only in Drosophila extensive genetic analysis allowed the construction of a coherent picture of hemocyte differentiation from pro-hemocytes to granulocytes, crystal cells and plasmatocytes. However, the advent of high-throughput single cell technologies, such as single cell RNA sequencing (scRNA-seq), is bound to have a high impact on the study of hemocytes subtypes and their phenotypes in other insects for which a sophisticated genetic toolbox is not available. Instead of averaging gene expression across all cells as occurs in bulk-RNA-seq, scRNA-seq allows high-throughput and specific visualization of the differentiation status of individual cells. With scRNA-seq, interesting cell types can be identified in heterogeneous populations and direct analysis of rare cell types is possible. Next to its ability to profile the transcriptomes of individual cells in tissue samples, scRNA-seq can be used to propose marker genes that are characteristic of different hemocyte subtypes and predict their functions. In this perspective, the identities of the different marker genes that were identified by scRNA-seq analysis to define 13 distinct cell clusters of hemocytes in larvae of the silkworm, Bombyx mori, are discussed in detail. The analysis confirms the broad division of hemocytes in granulocytes, plasmatocytes, oenocytoids and perhaps spherulocytes but also reveals considerable complexity at the molecular level and highly specialized functions. In addition, predicted hemocyte marker genes in Bombyx generally show only limited convergence with the genes that are considered characteristic for hemocyte subtypes in Drosophila.

show abstract

Section: In Silico Analysis Of Predicted Marker Genes For Each Clustermentioning

confidence: 82%

Hemocyte Clusters Defined by scRNA-Seq in Bombyx mori: In Silico Analysis of Predicted Marker Genes and Implications for Potential Functional Roles

2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…Baculovirus growth relies heavily on host resources. The utilization and distribution of energy in hosts can influence viral replication 32 . Glucose is the most critical energy source of insect cells in culture.…”

Section: Resultsmentioning

confidence: 99%

“…The utilization and distribution of energy in hosts can influence viral replication. 32 Glucose is the most critical energy source of insect cells in culture. Baculovirus uses the glucose of the host to provide energy for its replication through the tricarboxylic acid (TCA) cycle.…”

Section: Bacmid-4 × Pre-mir-34-egfp Affected Glucose Consumption and ...mentioning

confidence: 99%

microRNA‐34‐5p encoded by Spodoptera frugiperda regulates the replication and infection of Autographa californica multiple nucleopolyhedrovirus by targeting odv‐e66, ac78 and ie2

Jia

2022

Pest Management Science

View full text Add to dashboard Cite

BACKGROUND Spodoptera frugiperda is one of the significant migratory pests in the Global Alert issued by the Food and Agriculture Organization of the United Nations. As an insect‐specific microbial insecticide, baculovirus has been used to control various pests. MicroRNA‐34‐5p (miR‐34‐5p) is involved in regulating growth, reproduction and innate immunity to pathogens in insects, playing an essential role in host–virus interactions. In this study, we explored the critical function of miR‐34‐5p encoded by S. frugiperda in the anti‐Autographa californica multiple nucleopolyhedrovirus (AcMNPV), providing a reference for the design of a miR‐34‐5p target biopesticide against S. frugiperda and a theoretical basis for the wide application of microRNAs (miRNAs) in green pest control technology. RESULTS We focused on miR‐34‐5p identified as downregulated in Sf9 cells and S. frugiperda larvae infected by AcMNPV. The regulatory function of miR‐34‐5p in AcMNPV–S. frugiperda interactions was studied by transfecting synthetic mimics and inhibitors, and constructing recombinant bacmids with miR‐34‐5p overexpression. miR‐34‐5p inhibited the production of infectious budded virions at the cellular and insect levels, inhibited the replication of the viral DNA and glucose metabolism, and increased the transcription of the antimicrobial peptide gloverin. Furthermore, the virus genes odv‐e66, ac78 and ie2 were shown to be direct targets. CONCLUSION We systematically revealed the mechanism by which miR‐34‐5p is involved in the insect antiviral process. miR‐34‐5p inhibited the replication and infection of AcMNPV by directly targeting AcMNPV genes, especially ac78 and ie2. Our study provides a new direction and thinking for the prevention and green control of lepidopteran pests. © 2022 Society of Chemical Industry.

show abstract

“…Virus infection has significant impacts on host physiology, establishing optimal conditions for successful propagation (Lin et al, 2020). Viruses rely on host cell metabolism to provide the necessary energy and biosynthetic precursors for successful viral replication (Feng et al, 2021). We commonly think that the modulation of host physiology is for virus multiplication, but on the flip side, it can also be an aspect of host resistance.…”

Section: Discussionmentioning

confidence: 99%

“…miR-34-5p suppressed the glucose metabolism in host cells Baculovirus growth relies heavily on host resources. The utilization and distribution of energy in the host can influence viral replication (Feng et al, 2021). Glucose is the most critical energy source of insect cells in culture, and baculovirus uses the host's glucose to provide energy for its replication through the tricarboxylic acid (TCA) cycle (Kamen et al, 1996;Bernal et al, 2009;Lin et al, 2020).…”

Section: Validation Of Differential Expression Mirnas By Rt-qpcrmentioning

confidence: 99%

The microRNA pathway activation in insect cell model upon Autographa californica multiple nucleopolyhedrovirus infection

Jia¹,

Fu²

2023

Biocell

View full text Add to dashboard Cite

Background: The immune system of insects exerts fundamentally different antiviral mechanisms than mammals. MicroRNAs (miRNAs) play vital roles in developing insect antiviral immunity. MiRNAs expression profiles of insects changed significantly during baculovirus infection. Methods: Differential expression profiles of miRNAs in Spodoptera frugiperda were monitored by next-generation sequencing (NGS) and RT-qPCR during Autographa californica multiple nucleopolyhedrovirus (AcMNPV) infection. The transcription levels of genes were detected by RT-qPCR. The 50% tissue culture infective dose (TCID 50 ) endpoint dilution assay was used to determine the proliferation of progeny virus. Results: NGS revealed that 49 miRNAs were differentially expressed in Sf9 cells, and 10 of them were significantly up-or down-regulated. Though RT-qPCR analysis, we observed the similar trends for the expression patterns of significantly differentially expressed miRNAs from NGS. Moreover, the transcription levels of core genes, Exportin5, Dicer1, and Argonaute1, in miRNA biogenesis pathways were significantly increased after AcMNPV infection. For five selected miRNAs, miR-34-5p could regulate the proliferation of baculovirus progeny virus and energy metabolism. Conclusion: The miRNAs biogenesis pathway in Sf9 cells plays an important role and may be stimulated to resist AcMNPV infection. This work provides evidence for the molecular mechanism of baculovirus-insect interaction and offers novel ideas and directions for green pest control technology.

show abstract

Global Metabolic Profiling of Baculovirus Infection in Silkworm Hemolymph Shows the Importance of Amino-Acid Metabolism

Cited by 16 publications

References 35 publications

Hemocyte Clusters Defined by scRNA-Seq in Bombyx mori: In Silico Analysis of Predicted Marker Genes and Implications for Potential Functional Roles

Hemocyte Clusters Defined by scRNA-Seq in Bombyx mori: In Silico Analysis of Predicted Marker Genes and Implications for Potential Functional Roles

microRNA‐34‐5p encoded by Spodoptera frugiperda regulates the replication and infection of Autographa californica multiple nucleopolyhedrovirus by targeting odv‐e66, ac78 and ie2

The microRNA pathway activation in insect cell model upon Autographa californica multiple nucleopolyhedrovirus infection

Contact Info

Product

Resources

About