Global metabolic regulation analysis for Escherichia coli K12 based on protein expression by 2-dimensional electrophoresis and enzyme activity measurement

Peng, Lifeng; Shimizu, Kazuyuki

doi:10.1007/s00253-002-1202-6

Cited by 180 publications

(145 citation statements)

References 44 publications

Supporting

Mentioning

134

Contrasting

Order By: Relevance

“…1). Not surprisingly, this behavior correlates inversely with that of the TCA cycle, which becomes repressed during growth on D-glucose (6,320,342) and induced during growth on acetate (226,329,342).…”

Section: Recycling Coenzyme Amentioning

confidence: 95%

“…3) (6,160,420). In the absence of oxygen and under conditions that favor catabolite repression (e.g., excess glucose), E. coli cells do not induce the full TCA cycle (6,320,342). Instead, they operate a branched version, which forms succinyl-CoA by a reductive pathway and 2-ketoglutarate by an oxidative one (100,168,282,420).…”

Section: Limiting the Tricarboxylic Acid Cyclementioning

confidence: 99%

See 1 more Smart Citation

The Acetate Switch

Wolfe

2005

Microbiol Mol Biol Rev

1,080

1,274

View full text Add to dashboard Cite

To succeed, many cells must alternate between life-styles that permit rapid growth in the presence of abundant nutrients and ones that enhance survival in the absence of those nutrients. One such change in life-style, the “acetate switch,” occurs as cells deplete their environment of acetate-producing carbon sources and begin to rely on their ability to scavenge for acetate. This review explains why, when, and how cells excrete or dissimilate acetate. The central components of the “switch” (phosphotransacetylase [PTA], acetate kinase [ACK], and AMP-forming acetyl coenzyme A synthetase [AMP-ACS]) and the behavior of cells that lack these components are introduced. Acetyl phosphate (acetyl∼P), the high-energy intermediate of acetate dissimilation, is discussed, and conditions that influence its intracellular concentration are described. Evidence is provided that acetyl∼P influences cellular processes from organelle biogenesis to cell cycle regulation and from biofilm development to pathogenesis. The merits of each mechanism proposed to explain the interaction of acetyl∼P with two-component signal transduction pathways are addressed. A short list of enzymes that generate acetyl∼P by PTA-ACKA-independent mechanisms is introduced and discussed briefly. Attention is then directed to the mechanisms used by cells to “flip the switch,” the induction and activation of the acetate-scavenging AMP-ACS. First, evidence is presented that nucleoid proteins orchestrate a progression of distinct nucleoprotein complexes to ensure proper transcription of its gene. Next, the way in which cells regulate AMP-ACS activity through reversible acetylation is described. Finally, the “acetate switch” as it exists in selected eubacteria, archaea, and eukaryotes, including humans, is described

show abstract

Section: Recycling Coenzyme Amentioning

confidence: 95%

Section: Limiting the Tricarboxylic Acid Cyclementioning

confidence: 99%

The Acetate Switch

Wolfe

2005

Microbiol Mol Biol Rev

1,080

1,274

View full text Add to dashboard Cite

show abstract

“…Although studies have demonstrated that in vitro measured enzymatic activity do not obligatorily correlate with in vivo metabolic fluxes [21], enzyme activity can provide some information about metabolic changes. The lower activities of PFK and ICDH under oxidative condition than that in the control group indicated that PFK and ICDH were allosterically inhibited by higher metabolites concentrations in EMP and TCA under oxidative condition [22]. Metabolites measurement certified the enzyme activity result.…”

Section: Discussionmentioning

confidence: 99%

Suitable extracellular oxidoreduction potential inhibit

et al. 2014

View full text Add to dashboard Cite

Background: Polyketides, such as spinosad, are mainly synthesized in the stationary phase of the fermentation. The synthesis of these compounds requires many primary metabolites, such as acetyl-CoA, propinyl-CoA, NADPH, and succinyl-CoA. Their synthesis is also significantly influenced by NADH/NAD + . Rex is the sensor of NADH/NAD + redox state, whose structure is under the control of NADH/NAD + ratio. The structure of rex controls the expression of many NADH dehydrogenases genes and cytochrome bd genes. Intracellular redox state can be influenced by adding extracellular electron acceptor H 2 O 2 . The effect of extracellular oxidoreduction potential on spinosad production has not been studied. Although extracellular oxidoreduction potential is an important environment effect in polyketides production, it has always been overlooked. Thus, it is important to study the effect of extracellular oxidoreduction potential on Saccharopolyspora spinosa growth and spinosad production.

show abstract

“…It should be noted that the amount of protein is not always proportional to the protein activity, which in turn does not necessarily correlate with the corresponding metabolic reaction rate. However, it has been reported that protein abundance data obtained from proteome profiles appear to correlate to some extent with the enzyme activities in E. coli, with a few exceptions (231). Thus, it appears as though proteomics can be effectively used to identify candidates for successful metabolic engineering for improved bioproduct yield.…”

Section: Proteomics For Biotechnologymentioning

confidence: 99%

TheEscherichia coliProteome: Past, Present, and Future Prospects

Han¹,

Lee

2006

Microbiol Mol Biol Rev

166

130

View full text Add to dashboard Cite

SUMMARY Proteomics has emerged as an indispensable methodology for large-scale protein analysis in functional genomics. The Escherichia coli proteome has been extensively studied and is well defined in terms of biochemical, biological, and biotechnological data. Even before the entire E. coli proteome was fully elucidated, the largest available data set had been integrated to decipher regulatory circuits and metabolic pathways, providing valuable insights into global cellular physiology and the development of metabolic and cellular engineering strategies. With the recent advent of advanced proteomic technologies, the E. coli proteome has been used for the validation of new technologies and methodologies such as sample prefractionation, protein enrichment, two-dimensional gel electrophoresis, protein detection, mass spectrometry (MS), combinatorial assays with n-dimensional chromatographies and MS, and image analysis software. These important technologies will not only provide a great amount of additional information on the E. coli proteome but also synergistically contribute to other proteomic studies. Here, we review the past development and current status of E. coli proteome research in terms of its biological, biotechnological, and methodological significance and suggest future prospects.

show abstract

Global metabolic regulation analysis for Escherichia coli K12 based on protein expression by 2-dimensional electrophoresis and enzyme activity measurement

Cited by 180 publications

References 44 publications

The Acetate Switch

The Acetate Switch

Suitable extracellular oxidoreduction potential inhibit

TheEscherichia coliProteome: Past, Present, and Future Prospects

Contact Info

Product

Resources

About