2011
DOI: 10.1158/1535-7163.mct-10-0605
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Global microRNA Analysis of the NCI-60 Cancer Cell Panel

Abstract: MicroRNAs (miRNA) are a group of short noncoding RNAs that regulate gene expression at the posttranscriptional level. They are involved in many biological processes, including development, differentiation, apoptosis, and carcinogenesis. Because miRNAs may play a role in the initiation and progression of cancer, they comprise a novel class of promising diagnostic and prognostic molecular markers and potential drug targets. By applying an LNA-enhanced microarray platform, we studied the expression profiles of 95… Show more

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Cited by 72 publications
(69 citation statements)
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“…At present, it is unclear why miR-155 was not identified as differentially expressed in our microarray analysis. Because the LNA-based microarray platform used here has been used previously to identify miR-155 as differentially expressed in the NCI-60 cell line panel, 39 it is unlikely that the difference is because of platform related issues. We therefore speculate that cross-hybridization or presence of pre-miR-155 might have masked the signal of the mature miR-155 because the hybridization probes do not discriminate between mature and pre-miRNAs in the microarray platform.…”
Section: Discussionmentioning
confidence: 99%
“…At present, it is unclear why miR-155 was not identified as differentially expressed in our microarray analysis. Because the LNA-based microarray platform used here has been used previously to identify miR-155 as differentially expressed in the NCI-60 cell line panel, 39 it is unlikely that the difference is because of platform related issues. We therefore speculate that cross-hybridization or presence of pre-miR-155 might have masked the signal of the mature miR-155 because the hybridization probes do not discriminate between mature and pre-miRNAs in the microarray platform.…”
Section: Discussionmentioning
confidence: 99%
“…We analyzed the NCI-60, a cancer cell line panel containing 59 cell lines from nine tissue types, using our lectin microarray technology. This cell panel is a model for integrated analysis across multiple data types because of the availability of chemosensitivity profiles for these cells (21)(22)(23). The miRNA and mRNA profiles of the NCI-60 show the most coherent signatures for four tissue types: colon, leukemia, melanoma, and renal (22,23).…”
Section: Glycomic Analysis Of the Nci-60 Reveals Tissue Type-specificmentioning
confidence: 99%
“…This cell panel is a model for integrated analysis across multiple data types because of the availability of chemosensitivity profiles for these cells (21)(22)(23). The miRNA and mRNA profiles of the NCI-60 show the most coherent signatures for four tissue types: colon, leukemia, melanoma, and renal (22,23). We first profiled the glycome of cells from these four tissue types for integration with miRNA datasets and observed clear segregation by tissue of origin (SI Appendix, Fig.…”
Section: Glycomic Analysis Of the Nci-60 Reveals Tissue Type-specificmentioning
confidence: 99%
“…In this article, we address these questions by showing that the cell types in the widely representative NCI-60 panel of cancers [23][24][25][26] are amenable to isolation from blood by DEP. Recently, we modified this DEP-FFF approach to allow continuous-flow sorting of cells to achieve throughputs >10 6 nucleated cells/min, allowing 10 ml blood specimens to be processed in under an hour, 27,28 a method now being developed commercially.…”
mentioning
confidence: 99%