2016
DOI: 10.3389/fmicb.2016.00270
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Global MLST of Salmonella Typhi Revisited in Post-genomic Era: Genetic Conservation, Population Structure, and Comparative Genomics of Rare Sequence Types

Abstract: Typhoid fever, caused by Salmonella enterica serovar Typhi, remains an important public health burden in Southeast Asia and other endemic countries. Various genotyping methods have been applied to study the genetic variations of this human-restricted pathogen. Multilocus sequence typing (MLST) is one of the widely accepted methods, and recently, there is a growing interest in the re-application of MLST in the post-genomic era. In this study, we provide the global MLST distribution of S. Typhi utilizing both pu… Show more

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Cited by 29 publications
(27 citation statements)
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References 63 publications
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“…Sequences of seven housekeeping genes (aroC, dnaN, hemD, hisD, purE, sucA, and thrA) were compared with the available MLST database (http://mlst.warwick.ac.uk/mlst/dbs/Senterica) to get the allele number and sequence typing (ST) number for each isolate. Sequence information for newly assigned alleles and STs was deposited in the MLST database [14].…”
Section: Introductionmentioning
confidence: 99%
“…Sequences of seven housekeeping genes (aroC, dnaN, hemD, hisD, purE, sucA, and thrA) were compared with the available MLST database (http://mlst.warwick.ac.uk/mlst/dbs/Senterica) to get the allele number and sequence typing (ST) number for each isolate. Sequence information for newly assigned alleles and STs was deposited in the MLST database [14].…”
Section: Introductionmentioning
confidence: 99%
“…The generated sequence reads were trimmed, quality-checked, and assembled de novo using CLC Genomics Workbench 5.1 (CLC Bio, Denmark) as previously described [13]. A total of 28 and 11 contigs with the coverage of 98× and 101× were generated for LM115 and LM41, respectively.…”
Section: Methodsmentioning
confidence: 99%
“…Genomic DNA was extracted from a single colony of the pure bacterial culture. Potential contamination of the genomic library by foreign DNA was assessed using the CLC Genomics Workbench 5.1 (CLC Bio, Denmark) as previously described [13]. …”
Section: Quality Assurancementioning
confidence: 99%
“…These patterns indicated the considerable impact of genomic content on successful vs unsuccessful widespread dispersal among strains, as well as their respective evolution over time (Yap et al . ). Application of WGS has also helped to identify more localized geographical movement of serovars.…”
Section: Salmonella Identification and Epidemiology—wgsmentioning
confidence: 97%