Global profiling of lncRNAs-miRNAs-mRNAs reveals differential expression of coding genes and non-coding RNAs in the lung of beagle dogs at different stages of Toxocara canis infection

Zheng, Wen-Bin; Zou, Yang; He, Jun-Jun; Elsheikha, Hany M.; Li, Guohua; Hu, Min-Hua; Wang, Shuilian; Zhu, Xingquan

doi:10.1016/j.ijpara.2020.07.014

Cited by 15 publications

(32 citation statements)

References 86 publications

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“…Caspase-3 ( casp3 ) is a cysteine protease that plays a crucial role in apoptosis and inflammatory responses (Takashi et al, 2020 ) and osteogenic differentiation of bone marrow stromal stem cells (Miura et al, 2004 ). Our observation that the expression of casp3 in the bone marrow was increased 30.49 times at 24 hpi is consistent with a previous result detected in Beagle's lungs infected T. canis at the same time after infection (Zheng et al, 2021 ), suggesting that casp3 may play various roles during T. canis infection. Huntingtin interacting protein 1 (HIP1) is necessary to maintain cellularity in multiple tissue types, and the mutation of hip1 gene in mouse can cause abnormal hematopoiesis and spinal defects (Oravecz-Wilson et al, 2004 ).…”

Section: Discussionsupporting

confidence: 93%

“…As expected, no T. canis was found in puppies in the control groups. These results are consistent with those reported previously (Zheng et al, 2021). 2).…”

Section: Confirmation Of T Canis Infectionsupporting

confidence: 94%

“…Ten transcripts (five DEmRNAs and five DElncRNAs) at each of the four infection stages were randomly selected to validate the RNA-Seq results by quantitative real-time PCR (qRT-PCR) on LightCycler480 (Roche, Basel, Switzerland). Commercial kits, amplification conditions, and melting curve analysis for mRNA and lncRNA amplification were performed as described previously (Zheng et al, 2021 ). The expression levels of the selected DEmRNAs and DElncRNAs were normalized to that of the house-keeping genes.…”

Section: Methodsmentioning

confidence: 99%

“…Differences in neurotoxocarosis caused by T. canis and T. cati have been investigated by microarray analysis (Janecek et al, 2015). Additionally, metabolomics of serum and transcriptomics of lung tissue revealed several metabolic pathways and a considerable number of coding genes and long non-coding RNAs (lncRNAs), which mediate the interaction between T. canis and its canine host (Zheng et al, 2019(Zheng et al, , 2021. Despite these efforts, our understanding of the temporal changes of lncRNA, which are important transcriptional regulators of the immune and inflammatory responses to infection (Wang et al, 2015), in the bone marrow in dogs during T. canis infection is unknown.…”

Section: Introductionmentioning

confidence: 99%

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Toxocara canis Infection Alters lncRNA and mRNA Expression Profiles of Dog Bone Marrow

Zheng

Zou

Liu

et al. 2021

Front. Cell Dev. Biol.

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Bone marrow is the main hematopoietic organ that produces red blood cells, granulocytes, monocyte/macrophages, megakaryocytes, lymphocytes, and myeloid dendritic cells. Many of these cells play roles in the pathogenesis of Toxocara canis infection, and understanding how infection alters the dynamics of transcription regulation in bone marrow is therefore critical for deciphering the global changes in the dog transcriptional signatures during T. canis infection. In this study, long non-coding RNA (lncRNA) and messenger RNA (mRNA) expression profiles in the bone marrow of Beagle dogs infected with T. canis were determined at 12 h post-infection (hpi), 24 hpi, 96 hpi, and 36 days post-infection (dpi). RNA-sequencing and bioinformatics analysis identified 1,098, 984, 1,120, and 1,305 differentially expressed lncRNAs (DElncRNAs), and 196, 253, 223, and 328 differentially expressed mRNAs (DEmRNAs) at 12 h, 24 h, 96 h, and 36 days after infection, respectively. We also identified 29, 36, 38, and 68 DEmRNAs potentially cis-regulated by 44, 44, 51, and 80 DElncRNAs at 12 hpi, 24 hpi, 96 hpi, and 36 dpi, respectively. To validate the sequencing findings, qRT-PCR was performed on 10 randomly selected transcripts. Many altered genes were involved in the differentiation of bone marrow cells. GO of DElncRNAs and GO and KEGG pathway analyses of DEmRNAs revealed alterations in several signaling pathways, including pathways involved in energy metabolism, amino acid biosynthesis and metabolism, Wnt signaling pathway, Huntington's disease, HIF-1 signaling pathway, cGMP–PKG signaling pathway, dilated cardiomyopathy, and adrenergic signaling in cardiomyocytes. These findings revealed that bone marrow of T. canis-infected dogs exhibits distinct lncRNA and mRNA expression patterns compared to healthy control dogs. Our data provide novel insights into T. canis interaction with the definitive host and shed light on the significance of the non-coding portion of the dog genome in the pathogenesis of toxocariasis.

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Section: Discussionsupporting

confidence: 93%

“…As expected, no T. canis was found in puppies in the control groups. These results are consistent with those reported previously (Zheng et al, 2021). 2).…”

Section: Confirmation Of T Canis Infectionsupporting

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Toxocara canis Infection Alters lncRNA and mRNA Expression Profiles of Dog Bone Marrow

Zheng

Zou

Liu

et al. 2021

Front. Cell Dev. Biol.

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“…In this manner, the differentially regulated lncRNAs during E. necatrix infection might down-regulate host defense genes through recruitment of toll-like receptor and/or induce phosphorylation to activate inflammatory reactions (Fan et al, 2020). The in silico concomitant reduction of IL-21 and XLOC_237221 in dogs infected with T. canis requires functional analysis to substantiate humoral immune response and production of antibodies (Zheng et al, 2021) (Figure 3). T. gondii and T. canis are respectively entrenched and emerging zoonotic species while E. necatrix is of great veterinary importance.…”

Section: Activation Of Host-immune Genesmentioning

confidence: 99%

Functional Intricacy and Symmetry of Long Non-Coding RNAs in Parasitic Infections

Olajide

Olopade

Cai

2021

Front. Cell. Infect. Microbiol.

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RNAs are a class of molecules and the majority in eukaryotes are arbitrarily termed non- coding transcripts which are broadly classified as short and long non-coding RNAs. Recently, knowledge of the identification and functions of long non-coding RNAs have continued to accumulate and they are being recognized as important molecules that regulate parasite-host interface, parasite differentiation, host responses, and disease progression. Herein, we present and integrate the functions of host and parasite long non-coding RNAs during infections within the context of epigenetic re-programming and molecular crosstalk in the course of host-parasite interactions. Also, the modular range of parasite and host long non-coding RNAs in coordinated parasite developmental changes and host immune dynamic landscapes are discussed. We equally canvass the prospects of long non-coding RNAs in disease diagnosis and prognosis. Hindsight and suggestions are offered with the aim that it will bolster our understanding for future works on host and parasite long non-coding RNAs.

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Recent Advances in the Treatment of Parasitic Diseases: Current Status and Future

Kumar,

Deepika,

Sharda

et al. 2023

Natural Product Based Drug Discovery Against Human Parasites

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Global profiling of lncRNAs-miRNAs-mRNAs reveals differential expression of coding genes and non-coding RNAs in the lung of beagle dogs at different stages of Toxocara canis infection

Cited by 15 publications

References 86 publications

Toxocara canis Infection Alters lncRNA and mRNA Expression Profiles of Dog Bone Marrow

Toxocara canis Infection Alters lncRNA and mRNA Expression Profiles of Dog Bone Marrow

Functional Intricacy and Symmetry of Long Non-Coding RNAs in Parasitic Infections

Recent Advances in the Treatment of Parasitic Diseases: Current Status and Future

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