Global Survey of Antibiotic Resistance Genes in Air

Li, Jing; Cao, Junji; Zhu, Yong‐Guan; Chen, Qinglin; Shen, Fangxia; Wu, Yan; Xu, Siyu; Fan, Hongzhen; Da, Guillaume; Huang, Ru‐Jin; Wang, Jing; Jesus, Alma Lorelei de; Morawska, Lidia; Chan, Chak K.; Peccia, Jordan; Yao, Maosheng

doi:10.1021/acs.est.8b02204

Cited by 274 publications

(155 citation statements)

References 54 publications

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“…The lower prevalence of ARGs in the strains isolated from the biological part of the plant could be attributed to the type of processed waste and the design of the waste processing line. [32][33][34]. In our study, the isolates from the mechanical part of the MWMP also harbored genes encoding resistance to beta-lactams and chloramphenicol.…”

Section: Drug Resistance Genes In the Isolated Bacterial Strainsmentioning

confidence: 86%

Drug resistance in airborne bacteria isolated from waste management and wastewater treatment plants in Olsztyn

et al. 2019

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Wastewater treatment plants (WWTPs) and municipal waste management plants (MWMPs) emit bioaerosols containing potentially pathogenic biological components which post a threat for human health. Microbiological monitoring supports evaluations of the antibiotic resistance (AR) of airborne microorganisms and the relevant health risks. The aim of this study was to analyze the microbiological quality of air sampled in a WWTP and MWMP in Olsztyn based on total bacterial counts, the presence of bacteria resistant to three antibiotic classes (beta-lactams, tetracyclines and chloramphenicol) and genes encoding resistance to these antibiotics (blaTEM, blaSHV, blaCMY-2, blaAmpC, tet(M), tet(A), tet(X), tet(B), cmlA, floR, fexA, fexB and catA1 ). Bacterial counts were higher in air samples collected from the MWMP (~104 CFU/m3) than from the WWTP (101–103 CFU/m3). A similar trend was noted in the counts of antibiotic resistant bacteria (ARB). The abundance of ARB did not exceed 1.7 x 102 CFU/m3 in WWTP samples, but was higher at up to 4.2 x 103 CFU/m3 in MWMP samples. Bacteria resistant to doxycycline were least prevalent in the analyzed ARB. In the group of 49 tested bacterial strains, 44 harbored at least one of the analyzed antibiotic resistance genes (ARGs). A comparison of ARGs in all bacterial strains isolated from WWTP and MWMP air samples revealed the highest diversity and prevalence of ARGs in the samples collected in the mechanical segment of the waste processing line in MWMP and the biological segment of the wastewater processing line in WWTP. The results of this study point to high microbiological contamination of air in MWMPs and WWTPs which are reservoirs of ARB and ARGs and potential sources of AR.

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Section: Drug Resistance Genes In the Isolated Bacterial Strainsmentioning

confidence: 86%

Drug resistance in airborne bacteria isolated from waste management and wastewater treatment plants in Olsztyn

et al. 2019

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“…Particles from different sources had different particle mobility graphs as shown in the figure under the same iSCAPE operating conditons (3.17 kv/ cm for 2 min). The differences observed for different cities via the iSCAPE were likely due to the different components such as bacteria and metals, and the size distrbutions of their PMs (5, 16,18) . For example, air samples from Zurich were shown to have higher fraction of nanoscale particles than those from Beijing (5) .…”

Section: Resultsmentioning

confidence: 99%

“…To test the iSCAPE system, we used atmospheric samples previously collected using automobile air conditioning filters for Beijing, Zurich, and San Francisco (16) . Here, we also collected Beijing’s soil and road dust samples for testing the system.…”

Section: Methodsmentioning

confidence: 99%

Fine Sieving of Collected Atmospheric Particles using Oil Electrophoresis (iSCAPE)

Yao

2020

Preprint

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It is rather challenging to separate atmospheric particles from nano-to micro-metre mixed in a sample. Here, a system named iSCAPE was invented to efficiently sieve particles out from a mixture by employing an electrostatic field and a non-conductive mineral oil. Tests with atmospheric particles of different cities as well as soil and road dust samples demonstrated that the iSCAPEd particles under different operating conditions moved rapidly with different velocities and both directions. Particles of different sources such as ambient air, soil or road were shown to have different polarity-charged particle fractions, and exhibited clearly different particle electrical mobility graphs after the iSCAPE sieving from seconds to minutes. Data also revealed that after the sieving some particles were enriched at specific mobility ranges. Bacterial ATP measurements implied that the iSCAPE can be also used to efficiently separate bacteria of different sizes and charge polarity. Experimental data here suggest that the iSCAPE sieving strongly replies on the electrostatic field strength, mineral oil viscosity and the run time. In theory, the iSCAPE system can be used to extract any desired targets from a complex sample, thus opening up many outstanding opportunities for environmental, biomedical and life science fields.TOC

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“…Thus, bioaerosols larger than 5 µm can only affect people located at a close distance while smaller particles (1 µm to 5 µm) can be aero-transported and affect people located at larger distances. Despite growing evidence demonstrating microorganisms [1] or even virulence factors such as antibiotic resistance genes [5] can be effectively transmitted through air, the contribution of airborne transmission in hospital infections has received less attention.…”

Section: Introductionmentioning

confidence: 99%

QuEChER method for air microbiological monitoring in hospital environments

Busso

Herrera

Tames

et al. 2020

J Infect Dev Ctries

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Introduction: Nosocomial pathogens have become a priority issue for public health, since they are responsible for increased morbidity and mortality in hospitalized patients and the development of multi-resistant microorganisms, as well. Recent studies found strong evidence that airborne transmission plays a key role in many nosocomial infections. Thus, we aim to develop a QuEChER methodology for the characterization of airborne microbial levels, analyzing potential variables that modify the air microbiological load. Methodology: Particulate matter levels and suspended and settled bioaerosols were determined simultaneously employing optical sensors, Harvard impactors and settle plates, respectively. Environmental variables were also measured at different sites during different working shifts and seasons. Results: We found a straightforward relationship between airborne particles, air exchange rates, and people influx. Levels of suspended microorganisms were related to fine particulate matter concentration, CO2 and ambient temperature. A positive linear relationship (R2 = 0.9356) was also found between fine particulate matter and CO2 levels and air microbial load. Conclusion: The QuEChER methodology is an effective methodology that could be used to improve the surveillance of nosocomial pathogens in developing countries hospitals where air quality is scarcely controlled.

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Global Survey of Antibiotic Resistance Genes in Air

Cited by 274 publications

References 54 publications

Drug resistance in airborne bacteria isolated from waste management and wastewater treatment plants in Olsztyn

Drug resistance in airborne bacteria isolated from waste management and wastewater treatment plants in Olsztyn

Fine Sieving of Collected Atmospheric Particles using Oil Electrophoresis (iSCAPE)

QuEChER method for air microbiological monitoring in hospital environments

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