Global urinary metabolic profiling of the osteonecrosis of the femoral head based on UPLC–QTOF/MS

Ye, Gang; Zhao, Gang; Zhang, Jian; Gao, Sichuan; Chen, Tingmei; Ding, Shijia; Zhu, Yun

doi:10.1007/s11306-019-1491-8

Cited by 15 publications

(8 citation statements)

References 64 publications

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“…Furthermore, l -serine and cystine levels significantly increased in the model group and slightly decreased in the treatment group. Gang et al showed that l -serine increased in osteonecrosis, and l -serine promoted the formation of osteoclasts, thereby inducing bone resorption, which might be related to the effect of l -serine on RANKL signal transduction [ 37 ]. Cystine has the effect of negatively regulating Runx2, thereby affecting bone regeneration, a finding which is supported by our immunohistochemistry results [ 38 ].…”

Section: Resultsmentioning

confidence: 99%

“…Many reports have confirmed that metabolic pathway disorders associated with pyrimidine metabolism, cysteine and methionine metabolism, and galactose metabolism are related to steroid therapy and osteonecrosis [ 37 , 41 ]. Additionally, members of the ABC transporter family are involved in various cell regulation processes, such as lipid transport and iron metabolism.…”

Section: Resultsmentioning

confidence: 99%

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Biofunctionalized composite scaffold to potentiate osteoconduction, angiogenesis, and favorable metabolic microenvironment for osteonecrosis therapy

Zhu

Jiang

Zhang

et al. 2022

Bioactive Materials

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Biofunctionalized composite scaffold to potentiate osteoconduction, angiogenesis, and favorable metabolic microenvironment for osteonecrosis therapy

Zhu

Jiang

Zhang

et al. 2022

Bioactive Materials

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“…Osteonecrosis, an idiopathic process affecting predominately males and involving bilateral hips in 80% of cases, has known multifactorial associations, including irradiation, trauma, hematologic conditions, marrow-replacing diseases, sickle cell disease, alcoholism, and hypercoagulable states; therefore, the implicated molecular mechanisms may be as varied as the causes. Yang et al performed the urinary metabolomic profiling of a patient cohort with confirmed femoral osteonecrosis (with varied histories, including alcohol consumption, femoral neck or intertrochanteric fracture, corticosteroid therapy, and idiopathic osteonecrosis) and found increased urea, deoxycholic acid, and phosphatidylethanolamine [36]. In a plasma-based metabolomic analysis of patients with confirmed osteonecrosis, Liu et al reported alterations in lipid metabolism, nucleotide metabolism, and cysteine metabolism; lipid metabolism was primarily downregulated [37].…”

Section: Osteonecrosis/avascular Necrosismentioning

confidence: 99%

Metabolomic Profiling in the Characterization of Degenerative Bone and Joint Diseases

et al. 2020

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Osteoarthritis and inflammatory arthropathies are a cause of significant morbidity globally. New research elucidating the metabolic derangements associated with a variety of bone and joint disorders implicates various local and systemic metabolites, which further elucidate the underlying molecular mechanisms associated with these destructive disease processes. In osteoarthritis, atty acid metabolism has been implicated in disease development, both locally and systemically. Several series of rheumatoid arthritis patients have demonstrated overlapping trends related to histidine and glyceric acid, while other series showed similar results of increased cholesterol and glutamic acid. Studies comparing osteoarthritis and rheumatoid arthritis reported elevated gluconic acid and glycolytic- and tricarboxylic acid-related substrates in patients with osteoarthritis, while lysosphingolipids and cardiolipins were elevated only in patients with rheumatoid arthritis. Other bone and joint disorders, including osteonecrosis, intervertebral disc degeneration, and osteoporosis, also showed significant alterations in metabolic processes. The identification of the molecular mechanisms of osteoarthritis and inflammatory arthropathies via metabolomics-based workflows may allow for the development of new therapeutic targets to improve the quality of life in these patient populations.

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“…Mass spectrum conditions: Mass spectrometric detection of metabolites was performed on Q Exactive Focus (Thermo Fisher Scientific, USA) with an ESI ion source and simultaneous MS1 and MS/MS (Full MS-ddMS2 mode, data-dependent MS/MS) acquisition. The parameters were as follows: sheath gas pressure, 30 arb; aux gas flow, 10 arb; spray voltage, 3.50 kV and -2.50 kV for ESI ( +) and ESI (-), respectively; capillary temperature, 325 ℃; MS1 range, m/z 100-1000; MS1 resolving power, 70,000 FWHM; the number of data-dependent scans per cycle, 3; MS/MS resolving power, 17,500 FWHM; normalized collision energy, 30 eV; dynamic exclusion time, automatic [41].…”

Section: Lc-ms Analysismentioning

confidence: 99%

Determining the changes in metabolites of Dendrobium officinale juice fermented with starter cultures containing Saccharomycopsis fibuligera FBKL2.8DCJS1 and Lactobacillus paracasei FBKL1.3028 through untargeted metabolomics

et al. 2023

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Background The present study aimed to investigate the changes in volatile components and metabolites of Dendrobium officinale (D. officinale) juice fermented with starter cultures containing Saccharomycopsis fibuligera and Lactobacillus paracasei at 28 ℃ for 15 days and post-ripened at 4 ℃ for 30 days using untargeted metabolomics of liquid chromatography-mass spectrometry (LC–MS) and headspace solid-phase microextraction-gas chromatography (HS–SPME–GC–MS) before and after fermentation. Results The results showed that the alcohol contents in the S. fibuligera group before fermentation and after fermentation were 444.806 ± 10.310 μg/mL and 510.999 ± 38.431 μg/mL, respectively. Furthermore, the alcohol content in the fermentation broth group inoculated with the co-culture of L. paracasei + S. fibuligera was 504.758 ± 77.914 μg/mL, containing a significant amount of 3-Methyl-1-butanol, Linalool, Phenylethyl alcohol, and 2-Methyl-1-propanol. Moreover, the Ethyl L (-)-lactate content was higher in the co-culture of L. paracasei + S. fibuligera group (7.718 ± 6.668 μg/mL) than in the L. paracasei (2.798 ± 0.443 μg/mL) and S. fibuligera monoculture groups (0 μg/mL). The co-culture of L. paracasei + S. fibuligera significantly promoted the metabolic production of ethyl L (-)-lactate in D. officinale juice. The differential metabolites screened after fermentation mainly included alcohols, organic acids, amino acids, nucleic acids, and their derivatives. Twenty-three metabolites, including 11 types of acids, were significantly up-regulated in the ten key metabolic pathways of the co-culture group. Furthermore, the metabolic pathways, such as pentose and glucuronate interconversions, the biosynthesis of alkaloids derived from terpenoid and polyketide, and aminobenzoate degradation were significantly up-regulated in the co-culture group. These three metabolic pathways facilitate the synthesis of bioactive substances, such as terpenoids, polyketides, and phenols, and enrich the flavor composition of D. officinale juice. Conclusions These results demonstrate that the co-culture of L. paracasei + S. fibuligera can promote the flavor harmonization of fermented products. Therefore, this study provides a theoretical basis for analyzing the flavor of D. officinale juice and the functional investigation of fermentation metabolites.

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Global urinary metabolic profiling of the osteonecrosis of the femoral head based on UPLC–QTOF/MS

Cited by 15 publications

References 64 publications

Biofunctionalized composite scaffold to potentiate osteoconduction, angiogenesis, and favorable metabolic microenvironment for osteonecrosis therapy

Biofunctionalized composite scaffold to potentiate osteoconduction, angiogenesis, and favorable metabolic microenvironment for osteonecrosis therapy

Metabolomic Profiling in the Characterization of Degenerative Bone and Joint Diseases

Determining the changes in metabolites of Dendrobium officinale juice fermented with starter cultures containing Saccharomycopsis fibuligera FBKL2.8DCJS1 and Lactobacillus paracasei FBKL1.3028 through untargeted metabolomics

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