Globin synthesis in hybrid cells constructed by transplantation of dormant avian erythrocyte nuclei into enucleated fibroblasts.

Bruno, J; Reich, N; Lucas, Joseph J.

doi:10.1128/mcb.1.12.1163

Cited by 15 publications

(9 citation statements)

References 34 publications

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“…This time difference is in accordance with nuclear transplantation experiments (Bruno et al, 1981) which showed that avian erythrocyte nuclei, transplanted into fibroblast cells, expressed ~-globin after several hours but that [~-globin expression could not be detected even after 18 hours.…”

Section: Developmental Differences In the Expression Patterns Of Globsupporting

confidence: 89%

Differences in erythropoiesis in normal chicken and quail embryos

1993

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Using antibodies against the fetal and adult forms of alpha- and beta-globin, it has been shown that erythropoiesis in the para-aortic foci (PAF) constitutes a major species-specific difference between chicken and quail embryos. In quail embryos, para-aortic foci are rare, small and rather heterogeneous with regard to their erythropoietic and haemopoietic cell composition. In contrast, the PAFs in chicken embryos are abundant and consist of large numbers of erythropoietic cells. In both species a time difference (approximately 1 day) is observed between the first expression of the fetal alpha- and beta-globin and the adult alpha- and beta-globin in erythropoietic cells. Adult erythropoiesis in both species can be detected first in the stalk of the yolk sac; this is similar to the situation in mammalian and amphibian species. From this time onward the number of circulating adult erythrocytes increases steadily. Whereas in chicken, large intraembryonic foci that can serve as sources for these adult cells arise concomitantly, no such foci can be detected in quail embryos, suggesting that the quail yolk sac is a major source for these adult red blood cells.

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Section: Developmental Differences In the Expression Patterns Of Globsupporting

confidence: 89%

Differences in erythropoiesis in normal chicken and quail embryos

1993

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“…As shown in Figure 2, the general pattern of :j5Smethionine-containing polypeptides was quite similar for erythroblasts which were grown at 36°C and for reticulocytes from 42°C cultures. However, a number of differences were observed of which the most striking was the appearance at 42°C of a major, low molecular weight polypeptide with the relative migration in NEPHGE-SDS gel electrophoresis expected for chicken 6-globin (Bruno et al, 1981). Although some a-globin is apparently being produced in these cells at the permissive temperature, its synthesis is dramatically stimulated following a shift to the restrictive temperature.…”

Section: Protein Synthesis In Differentiating Tsaev-infected Erythroimentioning

confidence: 98%

Protein synthesis in differentiating normal and leukemic erythroid cells

Adkins

Beug

Graf

1985

Journal Cellular Physiology

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Erythroleukemic cells transformed by the AEV or S13 strains of avian erythroblastosis virus differentiate in vitro either spontaneously (S13) or following a temperature induction (temperature-sensitive mutants of AEV). To study differentiation in these cells at the molecular level, homogeneous fractions of maturing cells at discrete stages of differentiation were prepared by Percoll density-gradient centrifugation. This method was also used for the fractionation of differentiating normal erythroid cells separated from total bone marrow by an immunological "panning" technique. Total protein synthesis in these cells was then analyzed by two-dimensional gel electrophoresis. The expression of several proteins was altered in differentiating leukemic cells but not in their normal counterparts. However, in general, the normal and leukemic cells from comparable stages of maturity showed closely related protein synthetic patterns. Similar early and late changes in the synthesis of a number of polypeptides were detected during maturation from early erythroid precursors to terminally differentiated erythrocytes. Further, the leukemic as well as the normal cells appeared to undergo a major switch in total protein synthetic pattern during late differentiation. These results demonstrate that normal and erythroleukemic cells differentiate along similar pathways.

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“…Two-dimensional gel electrophoresis. Samples were prepared, and gel electrophoresis was performed by using procedures based on those described by O'Farrell (16), O'Farrell et al (17), and Garrels (6 (4). First-dimension isoelectric focusing gels were run as described by O'Farrell (16), except that the gels were 170 mm in length and 1.8 mm in diameter.…”

mentioning

confidence: 99%

“…Focusing was performed for 7,650 V-h with an additional final hour at 800 V. Only the most acidic 120 mm of the gel was applied to the seconddimension slab gel (7). Second-dimension gels were run as described by Bruno et al (4). A variety of purified marker proteins were used in the identification of spots on the gel patterns.…”

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confidence: 99%

Programmed macromolecular synthesis in regenerating karyoplasts.

White¹,

Bruno²,

Lucas³

1983

Mol. Cell. Biol.

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Conditions for the preparation, purification, and maintenance of karyoplasts which could regenerate to reform whole viable cells were defined. Results of biochemical analyses of such karyoplasts at various times during regeneration indicated that a reproducible biosynthetic program was followed. Thus, an examination of the polypeptides made during regeneration by two-dimensional gel electrophoresis showed that the pattern of radiolabeled polypeptides synthesized at each time studied was specific and was significantly different from that observed at other times during regeneration. Polypeptides associated with three major cellular fractions-nuclear, cytoskeletal-microtrabecular, and solublewere among the most dramatically regulated molecules. Other polypeptides, such as the major components of microfilaments and intermediate filaments, were synthesized at relatively constant rates and were assembled into structures throughout regeneration. Likewise, microtubules appeared to be reformed throughout regeneration, even in the absence of identifiable centriole-associated organizing centers. Finally, analysis of DNA synthesis by autoradiography showed that, even when prepared from whole cells synchronized at the Gl/S interface, karyoplasts could not begin making DNA until they had regenerated an almost complete complement of cytoplasm.Cytochalasin-induced enucleation of mammalian cells produces primarily two types of cellular fragments: cytoplasts, or enucleated cells, and karyoplasts, or nuclei surrounded by thin shells of cytoplasm enclosed within outer cell membranes (5,18,21

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Globin synthesis in hybrid cells constructed by transplantation of dormant avian erythrocyte nuclei into enucleated fibroblasts.

Abstract: The polypeptides synthesized by mature embryonic erythrocytes prepared from the peripheral blood of 14-to 15-day-old chicken embryos were analyzed by two-dimensional gel electrophoresis. Fewer

Cited by 15 publications

References 34 publications

Differences in erythropoiesis in normal chicken and quail embryos

Differences in erythropoiesis in normal chicken and quail embryos

Protein synthesis in differentiating normal and leukemic erythroid cells

Programmed macromolecular synthesis in regenerating karyoplasts.

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