Glomerular antigens in severe hereditary nephrosis

Holthöfer, Harry; Haltia, Anni; Jalanko, Hannu; Holmberg, Christer; Solin, Marja-Liisa

doi:10.1111/j.1699-0463.1995.tb01441.x

Cited by 4 publications

(6 citation statements)

References 15 publications

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“…Other criteria for the identification of the cobblestone‐like podocytes used in the present study were the barely detectable immunological reaction of desmin (the marker of mesangial cells (Holthofer et al, 1995; Cluzeaud et al, 1996)), and the presence of Wilms' tumor protein (the marker of podocytes (Mundlos et al, 1993)), which is absent from mesangial cells. Double‐labeling in glomerular cultures of desmin and Wilms' tumor protein (Fig.…”

Section: Resultsmentioning

confidence: 98%

Glial fibrillary acidic protein‐positive cells of the kidney are capable of raising a protective biochemical barrier similar to astrocytes: Expression of metallothionein in podocytes

et al. 2002

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Blood-tissue exchange and homeostasis within the organs depend on various interactions between endothelial and perivascular cells (Buniatian, 2001). Podocytes possess anatomical and cellular features intermediate between those of astrocytes and hepatic stellate cells (HSCs). Podocytes, like HSCs, are associated with fenestrated capillaries and, similar to astrocytes, interact with the capillaries via the basement membrane and participate in permeability-limiting ultrafiltration. The fact that podocytes come in direct contact with xenobiotics prompted us to investigate whether they express metallothionein (MT), an anticytotoxic system characteristic of astrocytes. In comparative studies, cryosections of 1-and 3-month-old rat kidney and adult rat brain, as well as podocytes and astrocytes from early and prolonged primary cultures of glomerular explants and newborn rat brain, respectively, were investigated. The cells were double-labeled with antiserum against glial fibrillary acidic protein (GFAP) and monoclonal antibody (MAb) against the lysine-containing epitope of Cd/Zn-MT-I (MAb MT) or MAb against alpha-actin. In kidney sections, MT immunoreactivity was detected in GFAP-positive glomerular cells and in interstitial fibroblasts. The pattern of staining for MT and GFAP in glomerular cells was similar to that of astrocytes in vivo. In glomerular cell cultures, MT was expressed in cobblestone-like podocytes which contained Wilms' tumor protein and lacked desmin. MT was upregulated at later culture periods, during which podocytes acquired features typical of undifferentiated astrocytes. This study hints at the existence of common regulatory mechanisms of blood-tissue interactions by neural and non-neural perivascular cells. These mechanisms appear to be used in an organ-specific manner.

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Section: Resultsmentioning

confidence: 98%

Glial fibrillary acidic protein‐positive cells of the kidney are capable of raising a protective biochemical barrier similar to astrocytes: Expression of metallothionein in podocytes

et al. 2002

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“…In our experiments, MC did not express the CD49a molecule on the surface. Some data in the literature indicate that this molecule is highly expressed on the mesangial cell surface, while others showed only a weak and focal expression, or the presence of mRNA only (8, 22, 26, 27). One possible explanation for the lack of CD49a presence is the long culture period in our study, as has been discussed previously in the literature (28).…”

Section: Discussionmentioning

confidence: 99%

Surface antigens of human mesangial cells: impact of growth surface or IL‐1α

et al. 2002

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The interactions of mesangial cells (MC) with their environment are important events in glomerular physiology and pathology, yet a detailed characterization of the MC-surface antigens mediating these interactions is still lacking. In this study, a comparative phenotype analysis of primary human MC in culture using 191 monoclonal antibodies directed against 108 antigens was performed by flow-cytometry. The MC were grown on three different surfaces (human matrix, fibronectin, polystyrene) and cultured in the presence or absence of IL-1alpha. Seventy-one antibodies recognizing 35 different antigens (integrins: CD29, 49b, 49c, 49e, 51, 61; immunoglobulin gene family: CD54, 58, 90, 106, 146, 147, 166; growth factor receptors: CD105, 140b; apoptosis related: CD95; hemostatis related: CD141, 142; miscellaneous: CD44, 109, 138, 151, 157, 165, and 11 nonclustered antigens) reacted with mesangial cells. CD58, 109, 146, 147, 151, 157, 165, and 166 are reported for the first time to be present on human mesangial cells. In comparison to growth on polystyrene, CD44, 54, 95, 105, 109, 140b, 146, 147, 157, 165 and 166, were up-regulated on fibronectin, and CD44, 54, 90, 95, 105, 106, 109, 138, 140b, 141, 142, 146, 147, 151, 157, 165 and 166 were up-regulated on human matrix. The stimulation by IL-1alpha up-regulated CD44, 49e, 51, 54, 61, 106 on MC on polystyrene; CD49e, 51, 61, 106, 146, 165 on MC on fibronectin, and CD49e, 51, 54 on MC grown on human matrix. This analysis of surface antigen expression provides new information to enable a better understanding of the role of mesangial cells in glomerular pathophysiology.

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“…Taking into consideration that both mesangial cells and podocytes contain vimentin, the 2-3-day-old outgrowths from glomerular explants were simultaneously labelled with GFAP and desmin, the marker of mesangial cells (Barnes et al, 1995;Holthofer et al, 1995;Cluzeaud et al, 1996;Burg et al, 1997), which is absent from cobblestone-like podo-cytes (Mundel et al, 1997). Taking into consideration that both mesangial cells and podocytes contain vimentin, the 2-3-day-old outgrowths from glomerular explants were simultaneously labelled with GFAP and desmin, the marker of mesangial cells (Barnes et al, 1995;Holthofer et al, 1995;Cluzeaud et al, 1996;Burg et al, 1997), which is absent from cobblestone-like podo-cytes (Mundel et al, 1997).…”

Section: Recently Investigationsmentioning

confidence: 99%

“…Therefore, further experiments were performed on cultures of glomerular explants derived from lo-day-old rat kidneys. Taking into consideration that both mesangial cells and podocytes contain vimentin, the 2-3-day-old outgrowths from glomerular explants were simultaneously labelled with GFAP and desmin, the marker of mesangial cells (Barnes et al, 1995;Holthofer et al, 1995;Cluzeaud et al, 1996;Burg et al, 1997), which is absent from cobblestone-like podo-cytes (Mundel et al, 1997). In these early cultures two types of cells were observed: process-lacking and process-bearing cells.…”

Section: Recently Investigationsmentioning

confidence: 99%

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The immunoreactivity of glial fibrillary acidic protein in mesangial cells and podocytes of the glomeruli of rat kidney in vivo and in culture

Buniatian

Traub

Albinus³

et al. 1998

Biology of the Cell

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In this study the presence of glial fibrillary acidic protein (GFAP) in kidney is for the first time demonstrated in cryostat sections and cultures of isolated glomerular explants derived from rat kidneys. In double immunolabelling analysis of adult rat kidney sections using antiserum against GFAP and monoclonal antibody (mAb) against vimentin or desmin, the presence of immunoreactivity for GFAP could be observed in the glomerulus of the kidney and vascular cells situated in the peritubular space which expressed vimentin and desmin. Labelling of the sections with absorbed antiserum against GFAP completely abolished the staining in all these cells. The mAb against GFAP, clone GF12.24 which is known to label GFAP both in neural and non-neural cells, recognised its antigen only in the cells located in glomeruli. The investigations performed on early 2- or 3-day-old cultures from glomerular explants revealed different patterns of staining for GFAP in mesangial cells and podocytes: weak filamentous in mesangial cells and a strong non-filamentous perinuclear pattern in podocytes. Due to prominent perinuclear expression in podocytes GFAP may be considered as a marker of these cells. A different pattern of distribution of immunoreactivity for GFAP in podocytes and mesangial cells might be due to function-related posttranslational modifications of GFAP resulting in assembly or disassembly of GFAP filaments. The different pattern of staining for GFAP in the podocytes and mesangial cells, cells which exert a different influence on the capillaries of the glomeruli, suggests a role for GFAP in regulation of the tension and permeability of vascular walls. Previous investigations and present studies hint at GFAP as being a general marker of perivascular cells.

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Glomerular antigens in severe hereditary nephrosis

Cited by 4 publications

References 15 publications

Glial fibrillary acidic protein‐positive cells of the kidney are capable of raising a protective biochemical barrier similar to astrocytes: Expression of metallothionein in podocytes

Glial fibrillary acidic protein‐positive cells of the kidney are capable of raising a protective biochemical barrier similar to astrocytes: Expression of metallothionein in podocytes

Surface antigens of human mesangial cells: impact of growth surface or IL‐1α

The immunoreactivity of glial fibrillary acidic protein in mesangial cells and podocytes of the glomeruli of rat kidney in vivo and in culture

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