Glomerular Loss After Renal Radiofrequency Ablation Are Comparable to 30 Minutes of Warm Ischemia

Abreu, L.; Damasceno-Ferreira, José Aurelino; Costa, Waldemar S.; Pereira‐Sampaio, Marco A.; Sampaio, Francisco J.B.; Souza, Diogo Benchimol de

doi:10.1089/end.2016.0899

Cited by 6 publications

(12 citation statements)

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“…Kidneys were weighed and their volumes were estimated by the Scherle’s method [10]. The cortical-medullar ratio was estimated using Cavalieri’s principle [11, 12], and the absolute cortical volume (CV) was calculated by multiplying the cortical-medullary ratio by the renal volume.…”

Section: Methodsmentioning

confidence: 99%

Does a Ureteral Obstruction Affect the Contralateral Kidney Morphology? A Stereological Analysis in a Rodent Model

et al. 2018

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Objective: To investigate, in a rodent model, the influence of an obstructed kidney/ureter on the contralateral organ morphology. Methods: Thirty-six rats were randomly assigned into the following groups: untreated ureteral obstruction group (UO-U, n = 10), submitted to a ligature of the ureter at day 0; UO group (at day 0) followed by nephrectomy after 24 h (UO-N, n = 8); nephrectomy group (N, n = 9), submitted to nephrectomy at day 0; and sham group (S, n = 9), submitted to simulated surgery at day 0. All these procedures were performed on the left kidney/ureter. All animals were euthanized 30 days after surgery, and the right kidneys were collected for stereological analysis. Data were compared using a one-way analysis of variance with Bonferroni’s post test, considering p < 0.05 as significant. Results: The kidney volume, weight, and cortical volume were augmented in groups UO-N and N, compared to group S. Kidneys from the UO-U group showed only a moderate augmentation in kidney weight, with a reduction in the cortical-medullar ratio, compared to the kidneys in group S. No differences in the glomerular volumetric density, volume-weighted glomerular volume, and number of glomeruli were observed among the right kidneys of the studied groups. Conclusion: An obstructed kidney/ureter is more prejudicial to the contralateral kidney morphology than an absent kidney.

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Section: Methodsmentioning

confidence: 99%

Does a Ureteral Obstruction Affect the Contralateral Kidney Morphology? A Stereological Analysis in a Rodent Model

et al. 2018

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“…The kidneys were harvested, weighed, and their volumes measured by the Scherle's method 17,18 ; then, the organs were fixed by immersion in 4% buffered formaldehyde according to our laboratory routine. Samples were collected from the cortical region of these 64 kidneys and were processed by routine histological methods for stereological analysis.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Samples were collected from the cortical region of these 64 kidneys and were processed by routine histological methods for stereological analysis. Each kidney was sectioned longitudinally and from the created cut surface, samples were collected randomly, in the same fashion as in previous studies 14,18 . The specimens were paraffin embedded, sectioned at 5-µm thickness, and stained by hematoxylin & eosin.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The specimens were paraffin embedded, sectioned at 5-µm thickness, and stained by hematoxylin & eosin. The cortical-medullar ratio was estimated by using the point-counting-method according the Cavalieri principle 18,19 . The absolute cortical volume (CV) was calculated by multiplying the cortical-medullar ratio by the renal volume.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The volume weighted mean glomerular volume (VWGV), was estimated by using the point-sampled intercepts method 14,16,18,22,23 , analyzing 50 glomeruli per kidney.…”

Section: ■ Introductionmentioning

confidence: 99%

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The relationship between renal warm ischemia time and glomerular loss. An experimental study in a pig model

et al. 2017

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The relationship between renal warm ischemia time and glomerular loss. An experimental study in a pig model 1 Acta Cir. Bras. 2017;32(5):334-341 Abstract Purpose: To investigate the glomerular number after different warm ischemia times. Methods: Thirty two pigs were assigned into four groups. Three groups (G10, G20, and G30) were treated with 10, 20, and 30 minutes of left renal warm ischemia. The sham group underwent the same surgery without renal ischemia. The animals were euthanized after 3 weeks, and the kidneys were collected. Right kidneys were used as controls. The kidney weight, volume, cortical-medullar ratio, glomerular volumetric density, volume-weighted mean glomerular volume, and the total number of glomeruli per kidney were obtained. Serum creatinine levels were assessed pre and postoperatively. Results: Serum creatinine levels did not differ among the groups. All parameters were similar for the sham, G10, and G20 groups upon comparison of the right and left organs. The G30 group pigs' left kidneys had lower weight, volume, and cortical-medullar ratio and 24.6% less glomeruli compared to the right kidney. A negative correlation was found between warm ischemia time and glomerular number. Conclusions: About one quarter of glomeruli was lost after 30 minutes of renal warm ischemia. No glomeruli loss was detected before 20 minutes of warm ischemia. However, progressive glomerular loss was associated with increasing warm ischemia time.

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Volume and Shape Assessment of Renal Radiofrequency Ablation Lesion

Abreu

Damasceno-Ferreira

Monteiro

et al. 2018

Urology

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Glomerular Loss After Renal Radiofrequency Ablation Are Comparable to 30 Minutes of Warm Ischemia

Abstract: RFA in pigs determines a significant reduction of glomerular density in the remaining parenchyma. This alteration was comparable to that observed in kidneys submitted to 30 minutes of warm ischemia.

Cited by 6 publications

References 29 publications

Does a Ureteral Obstruction Affect the Contralateral Kidney Morphology? A Stereological Analysis in a Rodent Model

Does a Ureteral Obstruction Affect the Contralateral Kidney Morphology? A Stereological Analysis in a Rodent Model

The relationship between renal warm ischemia time and glomerular loss. An experimental study in a pig model

Volume and Shape Assessment of Renal Radiofrequency Ablation Lesion

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