GloPID-R report on Chikungunya, O'nyong-nyong and Mayaro virus, part I: Biological diagnostics

Pezzi, Laura; Reusken, Chantal; Weaver, Scott C.; Drexler, Jan Felix; Busch, Michael P.; LaBeaud, A. Desirée; Diamond, Michael S.; Vasilakis, Nikos; Drebot, Michael A.; Siqueira, André Machado; Ribeiro, Guilherme S.; Kohl, Alain; Lecuit, Marc; Ng, Lisa F. P.; Gallian, Pierre; Lamballerie, Xavier de; Boyer, Sébastien; Brasil, Patrícia; Diallo, Mawlouth; Failloux, Anna‐Bella; Jaenisch, Thomas; Lourenço‐de‐Oliveira, Ricardo; Neyts, Johan; Rios, María; Rodriguez-Morales, A.J.; Rosa-Freitas, María Goreti; Sall, Amadou Alpha; Simmons, Graham; Simon, Fabrice; Rúa, Anubis Vega

doi:10.1016/j.antiviral.2019.03.009

Cited by 35 publications

(54 citation statements)

References 107 publications

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“…Moreover, in such regions of relatively higher immunity, local residents are hence less likely to present with symptomatic infections [70,71]. We have previously noted that the presence of CHIKV in coastal Kenya may be misinterpreted by the presence of a closely related alphavirus, O'nyong'nyong virus [65], which is transmitted by anopheline mosquitoes [65], yet indistinguishable from CHIKV when utilizing common serological diagnostic tools due to antibody cross-reactivity [72]. Yet, repeated discovery of arboviruses in competent vectors and in human populations reiterates the public health importance of these findings, and the need for better integration into local vector control measures and community education.…”

Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%

Evidence of transovarial transmission of Chikungunya and Dengue viruses in field-caught mosquitoes in Kenya

et al. 2020

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Arboviruses are among the most important emerging pathogens due to their increasing public health impact. In Kenya, continued population growth and associated urbanization are conducive to vector spread in both urban and rural environments, yet mechanisms of viral amplification in vector populations is often overlooked when assessing risks for outbreaks. Thus, the characterization of local arbovirus circulation in mosquito populations is imperative to better inform risk assessments and vector control practices. Aedes species mosquitoes were captured at varying stages of their life cycle during different seasons between January 2014 and May 2016 at four distinct sites in Kenya, and tested for chikungunya (CHIKV), dengue (DENV) and Zika (ZIKV) viruses by RT-PCR. CHIKV was detected in 45 (5.9%) and DENV in 3 (0.4%) mosquito pools. No ZIKV was detected. Significant regional variation in prevalence was observed, with greater frequency of CHIKV on the coast. DENV was detected exclusively on the coast. Both viruses were detected in immature mosquitoes of both sexes, providing evidence of transovarial transmission of these arboviruses in local mosquitoes. This phenomenon may be driving underlying viral maintenance that may largely contribute to periodic re-emergence among humans in Kenya.

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Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%

Evidence of transovarial transmission of Chikungunya and Dengue viruses in field-caught mosquitoes in Kenya

et al. 2020

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“…Most mosquito-borne viral infections are asymptomatic or non-specific mild infections (Endy et al, 2011). Symptomatic infections are often identified as a systemic febrile illness with non-specific symptoms, thus making mosquito-borne diseases (MBDs) very difficult to diagnose (Pezzi et al, 2019). Headache, weakness and rashes are common manifestations.…”

Section: Clinical Aspectsmentioning

confidence: 99%

The Role of Temperature in Shaping Mosquito-Borne Viruses Transmission

Bellone

Failloux

2020

Front. Microbiol.

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Mosquito-borne diseases having the greatest impact on human health are typically prevalent in the tropical belt of the world. However, these diseases are conquering temperate regions, raising the question of the role of temperature on their dynamics and expansion. Temperature is one of the most significant abiotic factors affecting, in many ways, insect vectors and the pathogens they transmit. Here, we debate the veracity of this claim by synthesizing current knowledge on the effects of temperature on arboviruses and their vectors, as well as the outcome of their interactions.

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“…1B) (5). As alphavirus viremia is short-lived, serologic detection of virus-specific antibodies is required for patient diagnostics and sero-epidemiologic studies (6,7). Diagnostics in public health laboratories demand robust high-throughput tests, such as enzyme-linked immunosorbent assays (ELISAs) (7).…”

mentioning

confidence: 99%

“…As alphavirus viremia is short-lived, serologic detection of virus-specific antibodies is required for patient diagnostics and sero-epidemiologic studies (6,7). Diagnostics in public health laboratories demand robust high-throughput tests, such as enzyme-linked immunosorbent assays (ELISAs) (7). To systematically assess serologic testing of MAYV and CHIKV, we assembled a panel comprising 37 MAYV-specific sera from Peru and 64 CHIKV-specific sera from Brazil (8), including longitudinally collected samples (6) ( Table 1).…”

mentioning

confidence: 99%

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Robustness of Serologic Investigations for Chikungunya and Mayaro Viruses following Coemergence

Fischer

Bozza

Merino

et al. 2020

mSphere

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Since 2013, the arthropod-borne Chikungunya virus (CHIKV) has cocirculated with the autochthonous Mayaro virus (MAYV) in Latin America. Both belong to the same alphavirus serocomplex, termed the Semliki Forest serocomplex. The extent of antibody cross-reactivity due to the antigenic relatedness of CHIKV and MAYV in commonly used serologic tests remains unclear. By testing 64 CHIKV- and 37 MAYV-specific sera from cohort studies conducted in Peru and Brazil, we demonstrate about 50% false-positive test results using commercially available enzyme-linked immunosorbent assays (ELISAs) based on structural antigens. In contrast, combining ELISAs for CHIKV and MAYV significantly increased positive predictive values (PPV) among all cohorts from 35.3% to 88.2% for IgM and from 61.3% to 96.8% for IgG (P < 0.0001). Testing of longitudinally collected CHIKV-specific patient sera indicated that ELISA specificity is highest for IgM testing at 5 to 9 days post-onset of symptoms (dpo) and for IgG testing at 10 to 14 dpo. IgG cross-reactivity in ELISA was asymmetric, occurring in 57.9% of MAYV-specific sera compared to 29.5% of CHIKV-specific sera. Parallel plaque reduction neutralization testing (PRNT) for CHIKV and MAYV increased the PPV from 80.0% to 100% (P = 0.0053). However, labor-intense procedures and delayed seroconversion limit PRNT for patient diagnostics. In sum, individual testing for CHIKV or MAYV only is prone to misclassifications that dramatically impact patient diagnostics and sero-epidemiologic investigation. Parallel ELISAs for both CHIKV and MAYV provide an easy and efficient solution to differentiate CHIKV from MAYV infections. This approach may provide a template globally for settings in which alphavirus coemergence imposes similar problems. IMPORTANCE Geographically overlapping transmission of Chikungunya virus (CHIKV) and Mayaro virus (MAYV) in Latin America challenges serologic diagnostics and epidemiologic surveillance, as antibodies against the antigenically related viruses can be cross-reactive, potentially causing false-positive test results. We examined whether widely used ELISAs and plaque reduction neutralization testing allow specific antibody detection in the scenario of CHIKV and MAYV coemergence. For this purpose, we used 37 patient-derived MAYV-specific sera from Peru and 64 patient-derived CHIKV-specific sera from Brazil, including longitudinally collected samples. Extensive testing of those samples revealed strong antibody cross-reactivity in ELISAs, particularly for IgM, which is commonly used for patient diagnostics. Cross-neutralization was also observed, albeit at lower frequencies. Parallel testing for both viruses and comparison of ELISA reactivities and neutralizing antibody titers significantly increased diagnostic specificity. Our data provide a convenient and practicable solution to ensure robust differentiation of CHIKV- and MAYV-specific antibodies.

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GloPID-R report on Chikungunya, O'nyong-nyong and Mayaro virus, part I: Biological diagnostics

Cited by 35 publications

References 107 publications

Evidence of transovarial transmission of Chikungunya and Dengue viruses in field-caught mosquitoes in Kenya

Evidence of transovarial transmission of Chikungunya and Dengue viruses in field-caught mosquitoes in Kenya

The Role of Temperature in Shaping Mosquito-Borne Viruses Transmission

Robustness of Serologic Investigations for Chikungunya and Mayaro Viruses following Coemergence

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