Glossina fuscipes fuscipes and Glossina palpalis palpalis as joint vectors of sleeping sickness in the focus of Nola-Bilolo in the Central African Republic

Gouteux, Jean‐Paul; D’Amico, Frank; Gboumbi, J.C. Kounda; Noutoua, L.; Bailly, Clément

doi:10.1016/0001-706x(92)90059-7

Cited by 3 publications

(1 citation statement)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The data presented here are, however, preliminary and the observations and apparent associations made need to be confirmed in larger investigations, including entomological (Laveissière and Grébaut, 2003) and matched case-control studies. Similar observations have been made in the Central African Republic (Gouteux et al, 1993). The fact that many HAT cases in and around Bengo are not infected in their places of residence clearly means that T. b. gambiense is being dispersed as the cases travel and work away from their homes and return to their homes.…”

Section: Hat Case Profilessupporting

confidence: 74%

Epidemiological aspects of the transmission of the parasites causing human African trypanosomiasis in Angola

Truc

Grébaut

Lando

et al. 2011

Annals of Tropical Medicine & Parasitology

View full text Add to dashboard Cite

Section: Hat Case Profilessupporting

confidence: 74%

Epidemiological aspects of the transmission of the parasites causing human African trypanosomiasis in Angola

Truc

Grébaut

Lando

et al. 2011

Annals of Tropical Medicine & Parasitology

View full text Add to dashboard Cite

Tsetse flies, biodiversity and the control of sleeping sickness. Structure of a Glossina guild in southwest Côte d'Ivoire

Gouteux

Jarry

1998

Acta Oecologica

View full text Add to dashboard Cite

Genetic diversity and phylogenetic relationships of tsetse flies of the palpalis group in Congo Brazzaville based on mitochondrial cox1 gene sequences

et al. 2020

View full text Add to dashboard Cite

Background Despite the morphological characterization established in the 1950s and 1960s, the identity of extant taxa that make up Glossina fuscipes (s.l.) in the Congo remains questionable. Previous claims of overlap between G. fuscipes (believed to be G. f. quanzensis) and G. palpalis palpalis around Brazzaville city further complicate the taxonomic status and population dynamics of the two taxa. This study aimed to determine the phylogenetic relationships between G. fuscipes (s.l.) and G. p. palpalis and to assess genetic variation among G. fuscipes (s.l.) populations in Congo Brazzaville. Methods We collected 263 G. fuscipes (s.l.) from northern and central regions, and 65 G. p. palpalis from southern part of the country. The mitochondrial cytochrome c oxidase subunit 1 (cox1) gene was amplified using taxa-specific primer pairs. Sequence data were analyzed in DnaSP and Arlequin to assess the genetic diversity, differentiation and demographic history of G. fuscipes (s.l.) populations. Results The general BLAST analysis yielded a similarity of 99% for G. fuscipes (s.l.) and G. p. palpalis. BLASTn analysis for G. fuscipes (s.l.) showed > 98% identity with GenBank sequences for G. fuscipes (s.l.), with BEMB population showing 100% similarity with G. f. fuscipes. Glossina fuscipes (s.l.) populations showed high haplotype diversity (H = 46, Hd = 0.884), moderate nucleotide diversity ( = 0.012) and moderate (FST = 0.072) to high (FST = 0.152) genetic differentiation. Most of the genetic variation (89.73%) was maintained within populations. The mismatch analysis and neutrality tests indicated recent tsetse population expansions. Conclusions Phylogenetic analysis revealed minor differences between G. fuscipes (s.l.) and G. p. palpalis. Genetic diversity of G. fuscipes (s.l.) was high in the populations sampled except one. Genetic differentiation ranged from moderate to high among subpopulations. There was a restricted gene flow between G. fuscipes (s.l.) populations in the north and central part of the country. Genetic signatures based on cox1 showed recent expansion and recovery of G. fuscipes (s.l.) populations from previous bottlenecks. To fully understand the species distribution limits, we recommend further studies involving a wider sampling scheme including the swampy Mossaka focus for G. fuscipes (s.l.) and the entire range of G. p. palpalis in South Congo.

show abstract

Glossina fuscipes fuscipes and Glossina palpalis palpalis as joint vectors of sleeping sickness in the focus of Nola-Bilolo in the Central African Republic

Cited by 3 publications

References 1 publication

Epidemiological aspects of the transmission of the parasites causing human African trypanosomiasis in Angola

Epidemiological aspects of the transmission of the parasites causing human African trypanosomiasis in Angola

Tsetse flies, biodiversity and the control of sleeping sickness. Structure of a Glossina guild in southwest Côte d'Ivoire

Genetic diversity and phylogenetic relationships of tsetse flies of the palpalis group in Congo Brazzaville based on mitochondrial cox1 gene sequences

Contact Info

Product

Resources

About