Glow discharge modified tissue culture polystyrene: Role of surface chemistry in cellular attachment and proliferation

PMMA/PS copolymers were synthesized by radical polymerization techniques. And the 1H NMR results illustrate that the molar contents of PMMA are respectively 19%, 53%, 69% and 84%. The coatings of those PMMA/PS copolymers were prepared by spin-coating. The surface structures and protein resistance of copolymer coatings were investigated respectively by AFM and BSA absorption experiment. It is showed that the copolymer coating surfaces have micro-phase separation structure. Furthermore, with the increase in the PMMA content, the surface pattern changes from interpenetration network pattern to island structure that the PMMA-rich phase surrounded by the PS-rich phase. In addition, all coatings prepared in this study, especially the coating with PMMA content of 69%, have good resistance to BSA protein.

show abstract

“…The signals at 6.4-7.2 ppm (Spec. b) could be assigned to the protons of benzene rings in polystyrene [12,13]. Moreover the peak area of Spec.…”

Section: Resultsmentioning

confidence: 98%

Surface Structure and Protein Resistance of PMMA/PS Copolymer Coatings

Zhang

Guo

et al. 2012

AMM

View full text Add to dashboard Cite

show abstract

“…Another explanation could be the presence of C = O functional groups in the plasma polymer. It has been shown that the surface concentration of carbonyl groups influences the cellular adhesion of Chinese ovary hamster [23] and bovine aortic [24] cell lines. As this functional group was only observed on the Ar/C 2 H 2 plasmatreated sample (Fig.…”

Section: Cells and Plasma Polymer Interactionsmentioning

confidence: 99%

Atmospheric Pressure Plasma Jet Deposition of Patterned Polymer Films for Cell Culture Applications

Leduc

Coulombe

Leask

2009

IEEE Trans. Plasma Sci.

View full text Add to dashboard Cite

A miniature nonthermal atmospheric plasma jet was used to deposit patterns of plasma polymers on standard Pyrex Petri dishes from an argon/acetylene gas mixture. The injection of the C 2 H 2 monomer through the powered capillary electrode ending in the nozzle section allowed a stable operation of the plasma jet and the successful deposition of plasma-polymer tracks ≈500 μm wide. The line-averaged gas temperature was measured from the OH molecular band emission originating from the entrainment of air into the plasma jet. The gas temperature decreased monotonically from ≈440 K at the nozzle exit to ≈385 K at 5 mm downstream. The emission profiles of the C 2 (516 nm) band showed a peak ≈0.5 mm downstream the nozzle exit. The maximum emission for the CH band (431 nm) was obtained at the torch exit. An ATR-FTIR spectroscopy analysis of the deposited plasma-polymer films revealed the presence of C-H, O-H, and C = O functional groups. Mammalian cells grown on the argon/acetylene plasma-treated dishes showed migration and greater density compared to the control and dishes treated with an argon plasma jet. This preliminary study opens the door to the localized enhancement of cell coverage on various substrates (shape and material).Index Terms-Atmospheric pressure glow discharge (APGD) jet, cell growth and proliferation, plasma polymer, plasma spectroscopy.

show abstract

Pulsed‐Plasma‐Induced Micropatterning with Alternating Hydrophilic and Hydrophobic Surface Chemistries

Malkov

Martin

Schwisow

et al. 2008

Plasma Processes & Polymers

View full text Add to dashboard Cite

High‐fidelity micrometer‐scale patterns of alternating hydrophobic and hydrophilic materials were created on polystyrene using pulsed plasma polymerization. The hydrophobic material was formed using C3F8. Grids with 80–100 µm holes were used to generate patterns with acrylic acid, 2‐hydroxyethyl methacrylate, N‐vinyl‐2‐pyrrolidinone, N‐vinylformamide, allylamine, and hexylamine. The materials were characterized with angle‐resolved XPS, spectroscopic ellipsometry, and static CA measurements. Excellent pattern fidelity with all monomers was confirmed with SEM, SAM, XPS imaging, and static ToF‐SIMS imaging.

show abstract

Glow discharge modified tissue culture polystyrene: Role of surface chemistry in cellular attachment and proliferation

Cited by 4 publications

References 43 publications

Surface Structure and Protein Resistance of PMMA/PS Copolymer Coatings

Surface Structure and Protein Resistance of PMMA/PS Copolymer Coatings

Atmospheric Pressure Plasma Jet Deposition of Patterned Polymer Films for Cell Culture Applications

Pulsed‐Plasma‐Induced Micropatterning with Alternating Hydrophilic and Hydrophobic Surface Chemistries

Contact Info

Product

Resources

About