Glucagon-like peptide-1 and beta cell glucose sensitivity - a glucose ramp study in mice

Åhrén, Bo

doi:10.1016/j.peptides.2021.170650

Cited by 1 publication

(3 citation statements)

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“…The second study was a hyperglycemic clamp, where glucose levels were gradually increased during 2 min, whereafter they stayed constant [ 26 ]. The third study was a hyperglycemic ramp when glucose was slowly increased over 40 min [ 27 ]. The absolute increase in glucose levels was approximately the same in these three studies, but there was a difference in time pattern.…”

Section: Discussionmentioning

confidence: 99%

“…The absolute increase in glucose levels was approximately the same in these three studies, but there was a difference in time pattern. The resulting increase in insulin levels was lower with a slower increase in glucose levels, particularly notable during the glucose ramp when beta cells responded very slowly and no clear first phase but only a gradual second phase was evident [ 27 ]. Nevertheless, under these conditions, it was shown that there was a marked augmentation of glucose-stimulated insulin secretion by GLP-1 [ 27 ], which showed that under in vivo conditions, glucose was only one regulator of beta cell function.…”

Section: Discussionmentioning

confidence: 99%

“… Glucose levels ( A ) and increase in insulin levels (delta insulin) ( B ) during hyperglycaemic clamp (data from [ 26 ]), glucose ramp (data from [ 27 ]), and intravenous glucose (at 0.35 g/kg) (data from present study) in C57BL/6J mice. Means ± SEM are shown.…”

Section: Figurementioning

confidence: 99%

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The Glucose Sensitivity of Insulin Secretion-Lessons from In Vivo and In Vitro Studies in Mice

Åhrén

2022

Biomolecules

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This study explored the relationship between the glucose dose and insulin response from beta cells in vivo and in vitro in mice. Glucose was administered intravenously at different dose levels (from 0 to 0.75 g/kg) in anesthetized C57BL/6J mice, and the glucose and insulin concentrations were determined in samples taken after 50 min. Furthermore, freshly isolated mouse islets were incubated for 60 min in the presence of different concentrations of glucose (from 2.8 to 22.2 mmol/L) and insulin levels were analyzed in the medium. It was found that insulin levels increased after an intravenous injection of glucose with the maximal increase seen after 0.35 g/kg with no further increase after 0.5 or 0.75 g/kg. The acute increase in insulin levels (during the first 5 min) and the maximum glucose level (achieved after 1 min) showed a curvilinear relation with the half-maximal increase in insulin levels achieved at 11.4 mmol/L glucose and the maximal increase in insulin levels at 22.0 mmol/L glucose. In vitro, there was also a curvilinear relation between glucose concentrations and insulin secretion. Half maximal increase in insulin concentrations was achieved at 12.5 mmol/L glucose and the maximal increase in insulin concentrations was achieved at 21.5 mmol/L. Based on these data, we concluded that the glucose-insulin relation was curvilinear both in vivo and in vitro in mice with similar characteristics in relation to which glucose levels that achieve half-maximal and maximal increases in insulin secretion. Besides the new knowledge of knowing these relations, the results have consequences on how to design studies on insulin secretion to obtain the most information.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%