Glucan stimulates human dermal fibroblast collagen biosynthesis through a nuclear factor‐1 dependent mechanism

Abstract

Glucan, an immunomodulator, has been reported to increase collagen deposition and tensile strength in experimental models of wound repair. Previous data suggest that glucan modulates wound healing via an indirect mechanism in which macrophages are stimulated to release growth factors and cytokines. However, recent data have shown the presence of glucan receptors on normal human dermal fibroblasts, suggesting that glucans may be able to directly stimulate fibroblast collagen biosynthesis. To test this hypothesis, we examined the effect of glucan on collagen biosynthesis in normal human dermal fibroblasts. We assessed nuclear factor‐1 (NF‐1) activation, procollagen mRNA expression, collagen biosynthesis, and whether there was a causal link between glucan treatment, NF‐1 activation, and collagen expression. Glucan (1 µg/ml) increased NF‐1 binding activity by 46% (8 hours), 64% (24 hours), 215% (36 hours), and 119% (48 hours) in cultured normal human dermal fibroblasts. Alpha 1(I) and α1 (III) procollagen mRNA were increased in glucan‐treated normal human dermal fibroblasts when compared with the untreated fibroblasts. Collagen synthesis was increased at 24 hours and 48 hours following glucan treatment of normal human dermal fibroblasts. Down‐regulation of NF‐1 by pentifylline inhibited glucan‐induced procollagen mRNA expression. These data indicate that glucan can directly stimulate human fibroblast collagen biosynthesis through an NF‐1–dependent mechanism. ( WOUND REP REG 2002;10:168)

Chemical composition of the cell wall of probiotic and brewer’s yeast in response to cultivation medium with glycerol as a carbon source

“…Polymers of yeasts cell wall exhibit an ability to adsorb harmful chemical substances, for example, mycotoxins, they lower the level of cholesterol and triglycerides in blood, act in anti-mutagenic, anticancerous, antioxidative way, and protect against UV radiations [17,29,41,45,54]. According to other authors [55], b-glucan of yeast origin stimulates collagen biosynthesis which is a significant component in wound-healing process.…”

“…They recognize b-glucans and mannoprotein as pathogen-associated molecular patterns (PAMPs) [31,45]. Significance of polysaccharides of cell wall in yeasts in stimulation of immunological response, antibacterial activity as well as probiotic one with respect to beneficial microflora of alimentary tract is emphasized [6,9,13,26,37,40,47,51,55]. Polymers of yeasts cell wall exhibit an ability to adsorb harmful chemical substances, for example, mycotoxins, they lower the level of cholesterol and triglycerides in blood, act in anti-mutagenic, anticancerous, antioxidative way, and protect against UV radiations [17,29,41,45,54].…”

Effect of beta-glucan in lung damage secondary to experimental obstructive jaundice

“…Owing to their potent stimulatory effect on macrophages, they increase the cytotoxicity and phagocytic capacity of macrophages (12). Literature reports indicate that beta-glucans exhibit antioxidant, antiviral, antibacterial, and antifungal activities, and have an ameliorative effect on wounds (13)(14)(15)(16)(17)(18)(19).…”

Chitotirosidase and prolidase in tinea pedis: A preliminary study

“…The enzyme activity plays an important role in the recycling of proline mostly derived from degradation products of collagen [29]. Wei et al [30] have been reported that glucan stimulates human dermal fibroblast collagen biosynthesis through a nuclear factor-1 dependent mechanism. In our study, we did not find any difference between patients and controls in terms of prolidase activity.…”