Glucanolytic Actinomycetes Antagonistic to Phytophthora fragariae var. rubi, the Causal Agent of Raspberry Root Rot

Valois, Diane; Fayad, K P; Barasubiye, Tharcisse; Garon, M; Déry, Claude V.; Brzeziński, Ryszard; Beaulieu, Carole

doi:10.1128/aem.62.5.1630-1635.1996

Cited by 137 publications

(49 citation statements)

References 39 publications

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“…Phytophthora fragariae (Valois et al 1996) and P. aphanidermatum (El-Tarabily 2006). Pythium hyphae have been reported to contain mainly the noncellulosic b-1,3-and b-1,6-linked glucans as the predominant glucans in addition to the cellulosic b-1,4-linked glucan, which are present as a relatively small proportion of the total glucans (Sietsma et al 1975).…”

Section: Discussionmentioning

confidence: 99%

“…(El-Tarabily 2006) and Phytophthora spp. (Valois et al 1996). It is also interesting that production of siderophores was not involved as a mechanism of biocontrol, although siderophore-mediated competition for iron has been reported to be an important mechanism of biological control of Pythium diseases by bacterial antagonists (Matthijs et al 2007).…”

Section: Discussionmentioning

confidence: 99%

“…All endophytic isolates were initially screened for their ability to produce clear zones on P. aphanidermatum mycelial fragment agar (MFA) as described by Valois et al (1996). Clear zone diameters were measured (mm) and used as a preliminary indicator of glucanolytic activity.…”

Section: In Vitro Antagonistic Activities Against Pythium Aphanidermatummentioning

confidence: 99%

“…The isolates that totally inhibited P. aphanidermatum growth were selected for subsequent tests detailed below. Quantitative determination of b-1,3, b-1,4 and b-1,6-glucanases was made as described by Valois et al (1996) using minimal salt broth medium (MSBM) amended with 2AE5 mg ml )1 of either laminarin (Sigma), cellulose (Sigma) or pustulan (Calbiochem, San Diego, CA, USA), respectively. The effects of the antagonistic isolates on living hyphae were determined on MSAM, amended with living mycelium of P. aphanidermatum to serve as the sole carbon source as described by Sneh (1981).…”

Section: In Vitro Antagonistic Activities Against Pythium Aphanidermatummentioning

confidence: 99%

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Plant growth promotion and biological control ofPythium aphanidermatum, a pathogen of cucumber, by endophytic actinomycetes

El‐Tarabily

Nassar

Hardy

et al. 2009

Journal of Applied Microbiology

235

108

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Aims: To evaluate the potential of Actinoplanes campanulatus, Micromonospora chalcea and Streptomyces spiralis endophytic in cucumber roots, to promote plant growth and to protect seedlings and mature plants of cucumber from diseases caused by Pythium aphanidermatum, under greenhouse conditions. Methods and Results: Three endophytic isolates, out of 29, were selected through tests aimed at understanding their mechanisms of action as biocontrol agents and plant growth promoters. When applied individually or in combination, they significantly promoted plant growth and reduced damping‐off and crown and root rot of cucumber. The combination of the three isolates resulted in significantly better suppression of diseases and plant growth promotion, than where the plants were exposed to individual strains. Conclusions: The three selected actinomycete isolates colonized cucumber roots endophytically for 8 weeks, promoted plant growth and suppressed pathogenic activities of P. aphanidermatum on seedling and mature cucumber plants. Significance and Impact of the Study: The results clearly show that the endophytic, glucanase‐producing actinomycetes used, especially as a combined treatment, could replace metalaxyl, which is the currently recommended fungicide for Pythium diseases in the United Arab Emirates. These endophytic isolates also have the potential to perform as plant growth promoters, which is a useful attribute for crop production in nutrient impoverished soils.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: In Vitro Antagonistic Activities Against Pythium Aphanidermatummentioning

confidence: 99%

Section: In Vitro Antagonistic Activities Against Pythium Aphanidermatummentioning

confidence: 99%

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Plant growth promotion and biological control ofPythium aphanidermatum, a pathogen of cucumber, by endophytic actinomycetes

El‐Tarabily

Nassar

Hardy

et al. 2009

Journal of Applied Microbiology

235

108

View full text Add to dashboard Cite

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“…are generally saprophytic, soil-dwelling microorganisms that spend the majority of their life cycle as spores. It has also been demonstrated that actinomycetes are important microorganisms in the rhizosphere and their antagonism to phytopathogenic fungi has been demonstrated [1]. Some actinobacteria are also known to inhabit the tissues of healthy plants.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of endophytic streptomycete antagonists of fusarium wilt pathogen from surface-sterilized banana roots

Cao¹,

Qiu²,

You³

et al. 2005

FEMS Microbiology Letters

238

121

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A total of 131 endophytic actinomycete strains were successfully isolated from surface-sterilized banana roots. These isolates belonged to Streptomyces (n=99), Streptoverticillium (n=28), and Streptosporangium (n=2) spp. The remaining 2 isolates were not identified. About 18.3% of the isolates inhibited the growth of pathogenic Fusarium oxysporum f. sp. cubense on banana tissue extract medium. The most frequently isolated Streptomyces sp. strain S96 was similar to Streptomyces griseorubiginosus. About 37.5% of the S. griseorubiginosus strains were antagonistic to F. oxysporum f. sp. cubense. The antagonism of strain S96 was lost when FeCl(3) was introduced into the inhibition zone. In vivo biocontrol assays showed that the disease severity index (DSI) was significantly (P=0.05) reduced and mean fresh weight increased (P=0.001) in plantlets treated with strain S96 compared to those grown in the absence of the biocontrol strain. These findings indicate the potential of developing siderophore-producing Streptomyces endophytes for the biological control of fusarium wilt disease of banana.

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Antagonistic activity of endo‐β‐1,3‐glucanase from a novel isolate, Streptomyces sp. 9X166, against black rot in orchids

Sakdapetsiri

Fukuta

Aramsirirujiwet

et al. 2016

J. Basic Microbiol.

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A total of 123 actinomycetes was isolated from 12 varieties of wild orchids and screened for potential antagonistic activity against Phytophthora, which causes black rot disease in orchids. In vitro and in vivo experimental results revealed that Streptomyces sp. strain 9X166 showed the highest antagonistic activity; its β-1,3-glucanase production ability was a key mechanism for growth inhibition of the pathogen. PCR amplification and DNA sequencing of the 16S ribosomal RNA gene allowed the identification of this strain, with high similarity (99.93%) to the novel species Streptomyces similaensis. The glucanase enzyme, purified to homogeneity by anion exchange and gel filtration chromatography, showed a specific activity of 58 U mg(-1) (a 3.9-fold increase) and yield of 6.4%. The molecular weight, as determined by SDS-PAGE and gel filtration, was approximately 99 and 80 kDa, respectively, suggesting that the enzyme was a monomer. The purified enzyme showed the highest substrate specificity to laminarin, indicating that it was β-1,3-glucanase. The hydrolyzed products of cello-oligosaccharides suggested that this enzyme was endo-type β-1,3-glucanase. Streptomyces sp. 9X166 culture filtrate, possessing β-1,3-glucanase activity, could degrade both freeze-dried and living mycelium. This is the first report on a β-1,3-glucanase-producing Streptomyces sp. that could be an effective biocontrol agent for black rot disease in orchids.

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Glucanolytic Actinomycetes Antagonistic to Phytophthora fragariae var. rubi, the Causal Agent of Raspberry Root Rot

Cited by 137 publications

References 39 publications

Plant growth promotion and biological control ofPythium aphanidermatum, a pathogen of cucumber, by endophytic actinomycetes

Plant growth promotion and biological control ofPythium aphanidermatum, a pathogen of cucumber, by endophytic actinomycetes

Isolation and characterization of endophytic streptomycete antagonists of fusarium wilt pathogen from surface-sterilized banana roots

Antagonistic activity of endo‐β‐1,3‐glucanase from a novel isolate, Streptomyces sp. 9X166, against black rot in orchids

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