Glucocorticoid influence on porcine granulosa cell IGF-I and steroid hormone production in vitro

Viveiros, M.M.

doi:10.1016/s0093-691x(99)00050-3

Cited by 2 publications

(5 citation statements)

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“…In contrast, both basal and FSH-stimulated P 4 synthesis were unaffected, and indicate a specific effect on IGF-I action rather than an overall suppression of cell function. Similar findings were observed previously when granulosa cells were directly exposed to high levels of cortisol in vitro (Viveiros & Liptrap 1999). By day 18 (5 days post ACTH treatment) both overall P 4 and E 2 synthesis by granulosa cells were lower in the ACTH group.…”

Section: Figuresupporting

confidence: 89%

“…Treatment with high glucocorticoid concentrations during culture lowers FSH-stimulated E 2 synthesis, but enhances P 4 production by murine, porcine and bovine granulosa cells (Schoonmaker & Erickson 1983, Danisova et al 1987, Kawate et al 1993. Lower IGF-I production, and IGFstimulated steroid hormone synthesis by porcine granulosa cells, were also observed in response to culture with high levels of cortisol (Viveiros & Liptrap 1999). However, it was not apparent whether marked elevations of glucocorticoid hormones would elicit similar disruptions in vivo.…”

Section: Figurementioning

confidence: 98%

“…Recent work also demonstrated that porcine granulosa cells cultured with high levels of cortisol respond with lower insulin-like growth factor (IGF)-I synthesis and reduced IGF-I-stimulated P 4 production (Viveiros & Liptrap 1999).…”

Section: Introductionmentioning

confidence: 99%

“…If high glucocorticoid hormone levels disrupt ovarian IGF-I production and/or IGF-I action in vivo, as demonstrated with granulosa cell culture (Viveiros & Liptrap 1999), follicle development may be adversely affected. To assess this possibility, endogenous glucocorticoid concentrations were elevated by ACTH administration to gilts during the luteal phase of the oestrous cycle (days 9-13).…”

Section: Introductionmentioning

confidence: 99%

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ACTH treatment disrupts ovarian IGF-I and steroid hormone production

Viveiros

Liptrap²

2000

Journal of Endocrinology

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Hyper-adrenal activity and increased glucocorticoid hormone release are associated with disruptions in reproductive function and adverse effects on the ovary. The aim of this investigation was to determine whether elevated glucocorticoid hormone levels can influence ovarian IGF-I synthesis and action in vivo. To elevate endogenous glucocorticoid levels, gilts were treated with ACTH during the luteal phase of the oestrous cycle (days 9-13) while the control group received saline. The gilts were subsequently ovariectomized on either day 14 or day 18 of the oestrous cycle. Follicular fluid (FF) was collected from individual follicles; IGF-I and steroid hormone concentrations were determined by radioimmunoassay, and IGF-binding protein (IGFBP) expression was assessed by Western ligand blotting. Granulosa cells were also recovered and placed in culture to determine IGF-I, progesterone (P 4 ) and oestradiol-17 (E 2 ) production levels. The cells were cultured in serum-free medium for 5 days and supplemented with: (a) media alone, (b) IGF-I, (c) FSH and androstenedione (A 4 ), or (d) IGF-I with FSH and A 4 . The FF from ACTH-treated gilts was characterized by elevated (P<0·05) cortisol levels on day 14 and lower (P<0·05) E 2 values on both day 14 and day 18. Lower (P<0·05) IGF-I concentrations were also measured in the FF of ACTH-treated gilts collected on day 18. This altered hormone profile in FF was associated with impaired IGF-I and steroid hormone synthesis by granulosa cells. IGFstimulated P 4 production (P<0·01) by cells recovered from ACTH-treated gilts on day 14 was lower (P<0·05). By day 18, IGF-I, P 4 and E 2 production by cells from the ACTH group were all significantly (P<0·05) lower. These results demonstrate that increased glucocorticoid concentrations can disrupt subsequent ovarian IGF-I synthesis and IGF action in vivo and can, potentially, impair follicle maturation.

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Section: Figuresupporting

confidence: 89%

Section: Figurementioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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ACTH treatment disrupts ovarian IGF-I and steroid hormone production

Viveiros

Liptrap²

2000

Journal of Endocrinology

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“…As one of the contributing factors of ovulation, in ammatory mediators that their mRNA expressions were down-regulated in the BAA Group, do not seem to have enough in uence on ovulation and follicular growth in this group compare to ovarian hormones and metabolic status. Reduced ovulation rate and follicular growth observed in the GC group could be derived by: 1) Decrease in food intake that resulted in negative energy balance and consequently loss of live BW observed in the GC group, 2) induction of Insulin resistance [112], disturbance of IGF-I [113] and down-regulation of growth hormone [114], 3) observed down-regulation of estradiol and progesterone, and 4) observed reduction in mRNA expression of in ammatory mediators. Despite indicating the statistically similar size at the most classes of follicular growth, like the GC group, combinations groups had lower ovulation rate than the control, BAA, and BB .…”

Section: Ovarian and Body Functionsmentioning

confidence: 99%

The Combined Anti-inflammatory Strategy of Beta-2 Adrenergic Agonist and Glucocorticoid on the Laying Hen Model of Ovarian Cancer: the Immune Traits and Ovarian Inflammatory Functions

Hatefi

Shahneh

Pirsaraie

et al. 2021

Preprint

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Background: Ovarian cancer known as one of the most lethal gynecological malignancies mainly in older women, has been documented to link with chronic inﬂammation. Objective: This study was aimed to evaluate the combined strategy of glucocorticoid (GC) Fluticasone and beta-2 adrenergic agonist (BAA) Salmeterol on the ovarian inflammatory functions of the laying hen as a model of women ovarian cancer. Methods: White Leghorn hens aged 92 weeks were used for four weeks to be supplemented by individual of GC and BAA administration and their combination at three ratios GC:BAA 1:4 (GC+BAA1), 1:2 (GC+BAA2), and 1:1 (GC+BAA3), and beta blocker (BB) Propranolol. Ovulation rate and follicular growth were determined based on laying frequency and visual evaluation, respectively, the mRNA expressions of follicular beta-2 adrenergic receptor (β2ADR), cyclooxygenases (COX) 1 and 2, and cytokines were measured by real-time PCR. The plasma concentration of ovarian hormones cellular and humoral immune responses were measured via ELISA, neutrophil (heterophil) to lymphocyte ratio (NLR), and sheep red blood cell (SRBC) test, respectively. Results: As compared to control, combination groups of GC+BAA1 and 2 brought about a significant decrease in the mRNA expression of β2ADR, COX-2, and cytokines (P< 0.05). The ovulation rate was reduced in all combination ratios (P< 0.05). A signiﬁcant reduction was observed in the plasma estradiol content on GC+BAA groups (P< 0.05) and the content of progesterone and androgen were statistically similar in some of these groups. Although NLR was similar in GC+BAA2 and 3, these groups had more content in the whole immunoglobulin (Ig) and IgM (P< 0.05). The results indicated that body weight and food consummation were decreased in all of the combination groups (P< 0.05). Conclusion: The GC and BAA combination may result in a signiﬁcant reduction in some of the boosting factors on ovarian cancer, like ovulation intensity, pro-inflammatory mediators, and some of the ovarian steroid hormones that could define as the therapeutic approaches for the chronic inflammation-based carcinomas like ovarian cancer.

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Glucocorticoid influence on porcine granulosa cell IGF-I and steroid hormone production in vitro

Cited by 2 publications

References 0 publications

ACTH treatment disrupts ovarian IGF-I and steroid hormone production

ACTH treatment disrupts ovarian IGF-I and steroid hormone production

The Combined Anti-inflammatory Strategy of Beta-2 Adrenergic Agonist and Glucocorticoid on the Laying Hen Model of Ovarian Cancer: the Immune Traits and Ovarian Inflammatory Functions

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