Glucocorticoid Regulation of Androgen Binding Protein Expression in Primary Sertoli Cell Cultures from Rats

Lim, Kyu; Yoon, Suk-Joo; Lee, Myungsun; Byun, Sang-Hyun; Kweon, Gi‐Ryang; Kwak, Sang-Tae; Hwang, Byungil

doi:10.1006/bbrc.1996.0087

Cited by 16 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The observed glucocorticoid responsiveness of cultured Sertoli cells is in accordance with the presence of a GR in Sertoli cell cultures as shown in the present paper and in previous publications [15,33,34]. The GR levels measured in the present experiments (172 ± 49 fmol/mg protein) are quite similar to those (120 fmol/mg protein), which have previously been reported [15].…”

Section: Discussionsupporting

confidence: 95%

Transfection with steroid-responsive reporter constructs shows glucocorticoid rather than androgen responsiveness in cultured Sertoli cells

Denolet¹,

Gendt²,

Swinnen³

et al. 2006

The Journal of Steroid Biochemistry and Molecular Biology

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 95%

Transfection with steroid-responsive reporter constructs shows glucocorticoid rather than androgen responsiveness in cultured Sertoli cells

Denolet¹,

Gendt²,

Swinnen³

et al. 2006

The Journal of Steroid Biochemistry and Molecular Biology

View full text Add to dashboard Cite

“…842 Andrology, 2014, 2, 835-846 both spermatogenic cells and Sertoli cells express GR in a stagespecific pattern (Schultz et al, 1993;Biagini et al, 1995;Lim et al, 1996;Weber et al, 2000), an observation reproduced in our laboratory (Fig. S2); (ii) Adrenalectomy down-regulated GR expression and nuclear localization in both germ cells and Sertoli cells in adult rats (Biagini et al, 1995); (iii) hormonal replacement with corticosterone to ADX rats prevented the decrease in the population of step 7 spermatids induced by adrenalectomy (Saxena & Paul, 1988); and (iv) nanomolar concentrations of DEX induced the expression of the androgen binding protein mRNA, a gene involved in the hormonal regulation of spermatogenesis, in primary Sertoli cell culture (Lim et al, 1996).…”

Section: (H) (I) (C) (F) (D) (G)mentioning

confidence: 99%

“…S2); (ii) Adrenalectomy down-regulated GR expression and nuclear localization in both germ cells and Sertoli cells in adult rats (Biagini et al, 1995); (iii) hormonal replacement with corticosterone to ADX rats prevented the decrease in the population of step 7 spermatids induced by adrenalectomy (Saxena & Paul, 1988); and (iv) nanomolar concentrations of DEX induced the expression of the androgen binding protein mRNA, a gene involved in the hormonal regulation of spermatogenesis, in primary Sertoli cell culture (Lim et al, 1996). As immature spermatozoa leave the testis they are transported through the efferent ductules, reaching the epididymis, a highly convoluted tubule that is the site for sperm maturation and storage.…”

Section: (H) (I) (C) (F) (D) (G)mentioning

confidence: 99%

Impact of adrenalectomy and dexamethasone treatment on testicular morphology and sperm parameters in rats: insights into the adrenal control of male reproduction

et al. 2014

View full text Add to dashboard Cite

SUMMARYHere we investigated the hypothesis that normal levels of glucocorticoids, a class of adrenal steroid hormones, are required for normal testicular and epididymal functions. We examined the effects of the manipulation of glucocorticoid plasma levels by bilateral adrenalectomy (1, 2, 7 and 15 days) alone or in combination with daily treatment with the synthetic glucocorticoid dexamethasone (DEX; 5 lg/kg, i.p., 6 days) on the morphology of the testis and sperm parameters in rats. We showed that adrenalectomy led to a reduction in testicular sperm count and daily sperm production starting 2 days after surgery and a differential decrease in sperm count in the epididymis, according to the region and time post-adrenalectomy analysed. In parallel, testes from 7-day adrenalectomized (ADX) rats displayed a higher frequency of damaged seminiferous tubules and the presence of elongated spermatids retained in the basal epithelial compartment in stages IX-XVII, which is indicative of defective spermiation. The alkaline comet assay revealed a late effect of adrenalectomy on epididymal sperm DNA fragmentation, which was increased only 15 days after surgery. DEX treatment prevented the changes in testicular and epididymal sperm count observed in 7-day ADX rats, but failed to protect the testis from ADX-induced morphological abnormalities. Thus, our results indicated that glucocorticoids may be involved in events related to the maintenance of spermatogenesis and sperm maturation during adulthood. These findings provide new insights into the importance of adrenal steroids to male fertility.

show abstract

“…In this regard, it is worthwhile to recall the work of Lim et al (1996), who showed a marked decrease in the mRNA expression of an- drogen binding protein (ABP) in Sertoli cells exposed to higher doses of DEX (1 and 10 mmol/L).…”

Section: Discussionmentioning

confidence: 97%

Corticosterone impairs the mRNA expression and activity of 3β- and 17β-hydroxysteroid dehydrogenases in adult rat Leydig cells

Badrinarayanan

Srinivasan

Nithya

et al. 2006

Biochem. Cell Biol.

View full text Add to dashboard Cite

Clinical and experimental studies, including our own observations, have shown the adverse effects of excess glucocorticoids on testicular steroid hormone production. The present study was designed to gain insight into the molecular mechanisms by which excess corticosterone impairs Leydig cell steroidogenesis. To achieve this, adult rats were administered with corticosterone-21-acetate (2 mg/100 g body weight) twice daily for 15 days. After the treatment period, rats were killed by decapitation. The testes were removed, decapsulated aseptically and used for the isolation of Leydig cells. Purified Leydig cells were used for assessing the activity of 3beta- and 17beta-hydroxysteroid dehydrogenases (HSDs) and total RNA isolation. For in vitro studies, purified Leydig cells (7.5 x 10(6) cells) of control rats were plated in culture flasks and exposed to different concentrations (50, 100, 200, 400, and 800 nmol/L) of corticosterone for 24 h. At the end of incubation, total RNA was isolated from cultured Leydig cells, and the mRNA of 3beta- and 17beta-HSDs was quantified by RT-PCR. A significant reduction in the activities and levels of 3beta-HSD type-I and 17beta-HSD type-III mRNAs in Leydig cells were observed. In vitro studies demonstrated a dose-dependent significant impairment in both the activity and mRNA expression of these enzymes. These results suggest that corticosterone might have a direct effect on the transcription of the genes of 3beta- and 17beta-HSD. It is inferred from the present in vivo and in vitro studies that one of the molecular mechanisms by which excess corticosterone decreases the steroidogenic potency of Leydig cells is by suppressing the mRNA expression of 3beta-HSD type-I and 17beta-HSD type-III enzymes.

show abstract

Glucocorticoid Regulation of Androgen Binding Protein Expression in Primary Sertoli Cell Cultures from Rats

Cited by 16 publications

References 0 publications

Transfection with steroid-responsive reporter constructs shows glucocorticoid rather than androgen responsiveness in cultured Sertoli cells

Transfection with steroid-responsive reporter constructs shows glucocorticoid rather than androgen responsiveness in cultured Sertoli cells

Impact of adrenalectomy and dexamethasone treatment on testicular morphology and sperm parameters in rats: insights into the adrenal control of male reproduction

Corticosterone impairs the mRNA expression and activity of 3β- and 17β-hydroxysteroid dehydrogenases in adult rat Leydig cells

Contact Info

Product

Resources

About