Glucocorticoids Increase Pulmonary S-Adrenergic Receptors in Fetal Rabbit

Cheng, John B.; Goldfien, Alan; Ballard, Philip L.; Roberts, James M.

doi:10.1210/endo-107-5-1646

Cited by 136 publications

(60 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The modulation of this cascade by influences such as glucocorticoids and thyroid hormone is also of great interest in view of the known effects of hormonal manipulation on the surfactant system in human lung as well as in lung of other species (32) and on the development of P-adrenergic receptors in lung of other species (33)(34)(35).…”

Section: Resultsmentioning

confidence: 99%

β-Adrenergic Receptors and Cyclic Adenosine Monophosphate Generation in Human Fetal Lung

et al. 1987

View full text Add to dashboard Cite

ABSTRACT. We designed experiments to determine whether P-adrenergic receptors are present and functional in human fetal lung during the 2nd trimester of gestation. To determine the presence of p receptors, characterize their binding sites, and assess changes in receptor with gestational age, we performed radioligand binding assays with the specific, high-affinity P antagonist, '251-iodocyanopindolol, in membrane particulates from the lungs of 2nd trimester abortuses (15-23 wk). Binding of '251-iodocyanopindolol was saturable and of high affinity (dissociation constant = 40 pM). Binding was stereoselective as determined by competition studies with (-) and (+) stereoisomers of propranolol. Agonist affinities (isoproterenol > epinephrine >> norepinephrine) were consistent with a predominance of 8-2 receptors; this predominance was confirmed by competition studies with the specific P-2 receptor antagonist ICI 118-551 (75% 8-2,25% P-1). The concentration of B-adrenergic receptors increased with gestational age. To assess the functional coupling of the fi receptors, we tested the ability of receptor occupancy to activate adenylate cyclase. For this assay, we incubated minced human fetal lung with P agonists and determined the amount of cAMP generated. P Agonists stimulated cAMP generation more than 2-fold. We conclude that Padrenergic receptors are present and functional in human fetal lung as early as the 2nd trimester. (Pediatr Res 21:142-147,1986) Abbreviations ICYP, '2~-iodocyanopindolol ATP, adenosine triphosphate CAMP, cyclic adenosine monophosphate Gpp(NH)p, 5'-guanylylimidodiphosphate GTP, guanosine triphosphate K,,, dissociation constant There is increasing evidence for an integral role of adrenergic responses in the complex transition which the fetal lungs undergo in preparation for extrauterine life. Indirect evidence comes from clinical situations in which infants are not exposed to high levels of the adrenergic catecholamines, epinephrine and norepinephrine. For example, infants born by cesarean section without labor Received March 26, 1986: accepted September 18, 1986 have lower concentrations of catecholamines in amniotic fluid and cord blood than do those delivered vaginally or by cesarean section after labor (I). In association with these lower catecholamine levels, infants born by cesarean section without labor have a higher incidence of breathing difficulties (2), less mature biochemical indices of lung maturity (3,4), and lower tidal volumes and lung compliance (1) than do other infants. In contrast, infants born to mothers who received P-adrenergic agonists immediately before delivery are reported to have a lower incidence of hyaline membrane disease (5).In other species, there is substantial experimental evidence that P-adrenergic responses are involved in fetal lung maturation (6). Administration of P-adrenergic agonists to fetal sheep and rabbits significantly decreases lung water content (7,8), increases surfactant release (9, lo), and increases lung stability (1 1, 12). In fetal rabbits,...

show abstract

Section: Resultsmentioning

confidence: 99%

β-Adrenergic Receptors and Cyclic Adenosine Monophosphate Generation in Human Fetal Lung

et al. 1987

View full text Add to dashboard Cite

show abstract

“…P-Adrenergic receptor sites increase during the perinatal period in the rat and rabbit lung and these increases are apparently mediated by both corticosteroids (prenatally) and thyroxine (postnally) in the developing lung (4,10,(20)(21)(22). There is evidence that developmental changes in acetylcholinesterase-positive pulmonary cells are also hormonally regulated, being enhanced by thyroxine in vitro (1 5).…”

Section: Discussionmentioning

confidence: 99%

Muscarinic Cholinergic Receptors in Developing Rat Lung

Whitsett

Hollinger

1984

Pediatr Res

View full text Add to dashboard Cite

“…For other catecholamine receptors such as beta-adrenergic receptors, corticosteroids have significant effects on maturation, 41 but the effects are species specific. Rabbit and rat fetuses exposed to corticosteroids in utero increase pulmonary beta-receptor density, 42 but this does not occur in fetal sheep. 43 If the increase in DA D 1 -R mRNA in rabbit adrenal glands was followed by an increase in receptor density, as is the case for rabbit lungs, one might suspect a positive effect of corticoids.…”

Section: Discussionmentioning

confidence: 91%

Effect of betamethasone on the expression of dopamine D 1 receptor mRNA in the developing rabbit adrenal gland

Labaune¹,

Boutroy²,

Bairam³

2003

Current Therapeutic Research

View full text Add to dashboard Cite

Background: Antenatal corticotherapy is widely used to enhance pulmonary fetal maturation in cases in which premature birth is likely. The adrenal gland, which has a key role in controlling fetal and neonatal adaptation, appears to be a target organ of glucocorticoids.Objectives: The aim of this study was first to determine the ontogenic profile of dopamine D 1 receptor (DA D 1 -R) messenger RNA (mRNA) in the rabbit. The effects of antenatal exposure to exogenous corticoids on levels of adrenal DA D 1 -R mRNA expression were examined.Methods: Pregnant rabbits free of any treatment or handling before delivery were chosen for the study of DA D 1 -R mRNA ontogenic profile. For the study of the antenatal exposure to exogenous corticoids, pregnant rabbits were given 2 injections of either betamethasone 0.1 mg/kg or saline 0.1 mL/kg. DA D 1 -R mRNA expression was determined using northern blot analysis at 4 developmental ages: fetus (at 27 days of gestation), 1 day of age, 25 days of age, and adulthood. Rabbits were allocated to their respective group (treated or untreated) depending on maternal treatment (betamethasone or saline, respectively).Results: Four pregnant rabbits were used for the ontogenic-profile group, which comprised 11 fetuses, seven 1-day-old rabbits, four 25-day-old rabbits, and 2 adults. Six other pregnant rabbits received betamethasone; 6 saline. The treated group comprised 12 fetuses, twelve 1-day-old rabbits, four 25-day-old rabbits, and 3 adults. The untreated group comprised 12 fetuses, fifteen 1-dayold rabbits, five 25-day-old rabbits, and 3 adults. DA D 1 -R mRNA was expressed in rabbit adrenal glands from the fetal period to adulthood and this expression was not age dependent. Moreover, antenatal corticotherapy induced a significant increase in respective DA D 1 -R mRNA levels of 20%, 15%, and 8% in treated fetuses, 1-day-old rabbits, and 25-day-old rabbits compared with the untreated groups (P Ͻ 0.003, 0.003, and 0.005, respectively). This increase was not observed in adulthood.

show abstract

Glucocorticoids Increase Pulmonary S-Adrenergic Receptors in Fetal Rabbit

Cited by 136 publications

References 0 publications

β-Adrenergic Receptors and Cyclic Adenosine Monophosphate Generation in Human Fetal Lung

β-Adrenergic Receptors and Cyclic Adenosine Monophosphate Generation in Human Fetal Lung

Muscarinic Cholinergic Receptors in Developing Rat Lung

Effect of betamethasone on the expression of dopamine D 1 receptor mRNA in the developing rabbit adrenal gland

Contact Info

Product

Resources

About