Glucocorticoids induce transcription of ribosomal protein genes in rat liver

Flusser, Gideon; Ginzburg, V. P.; Meyuhas, Oded

doi:10.1016/0303-7207(89)90148-2

Cited by 13 publications

(11 citation statements)

References 38 publications

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“…Mouse Ltk-cells were grown in monolayer as described previously (21). For labeling the RNA, a confluent culture in 100-mm dishes was incubated for 2 h at 37°C with [5,6- 25 mM NaCl, 0.05% Triton X-100, 100 ,ug of heparin per ml).…”

Section: Methodsmentioning

confidence: 99%

“…Labeled RNA was extracted from the reaction mixture (25) and hybridized to GeneScreen Plus (New England Nuclear)-bound DNA. Immobilization of plasmid DNAs to the filter was carried out as previously described (21), using a slot blot apparatus (Schleicher & Schuell). Plasmid-bearing filters were prehybridized at 65°C for overnight with 50 mM N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES; pH 7.5), 0.5 M NaCl, 0.02% Ficoll, 0.02% polyvinylpyrrolidone, 10 (40); and 0.4-kb EcoRI fragment containing the 5' end of mouse eIF-4A cDNA (57).…”

Section: Methodsmentioning

confidence: 99%

“…Posttranscriptional control of rp genes, most probably through stability of the corresponding transcripts, has been reported to occur in anucleate Xenopus embryos (62) and in dexamethasone-treated rats (21). In addition, control at the RNA processing level is evident for rpL1 in Xenopus laevis (10).…”

mentioning

confidence: 99%

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Selective translational control and nonspecific posttranscriptional regulation of ribosomal protein gene expression during development and regeneration of rat liver.

Aloni¹,

Peleg²,

Meyuhas³

1992

Mol. Cell. Biol.

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Mammalian liver development is accompanied by a transition from rapid growth in the fetus to a quiescent state in the adult. However, extensive proliferation can be induced in the adult liver by partial hepatectomy. In this study, we examined the regulation of ribosomal protein (rp) gene expression in the developing and regenerating rat liver. Our results indicate that the translation of rp mRNAs is selectively repressed by about 70%o upon development from fetal to adult life, as illustrated by the decrease in ribosomal loading. In addition, the relative abundance of these mRNAs, like that of several other, but not all, housekeeping mRNAs, declines during development through a posttranscriptional mechanism. When liver cells commence growth following partial hepatectomy, translation of rp mRNAs is resumed to near-maximal capacity, as judged by their very efficient recruitment into polysomes. The concomitant increase in the abundance of rp mRNAs under these circumstances is achieved by a posttranscriptional mechanism. The apparent fluctuations in the translation efficiency of rp mRNAs are accompanied by parallel changes in the expression of the genes encoding the initiation factors eIF-4E and eIF-4A. Our results indicate that selective translational control of rp mRNAs in mammals is not confined to manipulated cells in culture but constitutes an important regulatory mechanism operating in vivo in the course of liver development and regeneration.The biosynthesis of ribosomes in vertebrates is primarily coordinated with the cellular growth status and requires an equimolar accumulation of four rRNA and about 80 different ribosomal protein (rp) molecules. This stoichiometry is maintained by coordinate regulation at various levels of gene expression from transcription to protein turnover (4,31,43,49). Clearly, the translational control of rp mRNAs is the most prevalent regulatory mechanism of vertebrate rp gene expression and operates under a variety of physiological conditions (reference 41 and references therein). Recently, we have shown that the 5'-terminal pyrimidine tract, adjacent to the cap site of all vertebrate rp mRNAs rigorously analyzed thus far, plays a critical role in their coordinate translational control (41).Posttranscriptional control of rp genes, most probably through stability of the corresponding transcripts, has been reported to occur in anucleate Xenopus embryos (62) and in dexamethasone-treated rats (21). In addition, control at the RNA processing level is evident for rpL1 in Xenopus laevis (10). Finally, a balanced accumulation of ribosomal proteins (r-proteins) is also achieved by modulating their turnover, as observed in mouse oocytes (39) and differentiating rat myoblasts (30).The development of rodent liver is associated with progressive decrease of mitotic activity to almost undetectable levels in the adult tissue (reference 46 and references therein). Concomitantly, the hepatocytes acquire differentiated functions and lose others. These developmental changes involve extensive alterations...

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Selective translational control and nonspecific posttranscriptional regulation of ribosomal protein gene expression during development and regeneration of rat liver.

Aloni¹,

Peleg²,

Meyuhas³

1992

Mol. Cell. Biol.

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“…In yeast, most of the ribosomal protein gene promoters contain one or two sites for a global regulator, RAP1p (Klein and Struhl 1994;Kraakman et al, 1993), and the transcription of ribosomal proteins is coordinated with rRNA synthesis (Warner, 1989). Conserved motifs where also found in chicken (Maeda et al, 1993) or mouse (Genuario et al, 1993) ribosomal protein genes, and a coregulation of a set of ribosomal proteins and rRNA was already described in rat liver after a glucocorticoid stimulation (Flusser et al, 1989). As we know that these genes are very conserved throughout species, the existence of such a global regulator or common motif of regulation probably exists in the rat genome.…”

Section: Ribosomal Protein and Translationally Controlled Tumor Protementioning

confidence: 99%

Differentially expressed genes in C6.9 glioma cells during vitamin D-induced cell death program

et al. 1998

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C6.9 rat glioma cells undergo a cell death program when exposed to 1,25-dihydroxyvitamin D3 (1,25-D3). As a global analytical approach, we have investigated gene expression in C6.9 engaged in this cell death program using differential screening of a rat brain cDNA library with probes derived from control and 1,25-D3-treated cells. Using this methodology we report the isolation of 61 differentially expressed cDNAs. Forty-seven cDNAs correspond to genes already characterized in rat cells or tissues. Seven cDNAs are homologous to yeast, mouse or human genes and seven are not related to known genes. Some of the characterized genes have been reported to be differentially expressed following induction of programmed cell death. These include PMP22/gas3, MGP and b-tubulin. For the first time, we also show a cell death program induced up-regulation of the c-myc associated primary response gene CRP, and of the proteasome RN3 subunit and TCTP/mortalin genes. Another interesting feature of this 1,25-D3 induced-cell death program is the down-regulated expression of transcripts for the microtubule motor dynein heavy chain/MAP 1C and of the calcium-binding S100b protein. Finally 15 upregulated cDNAs encode ribosomal proteins suggesting a possible involvement of the translational apparatus in this cell program. Alternatively, these ribosomal protein genes could be up-regulated in response to altered rates of cellular metabolism, as has been demonstrated for most of the other isolated genes which encode proteins involved in metabolic pathways. Thus, this study presents to our knowledge the first characterization of genes which are differentially expressed during a cell death program induced by 1,25-D3. Therefore, this data provides new information on the fundamental mechanisms which participate in the antineoplastic effects of 1,25-D3 and on the machinery of a cell death program in a glioma cell line.

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“…Удельное содержание РНК в гепатоцитах более чем в 20 раз превышает аналогичный показатель в тимоцитах (Schmidt, Schibler, 1995). Также следует отметить, что воздействие глюкокортикоидами приводит к увеличению удельного количества РНК в печени крыс (Thompson et al, 1976;Flusser et al, 1989). В то же время в мозге, ти-мусе, а также ряде других тканей у млекопитающих под влиянием глюкокортикоидов происходит не увеличение, а уменьшение удельного количества РНК (Zimmerman et al, 1970).…”

Section: глубина секвенирования транскриптома протяженность транскриunclassified

The design of experiments for the transcriptome studies by high-throughput sequencing methods

Menshanov¹,

Дыгало²

2016

Vestn. VOGiS

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Glucocorticoids induce transcription of ribosomal protein genes in rat liver

Cited by 13 publications

References 38 publications

Selective translational control and nonspecific posttranscriptional regulation of ribosomal protein gene expression during development and regeneration of rat liver.

Selective translational control and nonspecific posttranscriptional regulation of ribosomal protein gene expression during development and regeneration of rat liver.

Differentially expressed genes in C6.9 glioma cells during vitamin D-induced cell death program

The design of experiments for the transcriptome studies by high-throughput sequencing methods

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