Glucocorticoids reduce alveolar and trabecular bone in mice

Bouvard, Béatrice; Gallois, Yves; Legrand, Érick; Audran, Maurice; Chappard, Daniel

doi:10.1016/j.jbspin.2012.01.009

Cited by 28 publications

(27 citation statements)

References 40 publications

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“…The maximum score of 25 was registered for a report on the maxillar bone [35], while a minimum sore of 13 was found in a mandibular study [44]. The median score was 21 and was reached by 11 studies: 1 in the maxilla [6], 6 in the mandible [11], [13], [31], [32], [34], [42] and 4 in the condyle [22], [25], [30], [33].…”

Section: Resultsmentioning

confidence: 99%

“…Six studies applied in vivo micro-CT, performed in the maxilla [6], [15], [20], [21] and in the condyle [10], [22]. The scanning device which was most often used was the μCT 40 (Scanco Medical, Brüttisellen, Switzerland) [9], [11], [13], [14], [19], [23], [24], [25], [26], [27], [28], [29], [30], [31], followed by the Skyscan apparatus (Skyscan, Aartselaar, Belgium) [3], [4], [8], [20], [21], [32], [33], [34], [35]. The results of the quantitative analysis are presented in Table 2, Table 3, Table 4.…”

Section: Resultsmentioning

confidence: 99%

“…A total of 10 papers investigated the effect of mechanical loading, through diet texture modification and mastication [7], [11], [13], [19], [27], [29], [31], [36], [37], [38] on the bone micro-architecture, while 8 addressed the effect of drug intervention [3], [12], [32], [34], [39], [40], [41], [42]. One paper investigated the mandibular bone architecture in the ovariectomy-induced osteoporotic animal [43].…”

Section: Resultsmentioning

confidence: 99%

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Micro-CT analysis of the rodent jaw bone micro-architecture: A systematic review

et al. 2015

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IntroductionKnowledge about macro- and micro-structural characteristics may improve in vivo estimation of the quality and quantity of regenerated bone tissue. For this reason, micro-CT imaging has been applied to evaluate alveolar bone remodelling, alterations of periodontal ligament thickness and cortical and trabecular bone changes in rodent jaw bones. In this paper, we provide a systematic review on the available micro-CT literature on jaw bone micro-architecture.MethodologyA detailed search through the PubMed database was performed. Articles published up to December 2013 and related to maxilla, mandible and condyle with quantitatively analysed bone micro-architectural parameters were considered eligible for inclusion. Two reviewers assessed the search results according to inclusion criteria designed to identify animal studies quantifying the bone micro-architecture of the jaw rodent bones in physiological or drug-induced disease status, or in response to interventions such as mechanical loading, hormonal treatment and other metabolic alterations. Finally, the reporting quality of the included publications was evaluated using the tailored ARRIVE guidelines outlined by Vignoletti and Abrahamsson (2012).ResultsDatabase search, additional manual searching and assessment of the inclusion and exclusion criteria retrieved 127 potentially relevant articles. Eventually, 14 maxilla, 20 mandible and 12 condyle articles with focus on bone healing were retained, and were analysed together with 3 methodological papers. Each study was described systematically in terms of subject, experimental intervention, follow-up period, selected region of interest used in the micro-CT analysis, parameters quantified, micro-CT scanner device and software. The evidence level evaluated by the ARRIVE guidelines showed high mean scores (between 18 and 25; range: 0–25), indicating that most of the selected studies are well-reported. The major obstacles identified were related to sample size calculation, absence of adverse event descriptions, randomization or blinding procedures.ConclusionsThe evaluated studies are highly heterogeneous in terms of research topic and the different regions of interest. These results illustrate the need for a standardized methodology in micro-CT analysis. While the analysed studies do well according to the ARRIVE guidelines, the micro-CT procedure is often insufficiently described. Therefore we recommend to extend the ARRIVE guidelines for micro-CT studies.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Micro-CT analysis of the rodent jaw bone micro-architecture: A systematic review

et al. 2015

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“…We observed that GC treated mice had decreased bone volume, bone strength in the distal femur and vertebra, reduced bone formation, and evidence of reduced mineralizing surface and surface based bone formation [17,30,36]. Yao et al reported that GC-induced bone loss is caused by early upregulation of genes involved in osteoclast activation, function, and adipogenesis, followed by a delayed but extended reduction in expression of genes associated with osteoblast activation and maturation [28].…”

Section: Discussionmentioning

confidence: 99%

A Novel Hybrid Compound LLP2A-Ale Both Prevented and Rescued the Osteoporotic Phenotype in a Mouse Model of Glucocorticoid-Induced Osteoporosis

et al. 2016

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Prolonged glucocorticoid (GC) administration causes secondary osteoporosis (GIOP) and non-traumatic osteonecrosis. LLP2A-Ale is a novel bone seeking compound that recruits mesenchymal stem cells to the bone surface, stimulates bone formation, and increases bone mass. The purpose of this study was to determine if treatment with LLP2A-Ale alone or in combination with parathyroid hormone (PTH) could prevent or treat GIOP in a mouse model. Four month old male Swiss-Webster mice were randomized to a prevention study with placebo, GC (day 1 – 28), and GC + LLP2A-Ale (IV, day 1) or a treatment study with placebo, GC (day 1 – 56), GC + LLP2A-Ale (IV, day 28), GC + PTH, and GC + LLP2A-Ale + PTH (day 28 – 56). Mice were sacrificed on day 28 (prevention study) or on day 56 (treatment study). The study endpoints included bone mass, bone strength, serum markers of bone turnover (P1NP and CTX-I) and angiogenesis (VEGF-A), surface-based bone turnover, and blood vessel density. LLP2A-Ale prevented GC-induced bone loss and increased mechanical strength in the vertebral body (day 28 and 56) and femur (day 56). LLP2A-Ale, PTH, and LLP2A-Ale + PTH treatment significantly increased the mineralizing surface, bone formation rate, mineral apposition rate, double labeled surface and serum P1NP level on day 56. LLP2A-Ale and PTH treatment increased femoral blood vessel density and LLP2A-Ale increased serum VEGF-A on day 28. Therefore, LLP2A-Ale monotherapy could be a potential option to both prevent and treat GC-induced osteoporosis and bone fragility.

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“…14 Following analyses in vitro, other animal studies have reported that corticosteroids induced bone resorption. 15 A human cross-sectional study evaluating the long-term effects of inhaled corticosteroids reported that corticosteroids might reduce bone remodeling, causing a decrease in bone mineral density. 16 Another side effect associated with taking multiple drugs is xerostomia, which is associated with inflammation and bleeding from periodontal structures.…”

Section: Introductionmentioning

confidence: 99%

Is there a relationship between periodontal conditions and number of medications among the elderly?

Natto¹,

Aladmawy²,

Alshaeri³

et al. 2016

Ghana Medical Journal

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SUMMARYObjective: To investigate possible correlations of clinical attachment level and pocket depth with number of medications in elderly individuals. Methods: Intra-oral examinations for 139 patients visiting Tufts dental clinic were done. Periodontal assessments were performed with a manual UNC-15 periodontal probe to measure probing depth (PD) and clinical attachment level (CAL) at 6 sites. Complete lists of patients' medications were obtained during the examinations. Statistical analysis involved Kruskal-Wallis, chi square and multivariate logistic regression analyses. Results: Age and health status attained statistical significance (p< 0.05), in contingency table analysis with number of medications. Number of medications had an effect on CAL: increased attachment loss was observed when 4 or more medications were being taken by the patient. Number of medications did not have any effect on periodontal PD. In multivariate logistic regression analysis, 6 or more medications had a higher risk of attachment loss (>3mm) when compared to the no-medication group, in crude OR (1.20, 95% CI:0.22-6.64), and age adjusted (OR=1.16, 95% CI:0.21-6.45), but not with the multivariate model (OR=0.71, 95% CI:0.11-4.39). Conclusion: CAL seems to be more sensitive to the number of medications taken, when compared to PD. However, it is not possible to discriminate at exactly what number of drug combinations the breakdown in CAL will happen. We need to do further analysis, including more subjects, to understand the possible synergistic mechanisms for different drug and periodontal responses.

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Glucocorticoids reduce alveolar and trabecular bone in mice

Abstract: Although the amount of alveolar bone is very low in the mouse, this study shows that GCs can induce an alveolar bone loss in long-term treated animals.

Cited by 28 publications

References 40 publications

Micro-CT analysis of the rodent jaw bone micro-architecture: A systematic review

Micro-CT analysis of the rodent jaw bone micro-architecture: A systematic review

A Novel Hybrid Compound LLP2A-Ale Both Prevented and Rescued the Osteoporotic Phenotype in a Mouse Model of Glucocorticoid-Induced Osteoporosis

Is there a relationship between periodontal conditions and number of medications among the elderly?

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