Gluconate production by spores of Aspergillus niger

Moksia, Jean; Larroche, Christian; Gros, Jean‐Bernard

doi:10.1007/bf00129725

Cited by 23 publications

(13 citation statements)

References 13 publications

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“…Production of gluconic acid in fungi, such as P. variabile [8] and our isolate P. rugulosum IR-94MF1, is a trait that is related to the Mps phenotype as already found in Gram-negative bacteria [7,8]. This organic acid has also been found in several saprophytic fungi such as P. chrysogenum [15], P. notatum [16], Aspergillus niger [17] and the ectomycorrhizal species Tricholoma robustum [18]. Gluconic acid is produced extracellularly or in the perisplasmic space of the cells of some ¢lamentous fungi by a direct oxidation of aldonic sugars.…”

supporting

confidence: 75%

Characteristics of phosphate solubilization by an isolate of a tropical Penicillium rugulosum and two UV-induced mutants

Reyes

Bernier

Simard

et al. 1999

FEMS Microbiology Ecology

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After screening several fungal isolates obtained from Venezuelan soils, the isolate IR‐94MF1 of Penicillium rugulosum was selected for its high mineral phosphate solubilizing activity (Mps+) with hydroxyapatite. Mutants with altered (Mps−) or amplified activity (Mps++) were obtained by UV irradiation of conidia. When glucose was used as the sole C source by the fungus, gluconic acid was associated with the hydroxyapatite activity. In solid media, in comparison to glucose and maltose, sucrose appeared to be the best C source for the solubilization of hydroxy‐apatite and FePO4.

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supporting

confidence: 75%

Characteristics of phosphate solubilization by an isolate of a tropical Penicillium rugulosum and two UV-induced mutants

Reyes

Bernier

Simard

et al. 1999

FEMS Microbiology Ecology

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“…Due to enormous demand of about 50 000-60 000 tons per annum, the microbial fermentation processes are exclusively used for commercial gluconic acid production using glucose as the major carbohydrate source (Roehr et al 1996;Roukas 2000;Singh et al 2003). Gluconic acid and its derivatives are mainly obtained by submerged fermentation using variety of Aspergillus niger strains with higher product yield at defined parameters (Moksia et al 1996;Roehr et al 1996;Roukas 2000). Solid-state culture condition has been demonstrated as the promising system of fermentation for the further economizing the process of fermentation (Torres et al 1998;Pandey et al 1999;Singh et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Bioconversion of grape must into modulated gluconic acid production by Aspergillus niger ORS-4.410

Singh

2006

J Appl Microbiol

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Aims: Analysis of regulators for modulated gluconic acid production under surface fermentation (SF) condition using grape must as the cheap carbohydrate source, by mutant Aspergillus niger ORS‐4·410. Replacement of conventional fermentation condition by solid‐state surface fermentation (SSF) for semi‐continuous production of gluconic acid by pseudo‐immobilization of A. niger ORS‐4·410. Methods and Results: Grape must after rectification was utilized for gluconic acid production in batch fermentation in SF and SSF processes using mutant strain of A. niger ORS‐4·410. Use of rectified grape must led to the improved levels of gluconic acid production (80–85 g l−1) in the fermentation medium containing 0·075% (NH4)2HPO4; 0·1% KH2PO4 and 0·015% MgSO4·7H2O at an initial pH 6·6 (±0·1) under surface fermentation. Gluconic acid production was modulated by incorporating the 2% soybean oil, 2% starch and 1% H2O2 in fermentation medium at continuously high aeration rate (2·0 l min−1). Interestingly, 95·8% yield of gluconic acid was obtained when A. niger ORS‐4·410 was pseudo‐immobilized on cellulose fibres (bagasse) under SSF. Four consecutive fermentation cycles were achieved with a conversion rate of 0·752–0·804 g g−1 of substrate into gluconic acid under SSF. Conclusions: Use of additives modulated the gluconic acid production under SF condition. Semi‐continuous production of gluconic acid was achieved with pseudo‐immobilized mycelia of A. niger ORS‐4·410 having a promising yield (95·8%) under SSF condition. Significance and Impact of the Study: The bioconversion of grape must into modulated gluconic acid production under SSF conditions can further be employed in fermentation industries by replacing the conventional carbohydrate sources and expensive, energy consuming fermentation processes.

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“…Most of the enzymes produced by the fungus are secreted extracellularly, though some quantity of intracellular enzymes might also be present (Wolken et al 2003). As the fungus continues its growth on the solid fermentation substrate, the mycelial mat gradually disappears giving rise to the spores, and in some fungi, the extracellular products previously secreted by the mycelium is transported to the spores, and stored as an intracellular product (Moksia et al 1996;Ramachandran et al 2007). Many methods of microbial cell disruption have been described for fungi.…”

Section: Introductionmentioning

confidence: 98%

Exploration of fungal spores as a possible storehouse of proteolytic biocatalysts

Sumantha

Fontanille

Larroche

et al. 2008

World J Microbiol Biotechnol

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Studies were carried out to define the relation between enzyme production and fungal sporulation, in solid-state cultivation conditions of the filamentous fungus Aspergillus oryzae NRRL 2217 to get information on possible links between metabolite synthesis and differentiation phenomena. The efforts taken to explore the possibility for the presence of a neutral protease inside the spores of this fungus was to increase the overall enzyme yield. Results showed that the production of enzyme (neutral protease) and biomass (total protein) were synchronised, both reaching their respective maximum levels at 48 h of fermentation, and decreasing thereafter. Neutral protease synthesis was not related to sporulation. The spores produced were subjected to various permeabilisation procedures, and the increase in the levels of neutral protease was monitored. Mechanical shear was the sole technique that was able to disrupt spores but even this failed to increase enzyme titres, confirming the absence of intra-spore proteases.

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Gluconate production by spores of Aspergillus niger

Cited by 23 publications

References 13 publications

Characteristics of phosphate solubilization by an isolate of a tropical Penicillium rugulosum and two UV-induced mutants

Characteristics of phosphate solubilization by an isolate of a tropical Penicillium rugulosum and two UV-induced mutants

Bioconversion of grape must into modulated gluconic acid production by Aspergillus niger ORS-4.410

Exploration of fungal spores as a possible storehouse of proteolytic biocatalysts

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