Gluconic acid produced by Gluconacetobacter diazotrophicus Pal5 possesses antimicrobial properties

Nieto-Peñalver, Carlos Gabriel; Savino, María J.; Bertini, Elisa Violeta; Sánchez, Leandro Arturo; Figueroa, Lucía I. C. de

doi:10.1016/j.resmic.2014.06.003

Cited by 26 publications

(21 citation statements)

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“…Gluconic acid, which comes from the oxidation of glucose (Nieto‐Penalver, Savino, Bertini, Sanchez, & De Figueroa, ), exhibited the same distribution as the oxylipins. This result implies that, apart from antioxidants, the fungus can also prevent injury owing to reactive oxygen species through the autoxidation of glucose.…”

Section: Resultsmentioning

confidence: 97%

Metabolomic variation in wild and cultured cordyceps and mycelia of Isaria cicadae

Zhang

Fan

et al. 2019

Biomedical Chromatography

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Isaria cicadae is one of the fungi used in traditional Chinese medicine with the longest tradition. It is used not only as a herbal medicine but also as a health food in Asia, together with cultured cordyceps and mycelia of the fungus used as substitute. However, the differences in their metabolite are unknown. Using a high‐performance liquid chromatography–mass spectrometry (HPLC–MS)‐based metabolomic method, we found that the fungus varies in its metabolism during growth on wild insects, artificially raised insects and artificial medium. There were 109 discriminatory metabolites detected in the samples by orthogonal projection to latent structure discriminant analysis and one‐way ANOVA. High level of nonribosomal peptides (NRPs) only existed in the insect portions of the wild cordyceps (WI) and cultured cordyceps (CI), revealing that immunostimulation of the host insects enhanced the synthesis of NRPs in the fungus. The finding of a significantly higher level of sphingolipids in both the insect portions (WI, CI) and the coremia of the wild cordyceps (WC) and cultured cordyceps (CC) but not in cultured mycelia (CM) of I. cicadae implies that the immunostimulation of the live insects can induce the fungus to produce more sphingolipids, and this enhanced ability is probably heritable. Apart from NRPs and sphingolipids, the insect portions also contained higher levels of bioactive compounds such as lateritin, anisomycin, streptimidone and ustiloxins. In contrast, the coremium groups (WC, CC) and CM contained 10‐fold less NRP but much higher levels of sanative metabolites such as tocotrienol, 3′‐deoxy‐hanasanagin, γ‐aminobutyric acid and phospholipids than the insect portions. The significantly higher content of antioxidants in WC, CC and CM than in WI and CI suggests that environmental oxygen has a significant effect on the metabolites. The temperature stress which the wild cordyceps encounters during growth is responsible for the relatively high content of trehalose. These findings indicate that the immunity of the host insect and growth environment have a strong impact on the metabolomic variation in Isaria cicadae. The variation in metabolites suggests differential utilization value for the insect portions, coremia and mycelia of the fungus.

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Section: Resultsmentioning

confidence: 97%

Metabolomic variation in wild and cultured cordyceps and mycelia of Isaria cicadae

Zhang

Fan

et al. 2019

Biomedical Chromatography

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“…; Nieto‐Peñalver et al . ). In addition, other studies have showed that the endophytic diazotrophic bacterium G. diazotrophicus has an inhibitory potential against several other micro‐organisms of agricultural importance, such as Fusarium oxysporum (Logeshwarn et al .…”

Section: Discussionmentioning

confidence: 97%

“…The antagonistic capacity of G. diazotrophicus was previously evaluated by different authors demonstrating in vitro the ability of this bacterium to inhibit growth of the sugarcane phytopathogen X. albilineans (Piñ on et al 2002;Blanco et al 2005;Nieto-Peñalver et al 2014). In addition, other studies have showed that the endophytic diazotrophic bacterium G. diazotrophicus has an inhibitory potential against several other micro-organisms of agricultural importance, such as Fusarium oxysporum (Logeshwarn et al 2011), Xanthomonas campestris pv.…”

Section: Discussionmentioning

confidence: 99%

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Gluconacin fromGluconacetobacter diazotrophicusPAL5 is an active bacteriocin against phytopathogenic and beneficial sugarcane bacteria

et al. 2018

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“…El medio LGIM líquido, se empleó por Molinari & Bioardi (2013) para evaluar la producción de biomasa y levana, mientras que en el 2011, Dibut et al (2011) obtuvieron un biopreparado de uso agrícola en el medio SG. En las investigaciones realizadas para determinar la influencia del ácido glucónico en la actividad antagonista del microorganismo se usó el medio DYGS (Nieto-Peñalver et al, 2014), al igual que para investigar aspectos relacionados con el proceso de colonización endofítica (Alquéres et al, 2013). Aunque el medio de cultivo utilizado para el crecimiento del microorganismo puede ejercer efectos sobre su metabolismo, no se encontró en la literatura consultada ninguna investigación donde se demuestre su acción sobre el potencial como bacteria promotora del crecimiento vegetal de la especie bacteriana.…”

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Para el crecimiento de Gluconacetobacter diazotrophicus se han utilizado diferentes medios de cultivos, aunque existen pocos trabajos que ilustran cómo estos afectan las características del microorganismo y su efecto en la planta. El objetivo de esta investigación fue evaluar en condiciones in vitro e in vivo el efecto de cinco medios de cultivos sobre el potencial como promotor del crecimiento vegetal de esta especie bacteriana en zanahoria y remolacha. La concentración del microorganismo después de 48 horas de fermentación en zaranda rotatoria se mantuvo en el orden de 10 12 CFU. mL-1 para todos los medios de cultivo evaluados. Al finalizar el proceso fermentativo la bacteria presentó un crecimiento estable en un medio carente de nitrógeno combinado; sin embargo, las diferencias en los halos de solubilización en el medio NBRIP indican una afectación en la capacidad de solubilización de fósforo. El efecto de la aplicación de los productos finales de la fermentación sobre zanahoria y remolacha depende del medio utilizado para el crecimiento del microorganismo. Variantes como el SG y SYP producen una mayor influencia sobre el follaje, mientras que el medio MA (elaborado a base de arroz) sobresale por su efecto Article Info/Información del artículo

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Gluconic acid produced by Gluconacetobacter diazotrophicus Pal5 possesses antimicrobial properties

Cited by 26 publications

References 35 publications

Metabolomic variation in wild and cultured cordyceps and mycelia of Isaria cicadae

Metabolomic variation in wild and cultured cordyceps and mycelia of Isaria cicadae

Gluconacin fromGluconacetobacter diazotrophicusPAL5 is an active bacteriocin against phytopathogenic and beneficial sugarcane bacteria

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