Glucosamine, a naturally occurring amino monosaccharide, suppresses the ADP-mediated platelet activation in humans

Hua, Jian; Suguro, Shiori; Iwabuchi, Kazuhisa; Tsutsumi‐Ishii, Yuko; Sakamoto, Koji; Nagaoka, Isao

doi:10.1007/s00011-004-1312-y

Cited by 32 publications

(32 citation statements)

References 34 publications

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“…5 A and B). Usually, 1.5 g/day of glucosamine sulfate or hydrochloride (approximately 25 mg/kg) is administered to humans for treatment of osteoarthritis [22 -24], and serum glucosamine level reaches 0.020 ± 0.006 mM, as measured by a high performance liquid chromatography method using phenylisothiocyanate-derivatized glucosamine [25]. Moreover, in case of pharmacokinetic study a single dose of 6 g glucosamine has been administered to human volunteers [26].…”

Section: Discussionmentioning

confidence: 99%

Preventive actions of a high dose of glucosamine on adjuvant arthritis in rats

Hua

Suguro

Hirano³

et al. 2005

Inflamm. res.

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Section: Discussionmentioning

confidence: 99%

Preventive actions of a high dose of glucosamine on adjuvant arthritis in rats

Hua

Suguro

Hirano³

et al. 2005

Inflamm. res.

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“…In addition, extracellularly released ATP was quantified by the sensitive firefly luciferase assay, as described previously (35). In brief, 10 l aliquots of culture supernatants from LL-37-treated neutrophils (0.01ϳ5 g/ml; 37°C, 10 min and 18 h) or standard ATP were added to 0.1 ml of reaction buffer (50 mM Tris-acetate, 2 mM EDTA, 60 mM DTT, 0.072% BSA, and 10 mM magnesium acetate (pH 7.7)) containing 1 g/ml luciferase (Roche Molecular Biochemicals) and 0.1 mM D-luciferin (Wako Pure Chemical).…”

Section: Assay For the Effect Of Apyrase And Quantification Of Atp Inmentioning

confidence: 99%

An Antimicrobial Cathelicidin Peptide, Human CAP18/LL-37, Suppresses Neutrophil Apoptosis via the Activation of Formyl-Peptide Receptor-Like 1 and P2X7

Nagaoka

Tamura

Hirata

2006

The Journal of Immunology

241

202

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Peptide antibiotics possess the potent antimicrobial activities against invading microorganisms and contribute to the innate host defense. An antibacterial cathelicidin, human cationic antibacterial protein of 18 kDa/LL-37, not only exhibits potent bactericidal activities against Gram-negative and Gram-positive bacteria, but also functions as a chemoattractant for immune cells, including neutrophils. During bacterial infections, the life span of neutrophils is regulated by various pathogen- and host-derived substances. In this study, to further evaluate the role of LL-37 in innate immunity, we investigated the action of LL-37 on neutrophil apoptosis. Neutrophil apoptosis was assessed using human blood neutrophils based on the morphological changes. Of note, LL-37 dose dependently (0.01–5 μg/ml) suppressed neutrophil apoptosis, accompanied with the phosphorylation of ERK-1/2, expression of Bcl-xL (an antiapoptotic protein), and inhibition of caspase 3 activity. Interestingly, LL-37-induced suppression of neutrophil apoptosis was attenuated by the antagonists for formyl-peptide receptor-like 1 (FPRL1) and P2X7 nucleotide receptor. Of importance, the agonists for FPRL1 and P2X7 apparently suppressed neutrophil apoptosis. Collectively, these observations indicate that LL-37 cannot only kill bacteria, but also modulate (suppress) neutrophil apoptosis via the activation of FPRL1 and P2X7 in bacterial infections. Suppression of neutrophil apoptosis results in the prolongation of their life span, and may be advantageous for host defense against bacterial invasion.

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“…3) Glucosamine inhibited smooth muscle cell proliferation and reduced the atherosclerotic lesion in the aortic root of ApoE-null mice. 4) Moreover, a recent cohort study provides the evidence that glucosamine decreases total mortality.…”

Section: Discussionmentioning

confidence: 98%

“…1) Glucosamine has been also shown to have antiinflammatory and anti-atherosclerotic properties in experimental studies. [2][3][4] Recently, the vitamin and lifestyle cohort study has demonstrated that the use of glucosamine was significantly associated with decreased total mortality. 5) Taken together, glucosamine might decrease total mortality through controlling the atherosclerotic process.…”

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Oral Administration of Glucosamine Improves Vascular Endothelial Function by Modulating Intracellular Redox State

et al. 2017

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SummaryGlucosamine, used to treat osteoarthritis, has been shown to have anti-inflammatory and antiatherosclerotic effects in experimental studies. A recent cohort study has demonstrated that the use of glucosamine was significantly associated with decreased total mortality. Vascular endothelial function is a potent surrogate marker of atherosclerosis and cardiovascular mortality where oxidative stress could participate. Therefore, we investigated whether glucosamine improves vascular endothelial function and intracellular redox state. We examined the effects of oral glucosamine administration (3000 mg/day) for 4 weeks on flow-mediated vasodilation (FMD) and intraerythrocyte glutathione parameters in 20 volunteers. Nineteen age-matched volunteers served as controls. Glucosamine administration significantly increased FMD (from 7.0 ± 2.3 to 8.7 ± 2.3%, P = 0.022). In the control group, FMD did not change. Glucosamine administration significantly increased intraerythrocyte total glutathione levels (from 212.9 ± 46.2 to 240.6 ± 49.4 μmol/L, P = 0.006), intraerythrocyte reduced form of glutathione (GSH) levels (from 124.7 ± 42.6 to 155.2 ± 47.7 μmol/L; P = 0.004) and intraerythrocyte GSH/oxidized form of glutathione (GSSG) ratios (from 3.18 ± 1.64 to 3.88 ± 1.61, P = 0.04). In the control group, any glutathione parameters did not change. Moreover, a stepwise multivariate analysis revealed percent change of GSH/GSSG is the only independent predictor for those of FMD (standardized β = 0.58, P = 0.007) in the glucosamine group. Glucosamine administration improved FMD in association with amelioration of intraerythrocyte GSH/GSSG ratios. These results suggest that oral glucosamine administration might improve vascular endothelial function by modulating intracellular redox state. (Int Heart J 2017; 58: 926-932)

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Glucosamine, a naturally occurring amino monosaccharide, suppresses the ADP-mediated platelet activation in humans

Cited by 32 publications

References 34 publications

Preventive actions of a high dose of glucosamine on adjuvant arthritis in rats

Preventive actions of a high dose of glucosamine on adjuvant arthritis in rats

An Antimicrobial Cathelicidin Peptide, Human CAP18/LL-37, Suppresses Neutrophil Apoptosis via the Activation of Formyl-Peptide Receptor-Like 1 and P2X7

Oral Administration of Glucosamine Improves Vascular Endothelial Function by Modulating Intracellular Redox State

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