Glucosamine-conjugated nanoseeds for chemo-magneto hyperthermia therapy of cancer

Patharkar, Abhimanyu; Raval, Nidhi; Kalyane, Dnyaneshwar; Tambe, Vishakha; Anup, Neelima; More, Namdev; Kapusetti, Govinda; Kalia, Kiran; Tekade, Rakesh K.

doi:10.1016/j.jddst.2020.102295

Cited by 9 publications

(6 citation statements)

References 36 publications

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“…Conformational changes of pHILP due to the lower pH values of the tumour microenvironment facilitated the accumulation of SPIONs into tumour cells; hence, a tumour-selective imaging could be achieved. 67 The surface of SPIONs can be modified with other cellspecific molecules such as antibodies, 30 vitamins, 68 monosacharides, 69 and small molecules 70 in a similar fashion. Some cell-based approaches have also been developed for the delivery of SPIONs.…”

Section: Reviewmentioning

confidence: 99%

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Superparamagnetic iron oxide nanoparticles for magnetic hyperthermia: recent advancements, molecular effects, and future directions in the omics era

et al. 2022

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Section: Reviewmentioning

confidence: 99%

“…The surface of SPIONs can be modified with other cell-specific molecules such as antibodies, 30 vitamins, 68 monosacharides, 69 and small molecules 70 in a similar fashion. Some cell-based approaches have also been developed for the delivery of SPIONs.…”

Section: Recent Delivery and Targeting Technologies For Spionsmentioning

confidence: 99%

Superparamagnetic iron oxide nanoparticles for magnetic hyperthermia: recent advancements, molecular effects, and future directions in the omics era

et al. 2022

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“…After 24 h, cells were treated with anti-miR-FAM (20 pmol/well) and anti-miR-FAM- d CHA (20 pmol/well). After 8 h, the cellular internalization was observed with a confocal laser scanning microscope (Leica Microsystems, Wetzlar, Germany), and fluorescence intensity was analyzed by ImageJ . Similarly, the receptor blockade assay was performed except before treatment with anti-miR-FAM (20 pmol/well) and anti-miR-FAM- d CHA (20 pmol/well), and CD44 receptors of MDA-MB-231 cells were blocked by treatment with 5 mg/mL HA solution for 3 h.…”

Section: Methodsmentioning

confidence: 99%

Dendronized Polymeric Biomaterial for Loading, Stabilization, and Targeted Cytosolic Delivery of microRNA in Cancer Cells

Tambe

Patel

Shard

et al. 2022

ACS Appl. Bio Mater.

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MicroRNAs (miRNAs) are small non-coding RNAs involved in the fine-tuning of gene regulation. Anti-miRNA is a promising RNA-interference (RNAi) agent that potently regulates post-transcriptional expression of an abnormal gene by inhibiting its target mRNAs. To date, ONPATTRO and Leqvio are the only firstin-class USFDA-approved RNAi-therapeutics available in clinic. The deficiency of a fitting delivery carrier remains a primary hindrance to their clinical translation. To address this issue, this investigation reports the development of a dendronized polymeric nanobiomaterial involving a USFDA-approved biopolymer (hyaluronic acid, also called hyaluronan; HA) for the selective delivery of anti-miRNA into the cytosolic compartment of cancer cells. Dendrons are synthesized for focal cationization of HA to produce a cationic dendronized HA polymer (dCHA) using a ligated dendron motif approach. The synthesized dCHA is inert toward blood cells, as observed in the hemolysis assay. It also depicts a strong binding affinity for the CD44-receptor protein and is found to be neutral toward macrophages and albumin proteins (human origin; molecular simulation and docking tool: GROMACS). The developed approach is simple in application, offers high anti-miR21 loading, and avoids RNase enzymatic degradation of loaded anti-miRNA. The dCHA could efficiently escape the lysoendosomal compartment to mediate cytosolic delivery of the loaded anti-miRNA, ascribed to the proton sponge effect offered by weak basic groups of ligated dendron motifs in the dCHA architecture. The dCHA-loaded anti-miR21 upregulates the mRNA levels of Bax and CASP3 and downregulates the levels of Bcl2, accompanied by significant miR21 gene downregulation. Furthermore, under the influence of CD44-receptor blockade, a reduction in the cellular uptake of FAM-labeled anti-miR21 is observed compared to the control, inferring receptor-mediated uptake of dCHA. The conclusive outcome of this research advocates the use of dCHA to be a fit-to-purpose modality to load, preserve, and selectively deliver anti-miRNA-therapeutics to the cytosolic compartment of cancer cells. The developed approach has been tested using anti-miR21 as a model RNAi-therapeutic; however, the knowledge developed in this fundamental research can also be extended to other gene therapeutics, including DNA, siRNA, miRNA mimics, plasmid oligonucleotides, and so forth.

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“…Glucose and GlcN are delivered to cells via various glucose transporters (GLUTs) and sodium glucose transporters (SGLTs) expressed on cell membranes [9,16], many of which are overexpressed on tumor cells to facilitate glucose and GlcN delivery [17]. Owing to the aforementioned higher GlcN demand by tumor cells than by normal cells (GlcN effects) and the -NH 2 group of GlcN, which allows easy conjugation with the -COOH group of functional agents via an amide bond (-OH group of glucose is not easy for conjugation), GlcN has been widely applied as a conjugation ligand to increase delivery amounts of antitumor drugs [18][19][20][21], tumor imaging agents [21][22][23], and tumor therapeutic agents [24]. GlcN had been conjugated with various materials for drug delivery to tumor, tumor imaging, and tumor therapy.…”

Section: Introductionmentioning

confidence: 99%

“…GlcN had been conjugated with various materials for drug delivery to tumor, tumor imaging, and tumor therapy. These included poly(amidoamine) dendrimers to deliver camptothecin antitumor drug to human lung tumor (A549) cells in vitro and in vivo [18], graphene quantum dots to deliver curcumin antitumor drug to human breast tumor (MCF-7) cells in vitro [19], niosomal formulation to deliver doxorubicin antitumor drug to skin melanoma tumor (B6F10) cells in vitro [20], InP/ZnS quantum dots to deliver doxorubicin to human lung epithelial tumor (A549) cells and human ovarian tumor (OVCAR-3) cells in vitro and to image tumor cells [21], near infrared fluorescent probes for breast [22] and prostate [23] tumor imaging in vitro and in vivo, and multifunctional doxorubicin loaded gadolinium/cobalt@iron oxide-dendrimer-nanoseeds for chemo-magneto hyperthermia treatment of human prostate tumor (PC3) cells in vitro [24].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Tumor Imaging Using Glucosamine-Conjugated Polyacrylic Acid-Coated Ultrasmall Gadolinium Oxide Nanoparticles in Magnetic Resonance Imaging

Liu

Yue

et al. 2022

IJMS

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Owing to a higher demand for glucosamine (GlcN) in metabolic processes in tumor cells than in normal cells (i.e., GlcN effects), tumor imaging in magnetic resonance imaging (MRI) can be highly improved using GlcN-conjugated MRI contrast agents. Here, GlcN was conjugated with polyacrylic acid (PAA)-coated ultrasmall gadolinium oxide nanoparticles (UGONs) (davg = 1.76 nm). Higher positive (brighter or T1) contrast enhancements at various organs including tumor site were observed in human brain glioma (U87MG) tumor-bearing mice after the intravenous injection of GlcN-PAA-UGONs into their tail veins, compared with those obtained with PAA-UGONs as control, which were rapidly excreted through the bladder. Importantly, the contrast enhancements of the GlcN-PAA-UGONs with respect to those of the PAA-UGONs were the highest in the tumor site owing to GlcN effects. These results demonstrated that GlcN-PAA-UGONs can serve as excellent T1 MRI contrast agents in tumor imaging via GlcN effects.

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Glucosamine-conjugated nanoseeds for chemo-magneto hyperthermia therapy of cancer

Cited by 9 publications

References 36 publications

Superparamagnetic iron oxide nanoparticles for magnetic hyperthermia: recent advancements, molecular effects, and future directions in the omics era

Superparamagnetic iron oxide nanoparticles for magnetic hyperthermia: recent advancements, molecular effects, and future directions in the omics era

Dendronized Polymeric Biomaterial for Loading, Stabilization, and Targeted Cytosolic Delivery of microRNA in Cancer Cells

Enhanced Tumor Imaging Using Glucosamine-Conjugated Polyacrylic Acid-Coated Ultrasmall Gadolinium Oxide Nanoparticles in Magnetic Resonance Imaging

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