Glucosamine modulates propranolol pharmacokinetics via intestinal permeability in rats

Shaker, Hanadi A. Al; Qinna, Nidal A.; Badr, Mujtaba M; Omari, Mahmoud M. H. Al; Idkaidek, Nasir; Matalka, Khalid Z.; Badwan, Adnan A.

doi:10.1016/j.ejps.2017.05.012

Cited by 12 publications

(9 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All perfusion buffers were freshly prepared according to Al Shaker et al (2017) using sterile technique and kept warm in a water bath at 37 °C prior use. Perfusion buffer I was prepared by adding 0.9 mM MgCl 2, 0.5 mM EDTA, and 0.5 mM Tris base, to 0.5 L Hank’s Balanced Salt Solution (HBSS, without Ca 2+ and Mg 2+ ) [20]. Perfusion buffer II was prepared by adding 0.5 mM Tris base to 0.5 L HBSS (with Ca 2+ and Mg 2+ ).…”

Section: Methodsmentioning

confidence: 99%

Hepatoprotective Actions of Ascorbic Acid, Alpha Lipoic Acid and Silymarin or Their Combination Against Acetaminophen-Induced Hepatotoxicity in Rats

et al. 2019

Self Cite

View full text Add to dashboard Cite

Background and objectives: Ascorbic acid, alpha lipoic acid (ALA) and silymarin are well-known antioxidants that have hepatoprotective effects. This study aims to investigate the effects of these three compounds combined with attenuating drug-induced oxidative stress and cellular damage, taking acetaminophen (APAP)-induced toxicity in rats as a model both in vivo and in vitro. Materials and Methods: Freshly cultured primary rat hepatocytes were treated with ascorbic acid, ALA, silymarin and their combination, both with and without the addition of APAP to evaluate their in vitro impact on cell proliferation and mitochondrial activity. In vivo study was performed on rats supplemented with the test compounds or their combination for one week followed by two toxic doses of APAP. Results: Selected liver function tests and oxidative stress markers including superoxide dismutase (SOD), malondialdehyde (MDA) and oxidized glutathione (GSSG) were detected. The in vivo results showed that all three pretreatment compounds and their combination prevented elevation of SOD and GSSG serum levels indicating a diminished burden of oxidative stress. Moreover, ascorbic acid, ALA and silymarin in combination reduced serum levels of liver enzymes; however, silymarin markedly maintained levels of all parameters to normal ranges. Silymarin either alone or combined with ascorbic acid and ALA protected cultured rat hepatocytes and increased cellular metabolic activity. The subjected agents were capable of significantly inhibiting the presence of oxidative stress induced by APAP toxicity and the best result for protection was seen with the use of silymarin. Conclusions: The measured liver function tests may suggest an augmented hepatoprotection of the combination preparation than when compared individually.

show abstract

Section: Methodsmentioning

confidence: 99%

Hepatoprotective Actions of Ascorbic Acid, Alpha Lipoic Acid and Silymarin or Their Combination Against Acetaminophen-Induced Hepatotoxicity in Rats

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Liver perfusion buffers were freshly prepared under sterile techniques (Al Shaker et al 25 ). Perfusion buffer I was prepared by adding 0.9 mM MgCl 2 (95.211 g/mol), 0.5 mM EDTA (292.24 g/mol), and 0.5 mM Tris base (121.14 g/mol) to 0.5 L HBSS (without Ca 2+ and Mg 2+ ).…”

Section: Methodsmentioning

confidence: 99%

“…The adult rat was placed on a surgical board (Plas Labs, Lansing, MI, USA), above a heating pad maintained at 37°C, and anesthetized by isoflurane (5% for induction and 2.5% for maintenance). 25 A midline longitudinal incision was made to expose the rat’s viscera. Hepatic portal vein was sighted and cannulated with an 18-gauge angiocath (Becton Dickinson, Mountain View, CA, USA) and perfused with a prewarmed (37°C) perfusion buffer I at a flow rate of 10 mL/min.…”

Section: Methodsmentioning

confidence: 99%

Antioxidative stress effects of vitamins C, E, and B<sub>12</sub>, and their combination can protect the liver against acetaminophen-induced hepatotoxicity in rats

Abdulkhaleq¹,

Alhussainy²,

Badr³

et al. 2018

DDDT

Self Cite

View full text Add to dashboard Cite

BackgroundSeveral vitamins, including C, E, and B12, have been recognized as antioxidants and have shown hepatoprotective effects against the hepatotoxicity caused by acetaminophen (APAP) overdose. The current investigation aims to study the effect of these vitamins and their combination in protecting the liver from APAP hepatotoxicity in rats.Materials and methodsAn in vitro model of freshly isolated rat hepatocytes was utilized for assessing hepatocyte mitochondrial activity conducted by cell proliferation assay (MTT). The isolated hepatocytes were treated with vitamin C, vitamin E, vitamin B12 and their combination, with and without further addition of toxic concentrations of APAP. In addition, an in vivo experiment was carried out on Sprague Dawley rats treated intraperitoneally for 8 days with emulsions of the vitamins or their combination prior to injecting them with APAP.ResultsIn vitro results showed that vitamins C and B and the combination preparation significantly increased the percentage of hepatocyte mitochondrial activity, both with and without the addition of APAP (P<0.01). The mitochondrial activity in the isolated cultured hepatocytes was further enhanced with APAP addition. In vivo, the vitamins and their combination effectively reduced APAP-induced serum liver enzymes levels, namely ALT, AST, and ALP, and also attenuated oxidative stress and lipids peroxidation confirmed by the results of glutathione, superoxide dismutase, and maloondialdehyde.ConclusionPretreatment with vitamins C, E, B12, or their combination was found to be beneficial in preventing in vivo hepatic oxidative stress induced by APAP overdose. Vitamin C on its own showed superior protection against APAP-induced liver injury in rats compared to the other vitamins. The proliferation of APAP-intoxicated liver cells in vitro was highest when protected with the vitamins’ combination.

show abstract

“…The liver of anaesthetized rat with inhaled isoflurane were perfused with collagenase enzyme as described by [30]. Liver cells were dispersed gently in a sterile petri dish, and prepared for hepatocyte culture as described elsewhere [31].…”

Section: Methodsmentioning

confidence: 99%

Critical pharmacokinetic and pharmacodynamic drug-herb interactions in rats between warfarin and pomegranate peel or guava leaves extracts

Alnaqeeb

Mansor

Mallah

et al. 2019

BMC Complement Altern Med

Self Cite

View full text Add to dashboard Cite

BackgroundIn-depth information of potential drug-herb interactions between warfarin and herbal compounds with suspected anticoagulant blood thinning effects is needed to raise caution of concomitant administration. The current study aimed to investigate the impact of co-administration of pomegranate peel and guava leaves extracts, including their quality markers namely; ellagic acid and quercetin, respectively, on warfarin’s in vivo dynamic activity and pharmacokinetic actions, in addition to potential in vitro cytochrome P450 enzymes (CYP) inhibition.MethodsInfluence of mentioned extracts and their key constituents on warfarin pharmacodynamic and kinetic actions and CYP activity were evaluated. The pharmacodynamic interactions were studied in Sprague Dawley rats through prothrombin time (PT) and International Normalized Ratio (INR) measurements, while pharmacokinetic interactions were detected in vivo using a validated HPLC method. Furthermore, potential involvement in CYP inhibition was also investigated in vitro on isolated primary rat hepatocytes.ResultsPreparations of pomegranate peel guava leaf extract, ellagic acid and quercetin in combination with warfarin were found to exert further significant increase on PT and INR values (p < 0.01) than when used alone (p < 0.05). Pomegranate peel extract showed insignificant effects on warfarin pharmacokinetics (p > 0.05), however, its constituent, namely, ellagic acid significantly increased warfarin Cmax (p < 0.05). Guava leaves extract and quercetin resulted in significant increase in warfarin Cmax when compared to control (p < 0.01). Furthermore, guava leaves extract showed a significant effect on changing the AUC, CL and Vz. Significant reduction in CYP2C8, 2C9, and 3A4 was seen upon concomitant use of warfarin with ellagic acid, guava leaves and quercetin, unlike pomegranate that insignificantly affected CYP activities.ConclusionAll combinations enhanced the anticoagulant activity of warfarin as the results of in vivo and in vitro studies were consistent. The current investigation confirmed serious drug herb interactions between warfarin and pomegranate peel or guava leaf extracts. Such results might conclude a high risk of bleeding from the co-administration of the investigated herbal drugs with warfarin therapy. In addition, the results raise attention to the blood-thinning effects of pomegranate peel and guava leaves when used alone.

show abstract

Glucosamine modulates propranolol pharmacokinetics via intestinal permeability in rats

Cited by 12 publications

References 41 publications

Hepatoprotective Actions of Ascorbic Acid, Alpha Lipoic Acid and Silymarin or Their Combination Against Acetaminophen-Induced Hepatotoxicity in Rats

Hepatoprotective Actions of Ascorbic Acid, Alpha Lipoic Acid and Silymarin or Their Combination Against Acetaminophen-Induced Hepatotoxicity in Rats

Antioxidative stress effects of vitamins C, E, and B<sub>12</sub>, and their combination can protect the liver against acetaminophen-induced hepatotoxicity in rats

Critical pharmacokinetic and pharmacodynamic drug-herb interactions in rats between warfarin and pomegranate peel or guava leaves extracts

Contact Info

Product

Resources

About