Glucose-6-phosphate dehydrogenase promotes the infection of Chilli veinal mottle virus through affecting ROS signaling in Nicotiana benthamiana

Yang, Daoyong; Peng, Qiao; Cheng, Yongchao; Xi, Dehui

doi:10.1007/s00425-022-04010-1

Cited by 12 publications

(11 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Electrolyte leakage and the content of MDA can reflect the degree of damage to plants caused by various stresses (Yang et al, 2022). At 8 dpi, the electrolyte leakage in HCPro‐OX plants was significantly higher than that in WT plants under TMV or CMV infection (Figure 2c).…”

Section: Resultsmentioning

confidence: 99%

“…The above results indicate that HCPro‐OX plants suffered more severe damage during the early stage and less damage at the later stage of infection. The expression of the respiratory burst oxidase homologous D gene ( Rboh‐D ) can reflect ROS burst levels in plants well (Yang et al, 2022). Thus, the relative expression of Rboh‐D was determined by RT‐qPCR (Figure 2e).…”

Section: Resultsmentioning

confidence: 99%

“…In this study, the tolerance of HCPro-OX plants to different heterologous viruses at different periods of infection was also related to SA. on ChiVMV-GFP infection was related to ROS production regulated by respiratory burst oxidase homologue B (RBOHB) (Yang et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

“…Studies have shown that to resist cell death caused by excessive accumulation of ROS, plants have evolved antioxidant defence mechanisms, which involve POD, SOD, APX and CAT (Yang et al, 2022), so we measured the activity of these enzymes to explore whether the response of HCPro-OX plants to heterologous viruses occurred through an antioxidant mechanism. After virus inoculation, the activities of CAT, APX and POD were generally lower in HCPro-OX plants than WT plants in the early stage but higher than WT plants in the later stage except that of SOD (Figure 3a-d).…”

Section: Changes In Antioxidant System Of Hcpro-ox Plantsmentioning

confidence: 99%

See 3 more Smart Citations

HCPro affects heterologous virus infection through salicylic acid and auxin pathways

et al. 2023

Self Cite

View full text Add to dashboard Cite

Mixed infection by plant viruses is common in nature, but how a key viral protein of one virus affects the infection by heterologous viruses is not yet fully understood. The helper component proteinase (HCPro) is a widely studied RNA silencing suppressor encoded by viruses of the family Potyviridae. Here, we investigated the defence response of Nicotiana tabacum plants overexpressing HCPro of chilli veinal mottle virus (HCPro‐OX) to tobacco mosaic virus and cucumber mosaic virus infection. We monitored the physiological and molecular changes of HCPro‐OX plants in response to virus infection. The results showed that HCPro‐OX plants under virus infection exhibited higher susceptibility at the early stage but stronger tolerance at the later stage compared to wild‐type plants. The tolerance to heterologous virus infection of HCPro‐OX plants corresponded to a lower level of reactive oxygen species accumulation and higher activities of several antioxidant enzymes. Reverse transcription‐quantitative PCR assays showed that the expression of genes related to salicylic acid (SA) pathways was significantly upregulated, but the expression of genes related to the auxin pathways was downregulated at the late stage of virus infection in HCPro‐OX plants compared to wild‐type plants. By contrast, the situation in the early stage of virus infection was reversed. In addition, pretreatment with SA, the auxin naphthylacetic acid (NAA) and their respective inhibitors 1‐aminobenzotriazole (ABT) and naphthalam (NPA) further confirmed the antagonistic effects of SA and NAA in the response of HCPro‐OX plants to heterologous virus infection. Thus, our results demonstrate that HCPro affects heterologous virus infection through SA and auxin pathways.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Changes In Antioxidant System Of Hcpro-ox Plantsmentioning

confidence: 99%

See 2 more Smart Citations

HCPro affects heterologous virus infection through salicylic acid and auxin pathways

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…G6PDHs have been identified as important regulators of many plant species’ growth and abiotic stress responses ( Wakao and Benning, 2005 ; Landi et al., 2021 ; Tian et al., 2021 ). While G6PDHs have been cloned successfully from a range of plants, including soybean ( Zhao et al., 2020 ), tobacco ( Yang et al., 2022 ), tomato ( Landi et al., 2016 ), barley ( Cardi et al., 2013 ), wheat ( Nemoto and Sasakuma, 2000 ), and Arabidopsis ( Wakao and Benning, 2005 ), little is known regarding this gene family in maize. Five maize G6PDH family members were identified ( ZmG6PDH1-5 ; Table 1 ).…”

Section: Discussionmentioning

confidence: 99%

ZmG6PDH1 in glucose-6-phosphate dehydrogenase family enhances cold stress tolerance in maize

Cai²,

Yu³

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

Glucose-6-phosphate dehydrogenase (G6PDH) is a key enzyme in the pentose phosphate pathway responsible for the generation of nicotinamide adenine dinucleotide phosphate (NADPH), thereby playing a central role in facilitating cellular responses to stress and maintaining redox homeostasis. This study aimed to characterize five G6PDH gene family members in maize. The classification of these ZmG6PDHs into plastidic and cytosolic isoforms was enabled by phylogenetic and transit peptide predictive analyses and confirmed by subcellular localization imaging analyses using maize mesophyll protoplasts. These ZmG6PDH genes exhibited distinctive expression patterns across tissues and developmental stages. Exposure to stressors, including cold, osmotic stress, salinity, and alkaline conditions, also significantly affected the expression and activity of the ZmG6PDHs, with particularly high expression of a cytosolic isoform (ZmG6PDH1) in response to cold stress and closely correlated with G6PDH enzymatic activity, suggesting that it may play a central role in shaping responses to cold conditions. CRISPR/Cas9-mediated knockout of ZmG6PDH1 on the B73 background led to enhanced cold stress sensitivity. Significant changes in the redox status of the NADPH, ascorbic acid (ASA), and glutathione (GSH) pools were observed after exposure of the zmg6pdh1 mutants to cold stress, with this disrupted redox balance contributing to increased production of reactive oxygen species and resultant cellular damage and death. Overall, these results highlight the importance of cytosolic ZmG6PDH1 in supporting maize resistance to cold stress, at least in part by producing NADPH that can be used by the ASA-GSH cycle to mitigate cold-induced oxidative damage.

show abstract

The role of reactive oxygen species in plant-virus interactions

Xu,

Zhang,

Zhang

et al. 2024

Plant Cell Rep

View full text Add to dashboard Cite

Glucose-6-phosphate dehydrogenase promotes the infection of Chilli veinal mottle virus through affecting ROS signaling in Nicotiana benthamiana

Cited by 12 publications

References 51 publications

HCPro affects heterologous virus infection through salicylic acid and auxin pathways

HCPro affects heterologous virus infection through salicylic acid and auxin pathways

ZmG6PDH1 in glucose-6-phosphate dehydrogenase family enhances cold stress tolerance in maize

The role of reactive oxygen species in plant-virus interactions

Contact Info

Product

Resources

About