Glucose biosensors based on oxygen electrode with sandwich-type membranes

Abstract

A glucose biosensor based on an amperometric oxygen electrode has been developed. Polycarbonate and Silastic membranes were assembled (glued together) to form a multilayer sandwich glucose diffusion barrier. The effects of the glue layer composition and thickness of the Silastic membrane on sensor response parameters have been investigated in order to optimize the sensor. The parameters measured were the sensitivity, the concentration range of the linear dependence of the sensor response to glucose, and the long-term operation time. The sensors with the sandwich-type glucose diffusion membrane (Silastic membrane prepared from 20% Silastic suspension, glue layer prepared from polyurethane, 0.5 w/v % in ThF solution and standard polycarbonate membrane) demonstrated linearity of response up to 520 mg/dl glucose at 25 degrees C and up to 400 mg/dl at 37 degrees C. These sensor showed good reproducibility of response without significant interference effects (from 1 to 5% of the background current value). The long-term continuous operational time of the sensors was over 40 days at 37 degrees C, and over 60 days at 25 degrees C.

Continuous amperometric monitoring of subcutaneous oxygen in rabbit by telemetry

“…Measurements of tissue oxygen may help to understand this issue, as studied by Ertefai and Gough [12] and by Fischer [18]. In addition, an oxygen sensor may be modi ed to measure glucose, a technique successfully used by the San Diego group [13,15,16] and by Yang et al [39]. In this method, two measurements are made simultaneously: oxygen without use of an enzyme, and oxygen in the presence of glucose oxidase.…”

Implantable Electrochemical Sensors for Biomedical and Clinical Applications: Progress, Problems, and Future Possibilities.

“…1 can be oxidized with the resultant current proportional to the glucose concentration [29][30][31]. 1 can be oxidized with the resultant current proportional to the glucose concentration [29][30][31].…”

Glucose Microbiosensor Based on MnO2 and Glucose Oxidase Modified Carbon Fiber Microelectrode

“…Among these devices, glucose biosensors have remained the most attractive, mainly due to the significance of strict diabetes mellitus control, which enhances their potential commercialization [6]. In various, particularly biomedical applications, such as continuous in vivo glucose monitoring with implantable or needle-like sensors [5,7], measurements in microenvironments, or detection in resistive organic media, their miniaturization down to micro-and submicrometer design is dictated.…”