Glucose-containing peritoneal dialysis fluids regulate leptin secretion from 3T3-L1 adipocytes

Abstract

These results suggest that the PD-induced hyperleptinaemia could, in part, be mediated by the effect of glucose-based dialysis fluids on leptin production by adipocytes via activation of the hexosamine biosynthetic pathway.

A cute plasma ghrelin and leptin responses to oral feeding or intraperitoneal hypertonic glucose-based dialysate in patients with chronic renal failure

“…The latter finding may be secondary to a combination of peritoneal clearance of leptin during the PD exchange test [24,57,58] and/or of circadian changes in leptinemia [59]. Intraperitoneal glucose has been demonstrated to exert a direct stimulatory effect of leptin secretion by adipocytes, but this effect may not be apparent before 24 to 48 hours after stimulation [60].…”

Inflammation in Peritoneal Dialysis

“…Leptin activates immune system and serves as a mediator of inflammation [65]. Glucose-based PDF induces a higher leptin secretion by a murine adipocyte cell line 3T3-L1 compared to dialysate with physiological glucose concentration via the hexosamine pathway [66]. We have demonstrated that the full-length isoform of leptin receptor, Ob-Rb, is expressed in HPMC and its expression is up-regulated following exposure to glucose [67].…”

Membrane Biology During Peritoneal Dialysis

“…Whether such paracrine interaction is operating between peritoneal adipocytes and MCs remains to be explored. To the best of our knowledge, there is only one previous study on the effect of PDF on adipocytes that demonstrates increased leptin synthesis in a murine adipocyte cell line (3T3-L1) by glucosecontaining PDFs [29]. It is likely that proinflammatory mediators released by MCs upon exposure to PDF could induce functional alteration of adjacent adipocytes.…”