Glucose-free fructose production from Jerusalem artichoke using a recombinant inulinase-secreting Saccharomyces cerevisiae strain

Yu, Jing; Jiang, Jiaxi; Ji, Wangming; Li, Yuyang; Liu, Jianping

doi:10.1007/s10529-010-0414-6

Cited by 17 publications

(7 citation statements)

References 15 publications

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“…The resulting hydrolysate contained 78.2% of fructose. Yu et al [82] developed a novel recombinant inulinase-secreting Saccharomyces cerevisiae strain to produce glucose-free fructose from Jerusalem artichoke. Such a recombinant biocatalyst was capable of hydrolyzing inulin into fructose and glucose, and subsequently metabolizing glucose.…”

Section: Bioproducts Derived From Jerusalem Artichokementioning

confidence: 99%

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The prospects of Jerusalem artichoke in functional food ingredients and bioenergy production

Yang

Corscadden

et al. 2015

Biotechnology Reports

138

108

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Jerusalem artichoke, a native plant to North America has recently been recognized as a promising biomass for bioeconomy development, with a number of advantages over conventional crops such as low input cultivation, high crop yield, wide adaptation to climatic and soil conditions and strong resistance to pests and plant diseases. A variety of bioproducts can be derived from Jerusalem artichoke, including inulin, fructose, natural fungicides, antioxidant and bioethanol. This paper provides an overview of the cultivation of Jerusalem artichoke, derivation of bioproducts and applicable production technologies, with an expectation to draw more attention on this valuable crop for its applications as biofuel, functional food and bioactive ingredient sources.

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Section: Bioproducts Derived From Jerusalem Artichokementioning

confidence: 99%

“…[77], [78], [79], [80], [81], [82], [83], [84], [85], [86], [87] Such-prepared fructose can be further converted into ethanol. For the ethanol production starting with tubers through SHF process, hydrolysis step has significant impacts on the following fermentation step.…”

Section: Bioproducts Derived From Jerusalem Artichokementioning

confidence: 99%

The prospects of Jerusalem artichoke in functional food ingredients and bioenergy production

Yang

Corscadden

et al. 2015

Biotechnology Reports

138

108

View full text Add to dashboard Cite

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“…6 in that report, the productivity should be less than 3 g/L/h33. Finally, another HXK null and inulinase expression S. cerevisiae Y16/pHC-PinuT strain produced 9.2% (w/v) glucose free fructose in 24 h with a productivity of 3.83 g/L/h and the yield was not provided29. In this study, K. marxianus YGR003 used 400 g/L JAT and produced 234.44 g/L fructose with a productivity of 8.37 g/L/h.…”

Section: Discussionmentioning

confidence: 91%

“…The preparation of glucose isomerase and large scale chromatography are relative high cost procedure. Inulin contains mainly fructose, it is excellent substrate for high fructose syrup production1725262728293031. However, inulinase is required in inulin hydrolysis.…”

Section: Discussionmentioning

confidence: 99%

“…Constructing a fructose assimilation deficient and inulin expressing yeast strain should enhance the fructose production from inulin or JAT. Fructose assimilation deficient strains were constructed in S. cerevisiae previously through which glucose-free fructose was produced with JAT2933. A 8.5% of fructose syrup was obtained through fermentation by S. cerevisiae ATCC 36859 with high fructose corn syrup and hydrolyzed JAT juice with a yield of near 100% and a productivity of 1.33 g/L/h34.…”

Section: Discussionmentioning

confidence: 99%

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Identification of hexose kinase genes in Kluyveromyces marxianus and thermo-tolerant one step producing glucose-free fructose strain construction

Zhang

Lü

Wang

et al. 2017

Sci Rep

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In yeast, the hexose assimilation is started at hexose phosphorylation. However, in Kluyveromyces marxianus, the hexokinase (HXK) and glucokinase (GLK) genes were not identified by experiments. Meanwhile, the glucose-free fructose product requires more cost-efficient method. In this study, the KmHXK1 and KmGLK1 genes were functionally identified through gene disruption, over-expression and recombinant enzymes characterization. Both glucose and fructose assimilation ability decreased significantly in KmHXK1 disrupted strain YLM001, however, this ability was not changed obviously in KmGLK1 disrupted strain YLM002. When over-expressing KmGLK1 in YLM001, only the glucose assimilation ability was recovered in obtained strain (YLM005). The kinetic constant analysis of recombinant enzymes also proved that KmHXK1 could phosphorylate glucose (Vmax 553.01 U/mg, Km 0.83 mM) and fructose (Vmax 609.82 U/mg, Km 0.52 mM), and KmGLK1 only phosphorylate glucose with a Vmax of 0.73 U/mg and a Km 4.09 mM. A thermo-tolerant strain YGR003 which produced glucose-free fructose from Jerusalem artichoke tuber in one step was constructed based on the obtained information. The highest production and fastest productivity were 234.44 g/L and 10.26 g/L/h, respectively, which were several folds of the results in previous reports.

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Engineering an industrial Saccharomyces cerevisiae strain with the inulinase gene for more efficient ethanol production from Jerusalem artichoke tubers

Yuan

Zhao

et al. 2013

Engineering in Life Sciences

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Engineering industrial microbial strains with inulinase production for developing a consolidated bioprocessing (CBP) strategy is a desirable solution to biofuel production from Jerusalem artichoke tubers. In this study, the integrative vector pFA6a‐rDNA‐PGK‐INU was generated by fusing the 3.3‐kb ribosomal DNA fragment with the phosphoglycerate kinase promoter to regulate the expression of the inulinase gene isolated from Kluyveromyces marxianus, which was then integrated into the chromosome of an industrial Saccharomyces cerevisiae strain for ethanol production from Jerusalem artichoke tubers. Compared to the host strain, no significant difference was observed in the growth of the recombinant yeast, but its inulinase production was enhanced: 22.9 versus 10.6 U/mL for aerobic seed cultures and 14.5 versus 10.0 U/mL for ethanol fermentation, which consequently facilitated the CBP process for ethanol production: 72.5 g/L ethanol produced in a fermentation time of 48 h, while only 67.0 g/L ethanol was produced by the host strain in a fermentation time of 60 h. Thus, the ethanol productivity was increased from 1.12 to 1.51 g/L/h, presenting an increase of 34.8%.

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Glucose-free fructose production from Jerusalem artichoke using a recombinant inulinase-secreting Saccharomyces cerevisiae strain

Cited by 17 publications

References 15 publications

The prospects of Jerusalem artichoke in functional food ingredients and bioenergy production

The prospects of Jerusalem artichoke in functional food ingredients and bioenergy production

Identification of hexose kinase genes in Kluyveromyces marxianus and thermo-tolerant one step producing glucose-free fructose strain construction

Engineering an industrial Saccharomyces cerevisiae strain with the inulinase gene for more efficient ethanol production from Jerusalem artichoke tubers

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