Glucose in platelet additive solutions: To add or not to add?

Gyöngyössy‐Issa, Maria I.C.

doi:10.1016/j.transci.2011.03.003

Cited by 15 publications

(9 citation statements)

References 203 publications

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“…MTR also presented strong correlation with the metabolic parameters analyzed. Our data confirm that the decrease in ΔΨm is related to the period of storage (22,23). However, the association of MTG and MTR provided information regarding the ΔΨm and about mitochondrial swelling, which granted more information concerning the organelles under study when compared with the classical marker JC-1.…”

Section: Resultssupporting

confidence: 79%

Comparison of JC‐1 and MitoTracker probes for mitochondrial viability assessment in stored canine platelet concentrates: A flow cytometry study

Marcondes¹,

Terra

Lasta

et al. 2018

Cytometry Pt A

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Mitochondria perform crucial roles in many biochemical processes, and mitochondrial depolarization is an early sign of platelet apoptosis. The mitochondrial membrane potential is usually evaluated through JC-1 probe, but it can also be assessed with MitoTracker probes. Our aim was to evaluate mitochondrial viability in stored canine platelet concentrates (PCs) with the fluorescent probes JC-1 and MitoTracker. Platelets from 22 canine PCs were stained with JC-1 and MitoTracker probes on days 1, 3, and 5 of storage. Data on metabolic parameters were also collected for correlation studies. Results of JC-1 and MitoTracker revealed a decrease in mitochondrial membrane potential in day 5 of storage compared to days 1 and 3, providing evidence of mitochondrial depolarization, a finding that was confirmed by the data on metabolic parameters. MitoTracker probes also added information regarding platelet swelling. In conclusion, MitoTracker probes offered a more complete mitochondrial analysis in the evaluation of stored canine PCs.

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Section: Resultssupporting

confidence: 79%

Comparison of JC‐1 and MitoTracker probes for mitochondrial viability assessment in stored canine platelet concentrates: A flow cytometry study

Marcondes¹,

Terra

Lasta

et al. 2018

Cytometry Pt A

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“…However, both glucose consumption and lactate production were accelerated compared to what is considered acceptable during liquid storage . In addition, the ratio of lactate produced as a consequence of glucose consumption was reduced, which has previously been shown to occur in the presence of high glucose concentrations . Despite this, the pH was maintained above 6.4, as recommended by the Council of Europe guidelines, in all groups.…”

Section: Discussionmentioning

confidence: 83%

“…30 In addition, the ratio of lactate produced as a consequence of glucose consumption was reduced, which has previously been shown to occur in the presence of high glucose concentrations. 31,32 Despite this, the pH was maintained above 6.4, as recommended by the Council of Europe guidelines, in all groups. However, the PLTs reconstituted in PAS-G had a significantly lower pH than the other two groups, due to the lower starting pH of the solution.…”

Section: Frozen Plts In Pas-gmentioning

confidence: 99%

PAS‐G supports platelet reconstitution after cryopreservation in the absence of plasma

et al. 2013

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“…In stored PLTs, glucose is provided through the plasma portion of the storage medium and sufficient glucose levels are maintained even in PLTs suspended in approximately 30–35% plasma/65–70% glucose‐free platelet additive solutions (PASs). While other substrates, such as acetate, also act as energy sources, data on the relative importance of these energy sources are mixed .…”

Section: Introductionmentioning

confidence: 97%

Storage of apheresis platelet concentrates after manual replacement of >95% of plasma with PAS 5

et al. 2014

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Glucose in platelet additive solutions: To add or not to add?

Cited by 15 publications

References 203 publications

Comparison of JC‐1 and MitoTracker probes for mitochondrial viability assessment in stored canine platelet concentrates: A flow cytometry study

Comparison of JC‐1 and MitoTracker probes for mitochondrial viability assessment in stored canine platelet concentrates: A flow cytometry study

PAS‐G supports platelet reconstitution after cryopreservation in the absence of plasma

Storage of apheresis platelet concentrates after manual replacement of >95% of plasma with PAS 5

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