Glucose-induced production of a Penicillium purpurogenum xylanase by Aspergillus nidulans

Abstract

The heterologous secretion of xylanase B from Penicillium purpurogenum using glucose as inducer was performed in Aspergillus nidulans. For this purpose, plasmid pEVXB, containing the xylanase B cDNA (including its own signal peptide) under the control of the glyceraldehyde- 3-phosphate dehydrogenase promoter, was constructed and used to transform A. nidulans. Analysis of transformed clones showed that several of them secreted extracellular xylanase activity when grown in a medium containing glucose. The clone showing the highest xylanase activity was chosen for further work. When this clone was grown on glucose, xylanase activity (0.72 U/ml), was detected in the culture supernatant. This was confirmed by a zymogram analysis and by the amplification of xynB cDNA from this clone. To our knowledge, this is the first example of the production of a xylanase from Penicillium in heterologous fungal hosts using glucose as inducer.This work was supported by grants from DICYT- USACH, FONDECYT 1100084 and UNAB DI-03-10/R. I. Vaca acknowledges support from the ‘‘Programa Bicentenario de Ciencia y Tecnologı´a’’ program (Chile), project PDA13

Identification and Functional Analysis of the Mycophenolic Acid Gene Cluster of Penicillium roqueforti

“…Total RNA was isolated exactly as described previously [ 16 ]. RT-PCR experiments were performed as described by Ravanal et al [ 17 ]. RACE-PCR experiments were performed with the FirstChoice RLM-RACE Kit (Invitrogen, USA) according to the manufacturer`s protocol.…”

The pcz1 Gene, which Encodes a Zn(II)2Cys6 Protein, Is Involved in the Control of Growth, Conidiation, and Conidial Germination in the Filamentous Fungus Penicillium roqueforti

“…RT-PCR experiments were performed essentially as described by Ravanal et al [ 18 ]. PCR conditions were the same as described by Diaz et al [ 19 ].…”

Microbial xylanases and their biomedical applications: a review

“…80 Agro waste substrates like corncob, rice straw, wheat straw, wheat bran, corn stalk and bagasse can be used as a substrate for xylanase production by certain microorganisms like Aspergillus awanian and Penicilluim purpurogenum (Ravanal et al, 2012). 74 Cellulase free Xylanase producer has been reported in Bacillus sp and fungi (Dey et al, 1992). 25 Xylanase activity is found to be higher in fungal system (with maximum activity of 3350 IU/ml In Trichoderma reesei) than Bacterial systems (Haapala et al 1994).…”