Glucose inhibits haemostasis and accelerates diet-induced hyperlipidaemia in zebrafish larvae

Abstract

Hyperglycaemia damages the microvasculature in part through the reduced recruitment of immune cells and interference with platelet signalling, leading to poor wound healing and accelerated lipid deposition in mammals. We investigated the utility of zebrafish embryos to model the effect of glucose on leukocyte recruitment, thrombosis, and hyperlipidaemia by treating embryos with exogenous glucose. Exogenous glucose supplementation reduced neutrophil recruitment, and thrombocyte and fibrin plug formation following tail transection in zebrafish embryos, but did not affect the recruitment of macrophages. We observed accelerated lipid accumulation in glucose-supplemented embryos challenged with high fat chicken egg yolk feeding. Our study identifies conserved and divergent effects of high glucose in the tractable zebrafish embryo model system compared to mammals.

Transplantation of high fat fed mouse microbiota into zebrafish larvae identifies MyD88-dependent acceleration of hyperlipidaemia by Gram positive cell wall components

“…We next collected colonised zebrafish larvae and stained with Oil Red O to visualise neutral lipids but did not observe any differences in lipid staining. To determine if colonisation with the HFD-fed mouse microbiota affected the zebrafish response to challenge with a chicken egg yolk challenge [13], we challenged 5 dpf zebrafish with chicken egg yolk and stained with Oil Red O to visualise neutral lipids (Figure 1D). Zebrafish larvae colonised with the faecal microbiota from HFD fed mice had more vascular Oil Red O staining after 2 day of chicken egg yolk feeding challenge compared to zebrafish larvae colonised with the faecal microbiota from chow diet fed mice (Figure 1E).…”

“…High fat diet challenge with chicken egg yolk was performed as previously described [13]. Briefly, 5 dpf zebrafish embryos were placed in an E3 solution containing 0.05% w/v emulsified hard boiled chicken egg yolk in glass beakers.…”

“…Oil Red O staining and analysis was performed as previously described [13, 27, 28]. Briefly, 7 dpf embryos were fixed overnight at 4°C in 4% paraformaldehyde, rinsed with PBS, and rinsed stepwise through to propylene glycol.…”

Transplantation of high fat fed mouse microbiota into zebrafish larvae identifies MyD88‐dependent acceleration of hyperlipidaemia by Gram‐positive cell wall components

“…Oil Red O staining and analysis was performed as previously described 13,17,33 . Briefly, 7 dpf embryos were fixed overnight at 4°C in 4% paraformaldehyde, rinsed with PBS, and rinsed stepwise through to propylene glycol.…”

“…High fat diet challenge with chicken egg yolk was performed as previously described. 13 Briefly, 5 dpf zebrafish embryos were placed in an E3 solution containing 0.05% wt/vol emulsified hard boiled chicken egg yolk in glass beakers. Beakers were housed in a 28 C incubator with a 14:10 h light: dark cycle.…”