Glucose-limited high cell density cultivations from small to pilot plant scale using an enzyme-controlled glucose delivery system

Process optimization of high-level extracellular production of alkaline pectate lyase in recombinant Escherichia coli BL21 (DE3)

“…In this study, F t was adjusted every half an hour to simulate the exponential feeding method. In the pre-induction stage, there are no significant metabolic changes in response to the product formation or high cell density, and the cells can maintain a high level of glycerol consumption capacity [25]. According to this, we also assumed that the yield rate of glycerol to biomass (Y X/S ) was constant, whose value was the average yield rate over the whole process of exponential feeding and calculated by several experiment trials.…”

Liquid Perfluorochemicals as Flexible and Efficient Gas Carriers Applied in Bioprocess Engineering: An Updated Overview and Future Prospects

“…It results in a significant acidification of culture medium and in increase of its osmolality due to the dissolution of bicarbonate anions (Glazyrina et al, 2012;Zhu et al, 2005).…”

Increased Bacterial Cell Density and Recombinant Protein Yield Using a Commercial Microbial Cultivation System

“…[10] In addition, culture conditions allow the duration of induction to be increased providing greater protein processing time which may result in an increase in yield of soluble bioactive product. [10][11][12] EnBase media allow high cellular yields in small-scale cultures but have also been successfully used in wave bag cultures up to 1 L, [3] in small-scale stirred-cell reactors, [13] and as a starter culture for pilot-scale fed-batch bioreactor cultivation of alcohol dehydrogenase (ADH). [12] In the latter study the authors suggest that a continuum from micro-scale to pilot scale is possible due to comparable glucose delivery rates, achievable by varying hydrolytic enzyme (EnZ I'm) concentration.…”

“…[10][11][12] EnBase media allow high cellular yields in small-scale cultures but have also been successfully used in wave bag cultures up to 1 L, [3] in small-scale stirred-cell reactors, [13] and as a starter culture for pilot-scale fed-batch bioreactor cultivation of alcohol dehydrogenase (ADH). [12] In the latter study the authors suggest that a continuum from micro-scale to pilot scale is possible due to comparable glucose delivery rates, achievable by varying hydrolytic enzyme (EnZ I'm) concentration. [14] 218 G. R. Peck et al Capripoxviruses are Poxviridae that cause severe disease in sheep, goats, and cattle resulting in morbidity, mortality, and considerable economic loss (reviewed in reference [15]) They are enveloped viruses that contain a linear 150-kbp dsDNA genome that encodes approximately 156 proteins.…”

“…Previous studies of EnBase media performance have evaluated biomass yield, [11][12][13] protein yield, [2,13] protein solubility, [11,12] capacity for changes in production scale and culture vessel, [3,12,14] media composition and metabolites, [7,9,10] compatibility with high-throughput production, [18] and protein activity. [8] This study assessed the effect of EnBase media compared with Luria-Bertani (LB) media on attributes of bacterial cell yield and protein yield, solubility, and immunoreactivity of two recombinant capripoxvirus proteins.…”