Glucose Metabolism in Rat Brain In Vivo

Vrba, R.

doi:10.1038/195663a0

Cited by 51 publications

(9 citation statements)

References 9 publications

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“…Under normal conditions the glucose molecule contributes carbon atoms to the synthesis of these substrates (Geiger, Horvath, and Kawakita, 1960;Barkulis, Geiger, Kawakita, and Aguilar, 1960;Vrba, 1962). Oxidation continues in the absence of glucose but resynthesis of the endogenous substrates is not possible and there is thus loss of tissue constituents.…”

Section: Discussionmentioning

confidence: 99%

Effects of neonatal hypoglycaemia on the nervous system: a pathological study.

Anderson¹,

Milner²,

Strich³

1967

Journal of Neurology, Neurosurgery & Psychiatry

174

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Section: Discussionmentioning

confidence: 99%

Effects of neonatal hypoglycaemia on the nervous system: a pathological study.

Anderson¹,

Milner²,

Strich³

1967

Journal of Neurology, Neurosurgery & Psychiatry

174

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“…In the above studies the relatively neglected question is to what extent the labelled glutamate and aspartate formed from [U-'4C]glucose are also incorporated into the protein of the brain. Measureable and increasing amounts of ''C-labelled glutamate and aspartate were found in the brain protein only from 10 to 30 min after the subcutaneous injection of [U-14C]glucose (ROBERTS et a/., 1959;VRBA et a/., 1962;MINARD & RICHTER, 1968;VRBA & CANNON. 1970) despite the fact that very high values of specific radioactivities of the two above mentioned amino acids in the acid soluble extract of the brain are found within 5-10 min after the subcutaneous injection of [U-'4C]glucose (GAITONDE et a/., 1965).…”

mentioning

confidence: 99%

LABELLING OF BRAIN PROTEINS AT EARLY PERIODS AFTER SUBCUTANEOUS INJECTION OF A MIXTURE OF [U‐¹⁴C]GLUCOSE AND [³H]GLUTAMATE

Gaitonde

Harms

Evans

1978

Journal of Neurochemistry

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Abstract— To obtain evidence of the site of conversion of [U‐14C]glucose into glutamate and related amino acids of the brain, a mixture of [U‐14C]glucose and [3H]glutamate was injected subcutaneously into rats. [3H]Glutamate gave rise to several 3H‐labelled amino acids in rat liver and blood; only 3H‐labelled glutamate, glutamine or γ‐aminobutyrate were found in the brain. The specific radioactivity of [3H]glutamine in the brain was higher than that of [3H]glutamate indicating the entry of [3H]glutamate mainly in the ‘small glutamate compartment’. The 14C‐labelling pattern of amino acids in the brain and liver after injection of [U‐14C]glucose was similar to that previously reported (Gaitondeet al., 1965). The specific radioactivity of [14C]glutamine in the blood and liver after injection of both precursors was greater than that of glutamate between 10 and 60 min after the injection of the precursors. The extent of labelling of alanine and aspartate was greater than that of other amino acids in the blood after injection of [U‐14C]glucose. There was no labelling of brain protein with [3H]glutamate during the 10 min period, but significant label was found at 30 and 60 min. The highest relative incorporation of [14C]glutamate and [14C]aspartate in rat brain protein was observed at 5 min after the injection of [U‐14C]glucose. The results have been discussed in the context of transport of glutamine synthesized in the brain and the site of metabolism of [U‐14C]glucose in the brain.

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“…proteins of brain and numerous other (probably At present there are no comparative all) organs become rapidly labelled after sub-data available on rates of incorporation of cutaneous injection of [ u -'~c ]~~~~~~ in the [U-'4Clglucose carbon into proteins as o p rat and mouse (1)(2)(3)(4). Relevant literature has posed to lipids in brain.…”

Section: Introductionmentioning

confidence: 95%

“…in 0.7 ml saline per 100 g of body weight). Groups of four animals were killed by decapitation at intervals of 1,2,4,8,16,33,60, and 153 h after injection of [U-"C]glucose. The brains, livers, and hearts were rapidly excised, frozen in liquid N,, weighed to the nearest 0.01 g, and kept at -20" until homogenization.…”

Section: Labelling Of Rat Organs With [U-'"c]glucose In Vivomentioning

confidence: 99%

Movement of [U-¹⁴C]glucose Carbon into and Subsequent Release from lipids and High-Molecular-Weight Constituents of Rat Brain, Liver, and Heart In Vivo

Vrba¹,

Winter²

1972

Can. J. Biochem.

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VRBA, R., and WINTER, A. Movement of [CJ-'4C]glucsse carbon into and subsequent release from lipids and high-molecular-weight constituents of rat brain, liver, and heart in uiuo. Can. J. Biochem, 90, 91-105 (1972).After subcutaneous injection of [U-'4Cklucose into rats the amount of 14C incorporated in uiuo into proteins was always higher than into lipids in brain, liver, and heart. The specific radioactivity s f brain proteins was higher than those of liver and heart. Blood-brain comparisons show that protein carbon is derived continuously from glucose in the brain in sits and not as a result of deposition s f amino acids or proteins from the circulation. Seventy-two percent of I4C in purified brain protein fractions was found in the amino acids of the hydrolysates of these fractions, mainly in alanine, glutamk, and aspartic acids. Maximum labelling was reached about 4 h after injection of [U-'4C]glucose. Elimination of 14C from three classes of brain proteins (high-speed supernatant, particulate deoxycholate extractable, and residual) followed a biphasic timecourse. The extent of labelling of, and the rate of elimination of I4C from, the three classes of rat brain proteins were very similar. The fate of W in the other investigated tissue fractions of brain, liver, and heart was compared with the fate of "C in brain proteins.The results lend further support to the previously published suggestion that: (a) brain does not contain appreciable amounts of metabolically inert proteins or of proteins with turnover rates significantly higher than the mean for the bulk of brain proteins; (b) glucose carbon participates at a different rate and to a different extent in the metabolism of high-molecular-weight constituents of brain as compared to liver, heart, and plasma proteins; (c) the continuous conversion of glucose carbon into protein is an important part s f the maintenance of the homeostasis of tissue proteins in vivo. VRBA, R., et WINTER, A. Movement of [CJ-"C]glucose carbon into and subsequent release from lipids and Analysis of Hydrolysates of{U-'"Qlucose-hbelledPurijied Brain Proteins About 20 mg of "C-labelled rat brain proteins, prepared as described above and dissolved in 2 ml of 0.5 M NaOH, were hydrolyzed by addition of 2 ml of 12 M HQ and heating at 115" for 24 h in sealed test tubes. The hydrolysate Can. J. Biochem. Downloaded from www.nrcresearchpress.com by YALE MEDICAL LIBRARY on 12/29/14For personal use only.

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Glucose Metabolism in Rat Brain In Vivo

Cited by 51 publications

References 9 publications

Effects of neonatal hypoglycaemia on the nervous system: a pathological study.

Effects of neonatal hypoglycaemia on the nervous system: a pathological study.

LABELLING OF BRAIN PROTEINS AT EARLY PERIODS AFTER SUBCUTANEOUS INJECTION OF A MIXTURE OF [U‐¹⁴C]GLUCOSE AND [³H]GLUTAMATE

Movement of [U-¹⁴C]glucose Carbon into and Subsequent Release from lipids and High-Molecular-Weight Constituents of Rat Brain, Liver, and Heart In Vivo

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Glucose Metabolism in Rat Brain In Vivo

Cited by 51 publications

References 9 publications

Effects of neonatal hypoglycaemia on the nervous system: a pathological study.

Effects of neonatal hypoglycaemia on the nervous system: a pathological study.

LABELLING OF BRAIN PROTEINS AT EARLY PERIODS AFTER SUBCUTANEOUS INJECTION OF A MIXTURE OF [U‐14C]GLUCOSE AND [3H]GLUTAMATE

Movement of [U-14C]glucose Carbon into and Subsequent Release from lipids and High-Molecular-Weight Constituents of Rat Brain, Liver, and Heart In Vivo

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LABELLING OF BRAIN PROTEINS AT EARLY PERIODS AFTER SUBCUTANEOUS INJECTION OF A MIXTURE OF [U‐¹⁴C]GLUCOSE AND [³H]GLUTAMATE

Movement of [U-¹⁴C]glucose Carbon into and Subsequent Release from lipids and High-Molecular-Weight Constituents of Rat Brain, Liver, and Heart In Vivo