Glucose Metabolism of Clostridium Perfringens: Existence of a Metallo-Aldolase

Bard, R. C.; Gunsalus, I. C.

doi:10.1128/jb.59.3.387-400.1950

Cited by 131 publications

(54 citation statements)

References 20 publications

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“…Included would be impairment of those enzymes which contain SH groups essential for their activity. Both GAP-DH and aldolase are such enzymes (1,7,12,22).…”

Section: Resultsmentioning

confidence: 99%

Effect of sodium nitrite inhibition on intracellular thiol groups and on the activity of certain glycolytic enzymes in Clostridium perfringens

O'leary¹,

Solberg²

1976

Appl Environ Microbiol

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Activities of glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) (GAP-DH) and aldolase (EC 4.1.2.13) in cells of Clostridium perfringens that had been inhibited with sodium nitrite were investigated. A complete loss in GAP-DH activity and a 67% decrease in aldolase activity were observed when growth of C. perfringens was inhibited. There was also a 91% decrease in the concentration of free sulfhydryl groups of soluble cellular components. Dithiothreitol restored some activity to inactive GAP-DH from sodium nitrite-inhibited cells, indicating that a loss of reduced sulfhydryl groups was involved in the inactivation of the enzyme. The evidence presented suggests that sodium nitrite inhibition of C. perfringens may involve an interaction of sodium nitrite as nitrous acid with sulfhydryl-containing constituents of the bacterial cell.

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“…Included would be impairment of those enzymes which contain SH groups essential for their activity. Both GAP-DH and aldolase are such enzymes (1,7,12,22).…”

Section: Resultsmentioning

confidence: 99%

Effect of sodium nitrite inhibition on intracellular thiol groups and on the activity of certain glycolytic enzymes in Clostridium perfringens

O'leary¹,

Solberg²

1976

Appl Environ Microbiol

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“…Of these, H. halobium, H. salinarium, H. cutirubrum, H. mediterranei and H. volcanii possess, like most microbes [1,4], an EDTA-sensitive, class II aldolase activity. The bivalent metal preferred by the class II halobacterial aldolases is Fe 2÷, as in the case of Clostridium perfringens and cyanobacteria [21,22].…”

Section: Discussionmentioning

confidence: 99%

Distribution of class I and class II fructose biphosphate aldolases in halophilic archaebacteria

Dhar

Altekar

1986

FEMS Microbiology Letters

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Both class I (Schiff base-forming) and class II (metal-requiring) fructose biphosphate (FDP) aldolases were found to be distributed among halophilic archaebacteria. The type of enzyme did not vary with the growth medium. The aldolase activities were also halophilic.the c-amino group of a lysine residue of the enzyme [4]. They are usually found to be present in higher eukaryotes though certain exceptions of 'unusual' class I bacterial aldolases are known [5][6][7][8][9]. A class I aldolase from an archaebacterial [10] source is now shown [3]. Therefore, we have examined the aldolase in various halobacteria and the distribution of class I and II aldolases among halobacteria is described.

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“…Another and more intriguing possibility, however, is that lactate may participate directly in a Stickland reaction with proline, the requirements for such activity being amino acid-fermenting organisms possessing lactate dehydrogenase. Although organisms of the genus Clostridium (2) and at least one member of the genus Peptostreptococcus (17) are known to contain lactate dehydrogenase activity, Stickland reactions involving lactate as the hydrogen donor have thus far not been reported. We are currently examining oral isolates for their ability to utilize lactate in Stickland reactions.…”

mentioning

confidence: 99%

Stickland reactions of dental plaque

Curtis¹,

Kemp²,

Robrish³

et al. 1983

Infect Immun

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Dental plaque samples from monkeys (Macaca fascicularis) were shown to contain proline reduction activity in coupled Stickland reactions with other amino acids and also with certain end products of bacterial glucose metabolism. The unusually high concentration of bound and free proline in the oral environment may be of importance in both the production of base and in the removal of acid from the tooth surface after dietary carbohydrate ingestion.

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Glucose Metabolism of Clostridium Perfringens: Existence of a Metallo-Aldolase

Cited by 131 publications

References 20 publications

Effect of sodium nitrite inhibition on intracellular thiol groups and on the activity of certain glycolytic enzymes in Clostridium perfringens

Effect of sodium nitrite inhibition on intracellular thiol groups and on the activity of certain glycolytic enzymes in Clostridium perfringens

Distribution of class I and class II fructose biphosphate aldolases in halophilic archaebacteria

Stickland reactions of dental plaque

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