Glucose oxidase overproducing mutants ofPenicillium variabile(P16)

Abstract

Conidia of Penicillium variabile P16 were subjected to mutagenesis and selection for glucose oxidase production on media containing o‐dianisidine. Studies of the relationship between dose of UV irradiation and conidial survival and frequency of mutation showed that the best frequency of positive mutation (17%) was obtained in correspondence to a conidial survival of 52%. Out of 54 overproducing mutants tested in shaken flasks, M‐80.10 showed the highest level of glucose oxidase activity (127% higher than the wild‐type). M‐80.10 mutant, transferred every 15 days to fresh medium and tested monthly for 8 months, appeared stable. The time course of growth and enzyme production by the mutant M‐80.10 showed an increase of the glucose oxidase activity in the culture medium up to 19 U ml−1 after 96 h of fermentation.

Effect of dimorphic regulation on heterologous glucose oxidase production by Mucor circinelloides

“…The heterologous protein production was therefore not growth-related, since GOX production mostly occurred during the stationary phase of mycelial growth. Production of GOX during the stationary phase of mycelial growth was contrary to the growth-related GOX production observed in native fungal systems (Kona et al, 2001;Petruccioli et al, 1995), and the glucose-induced characteristics of the gpd1 promoter (Larsson et al, 2004). The secretion of GOX by recombinant Mucor was particularly poor.…”

Selective Propargylation of Carbonyl Compounds and Imines with Barium Reagents

“…The heterologous protein production was therefore not growth‐related, since GOX production mostly occurred during the stationary phase of mycelial growth. Production of GOX during the stationary phase of mycelial growth was contrary to the growth‐related GOX production observed in native fungal systems (Kona et al , 2001; Petruccioli et al , 1995), and the glucose‐induced characteristics of the gpd1 promoter (Larsson et al , 2004). The secretion of GOX by recombinant Mucor was particularly poor.…”

Selective isolation of Aspergillus niger mutants with enhanced glucose oxidase production

“…1995). In this respect, our result can be compared with data compiled by Petruccioli et al (1995), who improved GOD production over 127% after mutagenesis of P. oarzahile. Similar results (over twofold increase of GOD activity) were reached by Markwell et ul.…”

“…Literature reports the possibility of obtaining high effectiveness of G O D synthesis in nzger and Penirillium notutum mutants, for instance, by induction with various mutagens (Markwell et al 1989;Fiedurek et al 1990;Petruccioli et a/. 1995).…”