Glucose oxidase release from calcium alginate gel capsules

Blandino, Ana; Macı́as, M.; Cantero, Domingo

doi:10.1016/s0141-0229(00)00204-0

Cited by 158 publications

(119 citation statements)

References 22 publications

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“…The results were in agreement with previous studies [12,[15][16][17]. It has been reported that the membrane thickness of the capsules was increased as the calcium chloride concentration increased [12,16,17].…”

Section: Influence Of Liquid Core Solution Formulationsupporting

confidence: 93%

“…The membrane thickness of the capsules also exhibited a decrease as alginate concentration increased (see Figure 3B) for a given gelation time. The results were in good agreement with previous studies [12,16,17]. At high alginate concentrations, number of alginate molecules per unit volume and the number of binding sites for Ca 2+ cation also increased.…”

Section: Influence Of Alginate Solution Formulationsupporting

confidence: 92%

“…The reason could be that the calcium chloride concentration tested in this study was relatively low ( < 15 g/l) compared to previous studies [12,16]. The previous studies reported the capsule diameter produced from calcium chloride concentration in the range of 13 g/l to 130 g/l [12,16]. Because low concentration of CaCl 2 was used in this study, it is possible that the calcium cations are not in excess to react with alginate molecules at a given fixed gelation time.…”

Section: Discussionmentioning

confidence: 70%

“…As shown in Figure 2A, the diameter of the capsule was neither influenced by calcium chloride concentration nor by CMC concentration. The reason could be that the calcium chloride concentration tested in this study was relatively low ( < 15 g/l) compared to previous studies [12,16]. The previous studies reported the capsule diameter produced from calcium chloride concentration in the range of 13 g/l to 130 g/l [12,16].…”

Section: Discussionmentioning

confidence: 73%

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Alginate liquid core capsule formation using the simple extrusion dripping method

Lee

Ibrahim

Chu

et al. 2014

Journal of Polymer Engineering

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Liquid core capsules have been widely used in various biotechnological applications. The capsules could be formed by the simple extrusion dripping method. However, the method requires strict control of several process variables in order to form spherical capsules. The aim of this study was to systematically investigate the influence of process variables of the method on the capsule size and shape. The results showed that the capsules diameter was decreased when the concentration of alginate solution was increased. The capsule diameter was increased when the gelation time and dripping tip diameter were increased. The membrane thickness of the capsules was significantly increased by the concentration of calcium chloride, gelation time and dripping tip diameter. However, the concentration of alginate gave the opposite trend on the membrane thickness of the capsules. As a recommendation, uniform and spherical alginate liquid core capsules could be formed when concentration of calcium chloride was > 10 g/l, the concentration of alginate solution was > 5 g/l and < 20 g/l, gelation solution height in between 1.7 cm and 3.2 cm, and stirring rate of the gelation bath was in the range of 400-500 rpm.

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Section: Influence Of Liquid Core Solution Formulationsupporting

confidence: 93%

Section: Influence Of Alginate Solution Formulationsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 73%

See 2 more Smart Citations

Alginate liquid core capsule formation using the simple extrusion dripping method

Lee

Ibrahim

Chu

et al. 2014

Journal of Polymer Engineering

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“…On the other hand, the procedure had its own limitations such as the low stability and high porosity of the membrane. 78,79 It was observed that the calcium alginate gels did not provide any serious barrier to the diffusion of neutral substances up to five hundred molecular weight. 78 The diffusion of glucose oxidase, an enzyme with high molecular weight, out of calcium alginate gel capsules was also studied.…”

Section: ·7 Enzyme Immobilizationmentioning

confidence: 99%

Recent Developments, Characteristics, and Potential Applications of Electrochemical Biosensors

2004

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The objective of this study is to analyze the technical importance, performance, techniques, advantages, and disadvantages of the biosensors in general and of the electrochemical biosensors in particular. A product of reaction diffuses to the transducer in the first generation biosensors (based on Clark biosensors). The mediated biosensors or second generation biosensors use specific mediators between the reaction and the transducer to improve sensitivity. The second generation biosensors involve two steps: first, there is a redox reaction between enzyme and substrate that is reoxidized by the mediator, and eventually the mediator is oxidized by the electrode. No normal product or mediator diffusion is directly involved in the third generation biosensors, direct biosensors. Based on the type of transducer, current biosensors are divided into optical, mass, thermal, and electrochemical sensors. They are used in medical diagnostics, food quality controls, environmental monitoring, and other applications. These biosensors are also grouped under two broad categories of sensors: direct and indirect detection systems. Moreover, these systems could be further grouped into continuous or batch operation. Therefore, amperometric biosensors and their current applications are focused on more in detail since they are the most commonly used biosensors in monitoring and diagnosing tests in clinical analysis. Problems related to the commercialization of medical, environmental, and industrial biosensors as well as their performance characteristics, their competitiveness in comparison to the conventional analytical tools, and their costs determine the future development of these biosensors.

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Rapid coating of millimeter‐scale hydrogel capsules with interfacial polymerization

Pan,

Huang,

Gong

et al. 2024

J of Applied Polymer Sci

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A rapid and mild coating strategy was applied on the millimeter scale calcium alginate capsules (Ca‐SA caps) by in situ interfacial polymerization of ethyl cyanoacrylate (ECA). The generated poly(ethyl cyanoacrylate) (PECA) coating was initiated by trace water in the surface of the Ca‐SA caps. The effects of ECA content, reaction time, and type of aromatic solvent on the morphology and control‐release property of the resulting PECA‐coated Ca‐SA caps (Ca‐SA caps@PECA) were investigated. The results demonstrated a white PECA layer successfully coated on the surface of Ca‐SA caps. Notably, the Ca‐SA caps@PECA remained consistently pleasing spherical shape as prepared in methylbenzene and ethylbenzene. When the ECA was 6 mL, the thickness of PECA layer of the Ca‐SA caps@PECA was 43.52 μm. Compared with the Ca‐SA caps, the weight retention ratio of water in the Ca‐SA caps@PECA increased by 254.61%, indicating the effective delay of water diffusion from core of the Ca‐SA caps@PECA. Moreover, the PECA coating improved the stability of the Ca‐SA caps@PECA, which achieved release‐controlling of dye at different pH. This method provides solution for surface modification of fragile hydrogel capsules to effectively control the releasing of liquid core, which is highly valuable in the field of food, cosmetic, and medical delivery.

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Glucose oxidase release from calcium alginate gel capsules

Cited by 158 publications

References 22 publications

Alginate liquid core capsule formation using the simple extrusion dripping method

Alginate liquid core capsule formation using the simple extrusion dripping method

Recent Developments, Characteristics, and Potential Applications of Electrochemical Biosensors

Rapid coating of millimeter‐scale hydrogel capsules with interfacial polymerization

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