Glucose oxidation by osmium redox polymer mediated enzyme electrodes operating at low potential and in oxygen, for application to enzymatic fuel cells

Osadebe, Isioma; Conghaile, Peter Ó; Kavanagh, Paul; Leech, Dónal

doi:10.1016/j.electacta.2015.09.088

Cited by 24 publications

(20 citation statements)

References 45 publications

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“…Osmium‐based mediators have been widely used owing to the ability to modulate the mediator redox potential of the central Os metal by using coordinating ligands, the relative stability of the resulting complexes in the Os(II)/Os(III) states, and because the hydrogel characteristics of redox‐polymer films permit rapid mass and charge transport, thus generating substantial current signals . The inclusion of multi‐walled carbon nanotubes (MWCNTs) is one route towards improving glucose oxidation currents for enzyme electrodes prepared by crosslinking the nanomaterial with enzymes and osmium‐based redox mediators , attributed to improved retention of enzyme activity . Tsujimura et al .…”

Section: Introductionmentioning

confidence: 99%

Design of Experiments Approach to Provide Enhanced Glucose‐oxidising Enzyme Electrode for Membrane‐less Enzymatic Fuel Cells Operating in Human Physiological Fluids

et al. 2018

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Graphite electrodes are modified with a redox polymer, [Os(4,4′‐dimethoxy‐2,2′‐bipyridine)2(polyvinylimidazole)10Cl]+ (E°′=−0.02 V vs Ag/AgCl (3 M KCl), crosslinked with a flavin adenine dinucleotide glucose dehydrogenase and multi‐walled carbon nanotubes for electrocatalytic oxidation of glucose. The enzyme electrodes provide 52 % higher current density, 1.22±0.10 mA cm−2 in 50 mM phosphate‐buffered saline at 37 °C containing 5 mM glucose, when component amounts are optimised using a design of experiments approach compared to one‐factor‐at‐a‐time. Current densities of 0.84±0.15 mA cm−2 were achieved in the presence of oxygen for these enzyme electrodes. Further analysis of the model allowed for altering of the electrode components while maintaining similar current densities, 0.78±0.11 mA cm−2 with 34 % less enzyme. Application of the cost‐effective anodes in membrane‐less enzymatic fuel cells is demonstrated by connection to cathodes prepared by co‐immobilisation of [Os(2,2′‐bipyridine)2(polyvinylimidazole)10Cl+] redox polymer, Myrothecium verrucaria bilirubin oxidase and multi‐walled carbon nanotubes on graphite electrodes. Power densities of up to 285 μW cm−2, 146 μW cm−2 and 60 μW cm−2 are achieved in pseudo‐physiological buffer, artificial plasma and human plasma respectively, showing promise for in vivo or ex vivo power generation under these conditions.

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Section: Introductionmentioning

confidence: 99%

Design of Experiments Approach to Provide Enhanced Glucose‐oxidising Enzyme Electrode for Membrane‐less Enzymatic Fuel Cells Operating in Human Physiological Fluids

et al. 2018

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“…Several nanostructures have been also employed to favour the electron transfer rate. These include carbon materials, such as multi-walled carbon nanotubes (MWCNTs) [ 13 ], platinum or gold nanoparticles [ 10 ], and redox polymers [ 14 ]. Oxygen can also act as a mediator, however, challenges such as low concentrations of oxygen and the crossover between anode and cathode, might arise [ 15 ].…”

Section: Enzymatic Fuel Cells For Biosensing Applicationsmentioning

confidence: 99%

“…Enzymes with deeply buried redox centres, such as glucose oxidase (GOx), must be aided by mediators to connect the enzyme’s redox centre to the electrode and/or overcome their distance [ 16 ]. The several strategies implemented include the use of molecular wires and artificial cofactor derivatives [ 9 , 14 ]. A limitation of GOx is that it can use oxygen as the electron acceptor.…”

Section: Enzymatic Fuel Cells For Biosensing Applicationsmentioning

confidence: 99%

“…Osmium polymers have also been widely explored in EFCs, due to their fast electron transfer rates and tunable redox potential [ 14 , 43 ]. Cadet et al developed a glucose/oxygen fuel cell based on an osmium polymer, functionalised with glucose dehydrogenase, in the case of the anode, and bilirubin oxidase, in the case of the cathode [ 44 ].…”

Section: Implantable Enzymatic Fuel Cellsmentioning

confidence: 99%

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Enzymatic Fuel Cells: Towards Self-Powered Implantable and Wearable Diagnostics

2018

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With the rapid progress in nanotechnology and microengineering, point-of-care and personalised healthcare, based on wearable and implantable diagnostics, is becoming a reality. Enzymatic fuel cells (EFCs) hold great potential as a sustainable means to power such devices by using physiological fluids as the fuel. This review summarises the fundamental operation of EFCs and discusses the most recent advances for their use as implantable and wearable self-powered sensors.

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“…Since the initial report on the use of ferrocene and its derivatives (Cass et al, 1984), osmium-based redox polymers have been widely utilized in enzymatic devices (Aquino Neto and De Andrade, 2013). Osmium redox polymer mediators have the flexibility that their redox potential can be tailored conveniently by altering the ligands attached to the osmium central metal atom (Forster and Vos, 1990, Gallaway and Calabrese Barton, 2008and Sakai et al, 2009 giving a broad voltage range that makes them suitable for both anode and cathode processes in glucose/ O 2 enzymatic fuel cells (Rengaraj et al, 2011;Osadebe et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Paper-based microfluidic biofuel cell operating under glucose concentrations within physiological range

González-Guerrero

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et al. 2017

Biosensors and Bioelectronics

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This work addresses the development of a compact paper-based enzymatic microfluidic glucose/O fuel cell that can operate using a very limited sample volume (≈35µl) and explores the energy generated by glucose at concentrations typically found in blood samples at physiological conditions (pH 7.4). Carbon paper electrodes combined with a paper sample absorption substrate all contained within a plastic microfluidic casing are used to construct the paper-based fuel cell. The anode catalysts consist of glucose dehydrogenase and [Os(4,4'-dimethoxy-2,2'-bipyridine)(poly-vinylimidazole)Cl] as mediator, while the cathode catalysts were bilirubin oxidase and [Os(2,2'-bipyridine)(poly-vinylimidazole)Cl] as mediator. The fuel cell delivered a linear power output response to glucose over the range of 2.5-30mM, with power densities ranging from 20 to 90µWcm. The quantification of the available electrical power as well as the energy density extracted from small synthetic samples allows planning potential uses of this energy to power different sensors and analysis devices in a wide variety of in-vitro applications.

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Glucose oxidation by osmium redox polymer mediated enzyme electrodes operating at low potential and in oxygen, for application to enzymatic fuel cells

Cited by 24 publications

References 45 publications

Design of Experiments Approach to Provide Enhanced Glucose‐oxidising Enzyme Electrode for Membrane‐less Enzymatic Fuel Cells Operating in Human Physiological Fluids

Design of Experiments Approach to Provide Enhanced Glucose‐oxidising Enzyme Electrode for Membrane‐less Enzymatic Fuel Cells Operating in Human Physiological Fluids

Enzymatic Fuel Cells: Towards Self-Powered Implantable and Wearable Diagnostics

Paper-based microfluidic biofuel cell operating under glucose concentrations within physiological range

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